Gene description for SPCS1
Gene name signal peptidase complex subunit 1 homolog (S. cerevisiae)
Gene symbol SPCS1
Other names/aliases HSPC033
SPC1
SPC12
YJR010C-A
Species Homo sapiens
 Database cross references - SPCS1
ExoCarta ExoCarta_28972
Vesiclepedia VP_28972
Entrez Gene 28972
HGNC 23401
MIM 610358
UniProt Q9Y6A9  
 SPCS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SPCS1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal peptide processing GO:0006465 IBA
    signal peptide processing GO:0006465 IDA
    proteolysis GO:0006508 TAS
    virion assembly GO:0019068 IMP
    viral protein processing GO:0019082 IMP
    protein targeting to ER GO:0045047 IBA
Subcellular Localization
    signal peptidase complex GO:0005787 IBA
    signal peptidase complex GO:0005787 IPI
    signal peptidase complex GO:0005787 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified SPCS1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPCS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXL14  
Affinity Capture-MS Homo sapiens
2 SPATS2  
Affinity Capture-MS Homo sapiens
3 UTP18  
Affinity Capture-MS Homo sapiens
4 SLC29A1 2030
Affinity Capture-MS Homo sapiens
5 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GMNN  
Affinity Capture-MS Homo sapiens
7 MBLAC2 153364
Affinity Capture-MS Homo sapiens
8 VAPA 9218
Affinity Capture-MS Homo sapiens
9 TMED4 222068
Affinity Capture-MS Homo sapiens
10 PRPF38A 84950
Affinity Capture-MS Homo sapiens
11 GPC1 2817
Affinity Capture-MS Homo sapiens
12 ALPL 249
Affinity Capture-MS Homo sapiens
13 ATP12A 479
Affinity Capture-MS Homo sapiens
14 ESF1 51575
Affinity Capture-MS Homo sapiens
15 KLRK1  
Affinity Capture-MS Homo sapiens
16 SRFBP1  
Affinity Capture-MS Homo sapiens
17 BUD13  
Affinity Capture-MS Homo sapiens
18 NAALAD2 10003
Affinity Capture-MS Homo sapiens
19 TOMM22 56993
Affinity Capture-MS Homo sapiens
20 TCEB3 6924
Affinity Capture-MS Homo sapiens
21 TM2D3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FIS1 51024
Two-hybrid Homo sapiens
23 GTF2F2 2963
Affinity Capture-MS Homo sapiens
24 RPRM  
Two-hybrid Homo sapiens
25 PRPF38B 55119
Affinity Capture-MS Homo sapiens
26 LENG1  
Affinity Capture-MS Homo sapiens
27 ZAK 51776
Affinity Capture-MS Homo sapiens
28 PVRIG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RIOK2 55781
Affinity Capture-MS Homo sapiens
30 ZGPAT  
Affinity Capture-MS Homo sapiens
31 LSG1  
Affinity Capture-MS Homo sapiens
32 NRN1  
Affinity Capture-MS Homo sapiens
33 GTF2F1 2962
Affinity Capture-MS Homo sapiens
34 SLC25A46 91137
Two-hybrid Homo sapiens
35 SEC11C 90701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MTDH 92140
Affinity Capture-MS Homo sapiens
37 PRTN3 5657
Affinity Capture-MS Homo sapiens
38 ATP13A3 79572
Affinity Capture-MS Homo sapiens
39 ERBB2 2064
Affinity Capture-MS Homo sapiens
40 LMAN1L  
Affinity Capture-MS Homo sapiens
41 SSMEM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 GRIN3B  
Affinity Capture-MS Homo sapiens
43 UNK  
Affinity Capture-RNA Homo sapiens
44 RBMXL1  
Affinity Capture-MS Homo sapiens
45 MOXD1 26002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TM2D2 83877
Affinity Capture-MS Homo sapiens
47 WNT6  
Affinity Capture-MS Homo sapiens
48 RBM6 10180
Affinity Capture-MS Homo sapiens
49 COPE 11316
Affinity Capture-MS Homo sapiens
50 BTN2A3P  
Affinity Capture-MS Homo sapiens
51 IWS1  
Affinity Capture-MS Homo sapiens
52 CCDC51 79714
Affinity Capture-MS Homo sapiens
53 UBXN8  
Two-hybrid Homo sapiens
54 CLCC1 23155
Affinity Capture-MS Homo sapiens
55 SAFB2 9667
Affinity Capture-MS Homo sapiens
56 PCDHGA3  
Affinity Capture-MS Homo sapiens
57 DHX16 8449
Affinity Capture-MS Homo sapiens
58 SLC28A2  
Affinity Capture-MS Homo sapiens
59 SNRNP27  
Affinity Capture-MS Homo sapiens
60 TSPAN31 6302
Affinity Capture-MS Homo sapiens
61 PRSS21  
Affinity Capture-MS Homo sapiens
62 NUMB 8650
Affinity Capture-MS Homo sapiens
63 CCDC47 57003
Affinity Capture-MS Homo sapiens
64 DNTTIP2  
Affinity Capture-MS Homo sapiens
65 GTPBP4 23560
Affinity Capture-MS Homo sapiens
66 ST3GAL3  
Affinity Capture-MS Homo sapiens
67 HABP4  
Affinity Capture-MS Homo sapiens
68 HSPA5 3309
Affinity Capture-MS Homo sapiens
69 AQP2 359
Two-hybrid Homo sapiens
70 EFNA4  
Affinity Capture-MS Homo sapiens
71 GPC4 2239
Affinity Capture-MS Homo sapiens
72 HYAL4  
Affinity Capture-MS Homo sapiens
73 TTYH1  
Affinity Capture-MS Homo sapiens
74 CANX 821
Affinity Capture-MS Homo sapiens
75 GABRA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 RTN4R 65078
Affinity Capture-MS Homo sapiens
77 CRELD1 78987
Affinity Capture-MS Homo sapiens
78 CYB5R3 1727
Co-fractionation Homo sapiens
79 DCAF13 25879
Affinity Capture-MS Homo sapiens
80 IGF1  
Affinity Capture-MS Homo sapiens
81 FXYD6-FXYD2  
Two-hybrid Homo sapiens
82 RBM27  
Affinity Capture-MS Homo sapiens
83 TMEM74  
Two-hybrid Homo sapiens
84 SLTM 79811
Affinity Capture-MS Homo sapiens
85 RRP36 88745
Affinity Capture-MS Homo sapiens
86 RP9  
Affinity Capture-MS Homo sapiens
87 TREM1 54210
Affinity Capture-MS Homo sapiens
88 TJP2 9414
Affinity Capture-MS Homo sapiens
89 RFT1 91869
Affinity Capture-MS Homo sapiens
90 PRRC2A 7916
Affinity Capture-MS Homo sapiens
91 NCLN 56926
Affinity Capture-MS Homo sapiens
92 C12orf43  
Affinity Capture-MS Homo sapiens
93 PKD2 5311
Affinity Capture-MS Homo sapiens
94 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 CTSF 8722
Affinity Capture-MS Homo sapiens
96 PLXNB2 23654
Affinity Capture-MS Homo sapiens
97 SLC9A6 10479
Affinity Capture-MS Homo sapiens
98 CACNA2D3  
Affinity Capture-MS Homo sapiens
99 DDX46 9879
Affinity Capture-MS Homo sapiens
100 DMXL2  
Affinity Capture-MS Homo sapiens
101 ADAM30 11085
Affinity Capture-MS Homo sapiens
102 SLC19A2 10560
Affinity Capture-MS Homo sapiens
103 SPCS3 60559
Affinity Capture-MS Homo sapiens
104 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 VNN2  
Affinity Capture-MS Homo sapiens
106 TAF1A  
Affinity Capture-MS Homo sapiens
107 TP53 7157
Affinity Capture-MS Homo sapiens
108 WBP11  
Affinity Capture-MS Homo sapiens
109 SEC11A 23478
Affinity Capture-MS Homo sapiens
110 ADAM33 80332
Affinity Capture-MS Homo sapiens
111 EMC4 51234
Affinity Capture-MS Homo sapiens
112 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 AGPAT3 56894
Affinity Capture-MS Homo sapiens
114 TRIP12 9320
Affinity Capture-MS Homo sapiens
115 OTUD4  
Affinity Capture-MS Homo sapiens
116 MGST1 4257
Co-fractionation Homo sapiens
117 ARSG 22901
Affinity Capture-MS Homo sapiens
118 YIPF2 78992
Two-hybrid Homo sapiens
119 MAP1A 4130
Affinity Capture-MS Homo sapiens
120 PTRF 284119
Affinity Capture-MS Homo sapiens
121 ARSK  
Affinity Capture-MS Homo sapiens
122 NEMF 9147
Affinity Capture-MS Homo sapiens
123 SPCS2 9789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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