Gene description for NAPB
Gene name N-ethylmaleimide-sensitive factor attachment protein, beta
Gene symbol NAPB
Other names/aliases SNAP-BETA
SNAPB
Species Homo sapiens
 Database cross references - NAPB
ExoCarta ExoCarta_63908
Vesiclepedia VP_63908
Entrez Gene 63908
HGNC 15751
MIM 611270
UniProt Q9H115  
 NAPB identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NAPB
Molecular Function
    soluble NSF attachment protein activity GO:0005483 IBA
    syntaxin binding GO:0019905 IBA
    protein binding GO:0005515 IPI
Biological Process
    membrane fusion GO:0061025 IBA
    intracellular protein transport GO:0006886 IBA
    SNARE complex disassembly GO:0035494 IBA
Subcellular Localization
    vacuolar membrane GO:0005774 IBA
    SNARE complex GO:0031201 IBA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified NAPB in exosomes
1
Experiment ID 834
MISEV standards
EM
EV Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
EV Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NAPB
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NAPB is involved
No pathways found





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