Gene description for VAMP5
Gene name vesicle-associated membrane protein 5
Gene symbol VAMP5
Other names/aliases -
Species Homo sapiens
 Database cross references - VAMP5
ExoCarta ExoCarta_10791
Vesiclepedia VP_10791
Entrez Gene 10791
HGNC 12646
MIM 607029
UniProt O95183  
 VAMP5 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for VAMP5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    muscle organ development GO:0007517 TAS
    skeletal muscle tissue development GO:0007519 IEA
    cell differentiation GO:0030154 IEA
    Golgi to plasma membrane protein transport GO:0043001 IBA
Subcellular Localization
    late endosome GO:0005770 ISS
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 IDA
    intercalated disc GO:0014704 IEA
    cytoplasmic vesicle membrane GO:0030659 ISS
    organelle membrane GO:0031090 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified VAMP5 in exosomes
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for VAMP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCFD1 23256
Affinity Capture-MS Homo sapiens
2 VTI1B 10490
Affinity Capture-MS Homo sapiens
3 SNAP23 8773
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 NAPA 8775
Affinity Capture-MS Homo sapiens
5 TNPO1 3842
Affinity Capture-MS Homo sapiens
6 CD53 963
Two-hybrid Homo sapiens
7 MYO1F 4542
Affinity Capture-MS Homo sapiens
8 ASGR2  
Two-hybrid Homo sapiens
9 NBAS 51594
Affinity Capture-MS Homo sapiens
10 GOSR2 9570
Affinity Capture-MS Homo sapiens
11 STX4 6810
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 PTRH2 51651
Affinity Capture-MS Homo sapiens
13 IL6ST 3572
Affinity Capture-MS Homo sapiens
14 SLC38A5 92745
Affinity Capture-MS Homo sapiens
15 ITFG1 81533
Affinity Capture-MS Homo sapiens
16 TMPRSS2 7113
Two-hybrid Homo sapiens
17 STX1A 6804
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 RINT1 60561
Affinity Capture-MS Homo sapiens
19 SLC39A11  
Affinity Capture-MS Homo sapiens
20 STX8 9482
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
21 MUC1 4582
Two-hybrid Homo sapiens
22 STX7 8417
Affinity Capture-MS Homo sapiens
23 MDC1  
Two-hybrid Homo sapiens
24 KLRC1  
Two-hybrid Homo sapiens
25 ORC4  
Affinity Capture-MS Homo sapiens
26 STX18 53407
Affinity Capture-MS Homo sapiens
27 SCARB1 949
Affinity Capture-MS Homo sapiens
28 STX6 10228
Affinity Capture-MS Homo sapiens
29 TMEM192 201931
Affinity Capture-MS Homo sapiens
30 ITFG3 83986
Affinity Capture-MS Homo sapiens
31 TNFSF9  
Affinity Capture-MS Homo sapiens
32 LRFN1  
Affinity Capture-MS Homo sapiens
33 BET1 10282
Affinity Capture-MS Homo sapiens
34 KIR2DL3  
Two-hybrid Homo sapiens
35 GPX8 493869
Two-hybrid Homo sapiens
36 STX12 23673
Affinity Capture-MS Homo sapiens
37 PDS5B 23047
Affinity Capture-MS Homo sapiens
38 VTI1A 143187
Affinity Capture-MS Homo sapiens
39 MICA 100507436
Affinity Capture-MS Homo sapiens
40 TMEM106C 79022
Two-hybrid Homo sapiens
41 KIR3DL3  
Two-hybrid Homo sapiens
42 CLDN7 1366
Two-hybrid Homo sapiens
43 TMEM14B  
Two-hybrid Homo sapiens
44 TMCO5A  
Two-hybrid Homo sapiens
45 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
46 BNIP1 662
Affinity Capture-MS Homo sapiens
47 VPS45 11311
Affinity Capture-MS Homo sapiens
48 JAGN1 84522
Two-hybrid Homo sapiens
49 ERGIC2 51290
Affinity Capture-MS Homo sapiens
50 BET1L 51272
Affinity Capture-MS Homo sapiens
51 MCAM 4162
Proximity Label-MS Homo sapiens
52 VSIG4  
Two-hybrid Homo sapiens
53 SNAP47 116841
Affinity Capture-MS Homo sapiens
54 GORAB 92344
Two-hybrid Homo sapiens
55 TNPO2 30000
Affinity Capture-MS Homo sapiens
56 PDS5A 23244
Affinity Capture-MS Homo sapiens
57 HMOX2 3163
Affinity Capture-MS Homo sapiens
58 TMEM45B 120224
Two-hybrid Homo sapiens
59 NAPG 8774
Affinity Capture-MS Homo sapiens
60 ATP4A 495
Affinity Capture-MS Homo sapiens
61 STX5 6811
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
62 SNAP29 9342
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
63 ULBP3 79465
Affinity Capture-MS Homo sapiens
64 TMEM52B 120939
Two-hybrid Homo sapiens
65 SEC63 11231
Affinity Capture-MS Homo sapiens
66 GJA8  
Two-hybrid Homo sapiens
67 TRHDE 29953
Affinity Capture-MS Homo sapiens
68 FNDC9  
Two-hybrid Homo sapiens
69 TAS2R5  
Two-hybrid Homo sapiens
70 VAMP8 8673
Affinity Capture-MS Homo sapiens
71 PANX1 24145
Two-hybrid Homo sapiens
72 TM4SF18  
Two-hybrid Homo sapiens
73 LRRC25  
Two-hybrid Homo sapiens
74 SLC14A2  
Two-hybrid Homo sapiens
75 ERGIC3 51614
Affinity Capture-MS Homo sapiens
76 LRFN3  
Affinity Capture-MS Homo sapiens
77 ATP6AP2 10159
Two-hybrid Homo sapiens
78 TMEM139  
Two-hybrid Homo sapiens
79 VAMP3 9341
Affinity Capture-MS Homo sapiens
80 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
81 GPR152  
Two-hybrid Homo sapiens
82 TMEM86B  
Two-hybrid Homo sapiens
83 CLDN5 7122
Two-hybrid Homo sapiens
84 ZW10 9183
Affinity Capture-MS Homo sapiens
85 MICB  
Affinity Capture-MS Homo sapiens
86 FAM134C 162427
Two-hybrid Homo sapiens
87 HIBADH 11112
Two-hybrid Homo sapiens
88 CPLX4  
Two-hybrid Homo sapiens
89 ABCA2 20
Affinity Capture-MS Homo sapiens
90 STX10 8677
Affinity Capture-MS Homo sapiens
91 STX17 55014
Affinity Capture-MS Homo sapiens
92 TMX2 51075
Two-hybrid Homo sapiens
93 STX2 2054
Two-hybrid Homo sapiens
94 FLVCR1 28982
Affinity Capture-MS Homo sapiens
95 ABHD16A 7920
Two-hybrid Homo sapiens
96 USE1 55850
Affinity Capture-MS Homo sapiens
97 STX16 8675
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
98 SCFD2 152579
Affinity Capture-MS Homo sapiens
99 GOSR1 9527
Affinity Capture-MS Homo sapiens
100 SPINT1 6692
Two-hybrid Homo sapiens
101 NME2P1 283458
Affinity Capture-MS Homo sapiens
102 FAM209A 200232
Two-hybrid Homo sapiens
103 C19orf25  
Affinity Capture-MS Homo sapiens
104 IL3RA  
Two-hybrid Homo sapiens
105 NAPB 63908
Affinity Capture-MS Homo sapiens
106 VAMP7 6845
Affinity Capture-MS Homo sapiens
107 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
108 GRAMD3  
Two-hybrid Homo sapiens
109 STX3 6809
Affinity Capture-MS Homo sapiens
110 SLC38A1 81539
Affinity Capture-MS Homo sapiens
111 YKT6 10652
Affinity Capture-MS Homo sapiens
112 TMEM31  
Two-hybrid Homo sapiens
113 SCAMP3 10067
Affinity Capture-MS Homo sapiens
114 ELOVL4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which VAMP5 is involved
No pathways found





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