Gene description for NCS1
Gene name neuronal calcium sensor 1
Gene symbol NCS1
Other names/aliases FLUP
FREQ
Species Homo sapiens
 Database cross references - NCS1
ExoCarta ExoCarta_23413
Vesiclepedia VP_23413
Entrez Gene 23413
HGNC 3953
MIM 603315
UniProt P62166  
 NCS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NCS1
Molecular Function
    voltage-gated calcium channel activity GO:0005245 ISS
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 TAS
    protein binding GO:0005515 IPI
    calcium sensitive guanylate cyclase activator activity GO:0008048 IBA
Biological Process
    regulation of signal transduction GO:0009966 IBA
    regulation of neuron projection development GO:0010975 ISS
    calcium ion transmembrane transport GO:0070588 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IDA
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified NCS1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTAG1A  
Two-hybrid Homo sapiens
2 INTS4  
Affinity Capture-MS Homo sapiens
3 GSX1  
Affinity Capture-MS Homo sapiens
4 HMOX1 3162
Affinity Capture-MS Homo sapiens
5 NBL1 4681
Two-hybrid Homo sapiens
6 CD69  
Affinity Capture-MS Homo sapiens
7 C1QTNF2  
Two-hybrid Homo sapiens
8 CDSN 1041
Two-hybrid Homo sapiens
9 NMT1 4836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 FZD7 8324
Two-hybrid Homo sapiens
11 C3orf14  
Affinity Capture-MS Homo sapiens
12 LY6G6D  
Two-hybrid Homo sapiens
13 KIF21A 55605
Co-fractionation Homo sapiens
14 NPIPB6  
Affinity Capture-MS Homo sapiens
15 CEP89 84902
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 MGLL 11343
Affinity Capture-MS Homo sapiens
17 SPRED2 200734
Two-hybrid Homo sapiens
18 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 RNF19B  
Affinity Capture-MS Homo sapiens
21 YWHAB 7529
Affinity Capture-MS Homo sapiens
22 DRD3  
Affinity Capture-Western Homo sapiens
23 SPP1 6696
Two-hybrid Homo sapiens
24 HSFX1  
Affinity Capture-MS Homo sapiens
25 FAS 355
Proximity Label-MS Homo sapiens
26 DEFB116  
Affinity Capture-MS Homo sapiens
27 CTAG1B  
Two-hybrid Homo sapiens
28 SSX3  
Affinity Capture-MS Homo sapiens
29 BSPRY 54836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 KCND2  
Affinity Capture-Western Homo sapiens
31 HSD17B3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 SFTPA2  
Two-hybrid Homo sapiens
35 RNASEH1 246243
Two-hybrid Homo sapiens
36 PI4KB 5298
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 TK1 7083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NAF1  
Co-fractionation Homo sapiens
39 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZC3H10  
Two-hybrid Homo sapiens
41 LRRC37A2  
Affinity Capture-MS Homo sapiens
42 KDM3B  
Co-fractionation Homo sapiens
43 PRKACA 5566
Affinity Capture-MS Homo sapiens
44 FCGR3A  
Affinity Capture-MS Homo sapiens
45 SNRPB 6628
Co-fractionation Homo sapiens
46 MKRN2 23609
Affinity Capture-RNA Homo sapiens
47 YWHAZ 7534
Affinity Capture-MS Homo sapiens
48 MIEF2  
Two-hybrid Homo sapiens
49 FBXL22  
Affinity Capture-MS Homo sapiens
50 MAP3K9  
Affinity Capture-MS Homo sapiens
51 USP24 23358
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DTX2 113878
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 MEOX2  
Two-hybrid Homo sapiens
55 CNOT3 4849
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 NMT2 9397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ABHD6 57406
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SIGLEC9 27180
Two-hybrid Homo sapiens
59 YWHAG 7532
Affinity Capture-MS Homo sapiens
60 C1orf115  
Affinity Capture-MS Homo sapiens
61 PRDM4  
Two-hybrid Homo sapiens
62 CRCP  
Affinity Capture-MS Homo sapiens
63 OXER1  
Two-hybrid Homo sapiens
64 IL1RAPL1 11141
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
65 DRD2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
66 SRP68 6730
Co-fractionation Homo sapiens
67 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NCS1 is involved
No pathways found





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