Gene description for FOLH1
Gene name folate hydrolase (prostate-specific membrane antigen) 1
Gene symbol FOLH1
Other names/aliases FGCP
FOLH
GCP2
GCPII
NAALAD1
NAALAdase
PSM
PSMA
mGCP
Species Homo sapiens
 Database cross references - FOLH1
ExoCarta ExoCarta_2346
Entrez Gene 2346
HGNC 3788
MIM 600934
UniProt Q04609  
 FOLH1 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 22723089    
Saliva 19199708    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FOLH1
Molecular Function
    metallopeptidase activity GO:0008237 IEA
    carboxypeptidase activity GO:0004180 IEA
    peptidase activity GO:0008233 NAS
    metal ion binding GO:0046872 IEA
    dipeptidase activity GO:0016805 IEA
Biological Process
    folic acid-containing compound metabolic process GO:0006760 IEA
    proteolysis GO:0006508 NAS
Subcellular Localization
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified FOLH1 in exosomes
1
Experiment ID 144
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90|RAB5
EV Cytosolic markers
CD9
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FOLH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which FOLH1 is involved
No pathways found





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