Gene description for HMOX1
Gene name heme oxygenase 1
Gene symbol HMOX1
Other names/aliases HMOX1D
HO-1
HSP32
bK286B10
Species Homo sapiens
 Database cross references - HMOX1
ExoCarta ExoCarta_3162
Vesiclepedia VP_3162
Entrez Gene 3162
HGNC 5013
MIM 141250
UniProt P09601  
 HMOX1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HMOX1
Molecular Function
    heme oxygenase (decyclizing) activity GO:0004392 IBA
    heme oxygenase (decyclizing) activity GO:0004392 IDA
    heme oxygenase (decyclizing) activity GO:0004392 IMP
    structural molecule activity GO:0005198 IMP
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 ISS
    heme binding GO:0020037 IBA
    heme binding GO:0020037 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 TAS
    endothelial cell proliferation GO:0001935 TAS
    wound healing involved in inflammatory response GO:0002246 IMP
    negative regulation of leukocyte migration GO:0002686 TAS
    regulation of transcription by RNA polymerase II GO:0006357 ISS
    heme oxidation GO:0006788 IBA
    heme oxidation GO:0006788 IDA
    intracellular iron ion homeostasis GO:0006879 IDA
    intracellular iron ion homeostasis GO:0006879 IMP
    response to oxidative stress GO:0006979 IBA
    response to oxidative stress GO:0006979 IMP
    smooth muscle hyperplasia GO:0014806 TAS
    macroautophagy GO:0016236 IEA
    positive regulation of macroautophagy GO:0016239 IEA
    negative regulation of macroautophagy GO:0016242 IEA
    positive regulation of chemokine production GO:0032722 TAS
    erythrocyte homeostasis GO:0034101 IMP
    low-density lipoprotein particle clearance GO:0034383 TAS
    cellular response to heat GO:0034605 IMP
    response to nicotine GO:0035094 IDA
    intracellular signal transduction GO:0035556 TAS
    heme catabolic process GO:0042167 IBA
    heme catabolic process GO:0042167 IDA
    response to hydrogen peroxide GO:0042542 ISS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    regulation of angiogenesis GO:0045765 TAS
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    multicellular organismal-level iron ion homeostasis GO:0060586 IMP
    cellular response to arsenic-containing substance GO:0071243 IEA
    cellular response to cadmium ion GO:0071276 IEA
    cellular response to cisplatin GO:0072719 IEA
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IGI
    negative regulation of ferroptosis GO:0110076 IMP
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:1903589 IGI
    epithelial cell apoptotic process GO:1904019 IEA
    positive regulation of epithelial cell apoptotic process GO:1904037 IEA
Subcellular Localization
    extracellular space GO:0005615 TAS
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    mitochondrial outer membrane GO:0005741 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IMP
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified HMOX1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HMOX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
2 HMOX1 3162
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
3 ZSCAN20  
Affinity Capture-MS Homo sapiens
4 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 UFD1L 7353
Affinity Capture-Western Homo sapiens
6 GPR17 2840
Affinity Capture-MS Homo sapiens
7 TMEM106A  
Affinity Capture-MS Homo sapiens
8 CPSF1 29894
Protein-RNA Homo sapiens
9 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
10 SOX2  
Affinity Capture-MS Homo sapiens
11 POT1  
Two-hybrid Homo sapiens
12 PIGT 51604
Affinity Capture-MS Homo sapiens
13 AGPAT1 10554
Affinity Capture-MS Homo sapiens
14 RNF181  
Affinity Capture-Western Homo sapiens
15 UPK1A 11045
Affinity Capture-MS Homo sapiens
16 CCDC155  
Two-hybrid Homo sapiens
17 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RAMP3  
Affinity Capture-MS Homo sapiens
19 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HPCAL1 3241
Affinity Capture-MS Homo sapiens
21 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ADAM33 80332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PPID 5481
Cross-Linking-MS (XL-MS) Homo sapiens
24 SLC39A8 64116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EDEM1  
Affinity Capture-MS Homo sapiens
26 BSCL2  
Affinity Capture-MS Homo sapiens
27 PTGER4  
Two-hybrid Homo sapiens
28 TSPAN15 23555
Affinity Capture-MS Homo sapiens
29 PPAPDC1A  
Affinity Capture-MS Homo sapiens
30 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 C1orf35  
Affinity Capture-MS Homo sapiens
32 NIPAL1  
Affinity Capture-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 HSD17B1  
Affinity Capture-MS Homo sapiens
35 GJA8  
Affinity Capture-MS Homo sapiens
36 PTDSS2 81490
Affinity Capture-MS Homo sapiens
37 SSBP2  
Affinity Capture-MS Homo sapiens
38 SYVN1 84447
Affinity Capture-MS Homo sapiens
39 MBNL1 4154
Affinity Capture-MS Homo sapiens
40 TUT1  
Protein-RNA Homo sapiens
41 CERS3  
Affinity Capture-MS Homo sapiens
42 HCN1  
Affinity Capture-MS Homo sapiens
43 RNF115  
Affinity Capture-MS Homo sapiens
44 PTDSS1 9791
Affinity Capture-MS Homo sapiens
45 LRRC27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NCS1 23413
Affinity Capture-MS Homo sapiens
47 LPCAT2 54947
Affinity Capture-MS Homo sapiens
48 CHRNB2  
Affinity Capture-MS Homo sapiens
49 TERF1 7013
Two-hybrid Homo sapiens
50 TEX264 51368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CHRNA9  
Affinity Capture-MS Homo sapiens
52 CCR1  
Affinity Capture-MS Homo sapiens
53 TNPO2 30000
Affinity Capture-MS Homo sapiens
54 PSMD2 5708
Affinity Capture-Western Homo sapiens
55 SARAF  
Affinity Capture-MS Homo sapiens
56 SMO 6608
Two-hybrid Homo sapiens
57 PINK1  
Affinity Capture-MS Homo sapiens
58 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 GOT1 2805
Affinity Capture-MS Homo sapiens
60 STIM2 57620
Affinity Capture-MS Homo sapiens
61 SSUH2  
Affinity Capture-MS Homo sapiens
62 CREB3  
Two-hybrid Homo sapiens
63 B3GAT3 26229
Affinity Capture-MS Homo sapiens
64 ADGRE5 976
Affinity Capture-MS Homo sapiens
65 OR2A4 79541
Affinity Capture-MS Homo sapiens
66 CDC20B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PCDHAC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC37A4 2542
Affinity Capture-MS Homo sapiens
69 ASL 435
Two-hybrid Homo sapiens
70 GUCY1A3  
Affinity Capture-MS Homo sapiens
71 STX17 55014
Affinity Capture-MS Homo sapiens
72 TAP1 6890
Affinity Capture-MS Homo sapiens
73 PIGS 94005
Affinity Capture-MS Homo sapiens
74 UGT8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PHF11 51131
Affinity Capture-MS Homo sapiens
76 DERL1 79139
Affinity Capture-MS Homo sapiens
77 CCDC47 57003
Affinity Capture-MS Homo sapiens
78 GBAS 2631
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HMOX1 is involved
PathwayEvidenceSource
Cell recruitment (pro-inflammatory response) IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular response to chemical stress TAS Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress IEA Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Cytoprotection by HMOX1 IEA Reactome
Cytoprotection by HMOX1 TAS Reactome
Disease IEA Reactome
Heme degradation TAS Reactome
Heme signaling TAS Reactome
Immune System IEA Reactome
Immune System TAS Reactome
Infectious disease IEA Reactome
Inflammasomes IEA Reactome
Innate Immune System IEA Reactome
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane TAS Reactome
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane IEA Reactome
Interleukin-4 and Interleukin-13 signaling TAS Reactome
Iron uptake and transport TAS Reactome
KEAP1-NFE2L2 pathway TAS Reactome
Leishmania infection IEA Reactome
Metabolism TAS Reactome
Metabolism of porphyrins TAS Reactome
NFE2L2 regulating anti-oxidant/detoxification enzymes TAS Reactome
Nuclear events mediated by NFE2L2 TAS Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways IEA Reactome
Parasitic Infection Pathways IEA Reactome
Protein localization TAS Reactome
Protein localization IEA Reactome
Purinergic signaling in leishmaniasis infection IEA Reactome
Regulation of HMOX1 expression and activity TAS Reactome
Signaling by Interleukins TAS Reactome
The NLRP3 inflammasome IEA Reactome
Transport of small molecules TAS Reactome





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