Gene description for NAALAD2
Gene name N-acetylated alpha-linked acidic dipeptidase 2
Gene symbol NAALAD2
Other names/aliases GCPIII
GPCIII
NAADALASE2
NAALADASE2
Species Homo sapiens
 Database cross references - NAALAD2
ExoCarta ExoCarta_10003
Vesiclepedia VP_10003
Entrez Gene 10003
HGNC 14526
MIM 611636
UniProt Q9Y3Q0  
 NAALAD2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for NAALAD2
Molecular Function
    carboxypeptidase activity GO:0004180 IBA
    carboxypeptidase activity GO:0004180 NAS
    metallocarboxypeptidase activity GO:0004181 IEA
    protein binding GO:0005515 IPI
    serine-type peptidase activity GO:0008236 NAS
    dipeptidyl-peptidase activity GO:0008239 NAS
    dipeptidase activity GO:0016805 NAS
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 NAS
 Experiment description of studies that identified NAALAD2 in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for NAALAD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCGB1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CLEC12B  
Affinity Capture-MS Homo sapiens
3 FOLH1 2346
Affinity Capture-MS Homo sapiens
4 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NAALADL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SPCS1 28972
Affinity Capture-MS Homo sapiens
7 GGT7 2686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SCGB2A2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NAALAD2 is involved
PathwayEvidenceSource
Aspartate and asparagine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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