Gene description for NAPB
Gene name N-ethylmaleimide-sensitive factor attachment protein, beta
Gene symbol NAPB
Other names/aliases SNAP-BETA
SNAPB
Species Homo sapiens
 Database cross references - NAPB
ExoCarta ExoCarta_63908
Vesiclepedia VP_63908
Entrez Gene 63908
HGNC 15751
MIM 611270
UniProt Q9H115  
 NAPB identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NAPB
Molecular Function
    soluble NSF attachment protein activity GO:0005483 IBA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IBA
Biological Process
    intracellular protein transport GO:0006886 IBA
    regulation of synaptic vesicle priming GO:0010807 IBA
    synaptic transmission, glutamatergic GO:0035249 IBA
    SNARE complex disassembly GO:0035494 IBA
Subcellular Localization
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 IBA
    extracellular exosome GO:0070062 HDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified NAPB in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NAPB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFEC  
Two-hybrid Homo sapiens
2 BET1 10282
Two-hybrid Homo sapiens
3 STX5 6811
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SDCBP 6386
Two-hybrid Homo sapiens
6 STX16 8675
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SNAP23 8773
Two-hybrid Homo sapiens
9 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GPM6A 2823
Affinity Capture-MS Homo sapiens
11 LEUTX  
Two-hybrid Homo sapiens
12 TAF1B  
Two-hybrid Homo sapiens
13 CISD2 493856
Two-hybrid Homo sapiens
14 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SIGLEC5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 TMEM139  
Two-hybrid Homo sapiens
17 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
18 HSD17B13  
Two-hybrid Homo sapiens
19 KCNN3  
Two-hybrid Homo sapiens
20 ARL6IP1 23204
Two-hybrid Homo sapiens
21 STX4 6810
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 STX19  
Two-hybrid Homo sapiens
23 CREB3L1  
Two-hybrid Homo sapiens
24 CLEC10A  
Two-hybrid Homo sapiens
25 APEX1 328
Affinity Capture-RNA Homo sapiens
26 FAM209A 200232
Two-hybrid Homo sapiens
27 STX1A 6804
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 STX3 6809
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ALOX5AP  
Two-hybrid Homo sapiens
32 VAMP5 10791
Affinity Capture-MS Homo sapiens
33 NAPG 8774
Affinity Capture-MS Homo sapiens
34 STX7 8417
Affinity Capture-MS Homo sapiens
35 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DGAT2L6  
Two-hybrid Homo sapiens
37 SYT2  
Two-hybrid Homo sapiens
38 GJB5  
Two-hybrid Homo sapiens
39 STX6 10228
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
40 STX2 2054
Two-hybrid Homo sapiens
View the network image/svg+xml



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