Gene description for USE1
Gene name unconventional SNARE in the ER 1 homolog (S. cerevisiae)
Gene symbol USE1
Other names/aliases D12
MDS032
P31
SLT1
Species Homo sapiens
 Database cross references - USE1
ExoCarta ExoCarta_55850
Vesiclepedia VP_55850
Entrez Gene 55850
HGNC 30882
MIM 610675
UniProt Q9NZ43  
 USE1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for USE1
Molecular Function
    SNAP receptor activity GO:0005484 IBA
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISS
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    lysosomal transport GO:0007041 ISS
    protein transport GO:0015031 IEA
    protein catabolic process GO:0030163 ISS
    secretion by cell GO:0032940 ISS
    membrane fusion GO:0061025 IEA
Subcellular Localization
    lysosome GO:0005764 ISS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    COPI-coated vesicle GO:0030137 IEA
    SNARE complex GO:0031201 IBA
 Experiment description of studies that identified USE1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for USE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Proximity Label-MS Homo sapiens
3 BET1 10282
Affinity Capture-MS Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 BSCL2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 TTYH1  
Affinity Capture-MS Homo sapiens
8 POLDIP3 84271
Affinity Capture-MS Homo sapiens
9 NBAS 51594
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SNAP47 116841
Affinity Capture-MS Homo sapiens
11 PCDHB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 VTI1B 10490
Affinity Capture-MS Homo sapiens
13 RPN2 6185
Proximity Label-MS Homo sapiens
14 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SCFD2 152579
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 METTL7A 25840
Proximity Label-MS Homo sapiens
17 STX17 55014
Affinity Capture-MS Homo sapiens
18 MARCKS 4082
Proximity Label-MS Homo sapiens
19 STX3 6809
Affinity Capture-MS Homo sapiens
20 LRRC59 55379
Proximity Label-MS Homo sapiens
21 PANX1 24145
Proximity Label-MS Homo sapiens
22 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GOSR1 9527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NUP155 9631
Proximity Label-MS Homo sapiens
25 KIAA1715 80856
Proximity Label-MS Homo sapiens
26 PXMP2  
Proximity Label-MS Homo sapiens
27 NAT10 55226
Affinity Capture-MS Homo sapiens
28 Zw10  
Affinity Capture-MS Mus musculus
29 MEX3A  
Affinity Capture-RNA Homo sapiens
30 VAMP3 9341
Affinity Capture-MS Homo sapiens
31 SCFD1 23256
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 HSD3B7 80270
Proximity Label-MS Homo sapiens
34 YKT6 10652
Affinity Capture-MS Homo sapiens
35 STIM1 6786
Proximity Label-MS Homo sapiens
36 HSD17B11 51170
Proximity Label-MS Homo sapiens
37 EMD 2010
Proximity Label-MS Homo sapiens
38 DNAJC25 548645
Proximity Label-MS Homo sapiens
39 CSN2 1447
Affinity Capture-MS Homo sapiens
40 BCAP31 10134
Proximity Label-MS Homo sapiens
41 FKBP8 23770
Proximity Label-MS Homo sapiens
42 C19orf25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 NUP35 129401
Proximity Label-MS Homo sapiens
44 STX4 6810
Proximity Label-MS Homo sapiens
45 PDCD11 22984
Affinity Capture-MS Homo sapiens
46 GOSR2 9570
Affinity Capture-MS Homo sapiens
47 APP 351
Reconstituted Complex Homo sapiens
48 MGLL 11343
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RAB4A 5867
Proximity Label-MS Homo sapiens
50 APEX1 328
Affinity Capture-RNA Homo sapiens
51 BNIP1 662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ZW10 9183
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 B3GAT1  
Proximity Label-MS Homo sapiens
54 C3orf52  
Affinity Capture-MS Homo sapiens
55 HS2ST1 9653
Affinity Capture-MS Homo sapiens
56 SEC62 7095
Proximity Label-MS Homo sapiens
57 ATP2A1 487
Proximity Label-MS Homo sapiens
58 RINT1 60561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RAB7A 7879
Proximity Label-MS Homo sapiens
60 TRIM27  
Two-hybrid Homo sapiens
61 KXD1 79036
Affinity Capture-MS Homo sapiens
62 KCNN4 3783
Affinity Capture-MS Homo sapiens
63 RPN1 6184
Proximity Label-MS Homo sapiens
64 MEOX2  
Two-hybrid Homo sapiens
65 CREB3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 STX8 9482
Affinity Capture-MS Homo sapiens
67 Grsf1  
Protein-RNA Mus musculus
68 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 TYW5 129450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 STX5 6811
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SSR1 6745
Proximity Label-MS Homo sapiens
72 CKAP4 10970
Proximity Label-MS Homo sapiens
73 STX18 53407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 LMNA 4000
Proximity Label-MS Homo sapiens
75 ELOVL5 60481
Proximity Label-MS Homo sapiens
76 NAPG 8774
Affinity Capture-MS Homo sapiens
77 SEC61B 10952
Proximity Label-MS Homo sapiens
78 DNAJC1 64215
Proximity Label-MS Homo sapiens
79 TMEM159 57146
Affinity Capture-MS Homo sapiens
80 DNAJC16  
Proximity Label-MS Homo sapiens
81 DERL1 79139
Proximity Label-MS Homo sapiens
82 VAMP5 10791
Affinity Capture-MS Homo sapiens
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