Gene description for MGLL
Gene name monoglyceride lipase
Gene symbol MGLL
Other names/aliases HU-K5
HUK5
MAGL
MGL
Species Homo sapiens
 Database cross references - MGLL
ExoCarta ExoCarta_11343
Vesiclepedia VP_11343
Entrez Gene 11343
HGNC 17038
MIM 609699
UniProt Q99685  
 MGLL identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MGLL
Molecular Function
    lysophospholipase activity GO:0004622 TAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    monoacylglycerol lipase activity GO:0047372 IBA
    monoacylglycerol lipase activity GO:0047372 IDA
    monoacylglycerol lipase activity GO:0047372 ISS
    monoacylglycerol lipase activity GO:0047372 TAS
Biological Process
    lipid metabolic process GO:0006629 TAS
    fatty acid biosynthetic process GO:0006633 IEA
    inflammatory response GO:0006954 TAS
    regulation of signal transduction GO:0009966 ISS
    arachidonate metabolic process GO:0019369 ISS
    arachidonate metabolic process GO:0019369 TAS
    triglyceride catabolic process GO:0019433 IEA
    acylglycerol catabolic process GO:0046464 IDA
    acylglycerol catabolic process GO:0046464 ISS
    regulation of inflammatory response GO:0050727 ISS
    regulation of sensory perception of pain GO:0051930 ISS
    monoacylglycerol catabolic process GO:0052651 ISS
    regulation of endocannabinoid signaling pathway GO:2000124 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 ISS
 Experiment description of studies that identified MGLL in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MGLL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HPCAL1 3241
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NCS1 23413
Affinity Capture-MS Homo sapiens
3 ADRB2  
Two-hybrid Homo sapiens
4 SRRT 51593
Affinity Capture-MS Homo sapiens
5 USE1 55850
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 C1orf111  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HTR4  
Two-hybrid Homo sapiens
8 MGLL 11343
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
9 CHGB 1114
Two-hybrid Homo sapiens
10 LTB4R2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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