Gene description for ADAM33
Gene name ADAM metallopeptidase domain 33
Gene symbol ADAM33
Other names/aliases C20orf153
DJ964F7.1
Species Homo sapiens
 Database cross references - ADAM33
ExoCarta ExoCarta_80332
Vesiclepedia VP_80332
Entrez Gene 80332
HGNC 15478
MIM 607114
UniProt Q9BZ11  
 ADAM33 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ADAM33
Molecular Function
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 NAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 NAS
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 NAS
Subcellular Localization
    membrane GO:0016020 NAS
 Experiment description of studies that identified ADAM33 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ADAM33
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCB 6443
Two-hybrid Homo sapiens
2 ADAM10 102
Affinity Capture-MS Homo sapiens
3 CLDN2 9075
Two-hybrid Homo sapiens
4 HMOX1 3162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TOR3A 64222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HIBADH 11112
Two-hybrid Homo sapiens
7 NOTCH3 4854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TTC17 55761
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SLC30A2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 TMBIM6 7009
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HLA-B 3106
Affinity Capture-MS Homo sapiens
12 NETO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LRP12 29967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CISD3 284106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM39B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMEM106C 79022
Two-hybrid Homo sapiens
17 AMIGO1  
Affinity Capture-MS Homo sapiens
18 LSR 51599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KIR3DL3  
Two-hybrid Homo sapiens
20 SEC11A 23478
Two-hybrid Homo sapiens
21 ATXN7  
Two-hybrid Homo sapiens
22 TNFSF14  
Two-hybrid Homo sapiens
23 ASGR2  
Two-hybrid Homo sapiens
24 TMEM14B  
Two-hybrid Homo sapiens
25 TYW1  
Affinity Capture-MS Homo sapiens
26 CLEC7A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 FCGR2A 2212
Two-hybrid Homo sapiens
28 CLSTN1 22883
Affinity Capture-MS Homo sapiens
29 ADAM15 8751
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TMEM67 91147
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM39A  
Affinity Capture-MS Homo sapiens
32 GALNT12 79695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 EPGN  
Two-hybrid Homo sapiens
34 EGFL7 51162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SDF2L1 23753
Affinity Capture-MS Homo sapiens
36 SOAT1 6646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SLC35E2 9906
Two-hybrid Homo sapiens
38 SPINT1 6692
Two-hybrid Homo sapiens
39 BTNL9  
Two-hybrid Homo sapiens
40 ZDHHC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MANEAL 149175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 LRP6 4040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SLC27A3 11000
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NCR1  
Two-hybrid Homo sapiens
46 KCNJ8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PDF  
Affinity Capture-MS Homo sapiens
48 GGT6  
Two-hybrid Homo sapiens
49 MUC1 4582
Two-hybrid Homo sapiens
50 SPCS1 28972
Affinity Capture-MS Homo sapiens
51 UBQLN4 56893
Two-hybrid Homo sapiens
52 SLC22A23  
Two-hybrid Homo sapiens
53 ADAMTS2 9509
Affinity Capture-MS Homo sapiens
54 OPRM1 4988
Two-hybrid Homo sapiens
55 DHFRL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SEMA4F  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 VMA21  
Two-hybrid Homo sapiens
58 TMEM237 65062
Two-hybrid Homo sapiens
59 ITGB5 3693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CD3E 916
Two-hybrid Homo sapiens
61 DGCR2 9993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ANKRD46 157567
Affinity Capture-MS Homo sapiens
63 HLA-A 3105
Affinity Capture-MS Homo sapiens
64 TMEM131  
Affinity Capture-MS Homo sapiens
65 STOML3 161003
Two-hybrid Homo sapiens
66 FSTL1 11167
Affinity Capture-MS Homo sapiens
67 ASB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PIGT 51604
Affinity Capture-MS Homo sapiens
69 CREB3L1  
Two-hybrid Homo sapiens
70 LRP5 4041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 AQP9  
Two-hybrid Homo sapiens
73 FZD2 2535
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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