Gene description for LRP5
Gene name low density lipoprotein receptor-related protein 5
Gene symbol LRP5
Other names/aliases BMND1
EVR1
EVR4
HBM
LR3
LRP-5
LRP7
OPPG
OPS
OPTA1
VBCH2
Species Homo sapiens
 Database cross references - LRP5
ExoCarta ExoCarta_4041
Vesiclepedia VP_4041
Entrez Gene 4041
HGNC 6697
MIM 603506
UniProt O75197  
 LRP5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for LRP5
Molecular Function
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 IPI
    coreceptor activity GO:0015026 NAS
    Wnt-protein binding GO:0017147 IPI
    Wnt-protein binding GO:0017147 ISS
    Wnt-protein binding GO:0017147 TAS
    toxin transmembrane transporter activity GO:0019534 IMP
    Wnt receptor activity GO:0042813 IDA
    Wnt receptor activity GO:0042813 ISS
Biological Process
    gastrulation with mouth forming second GO:0001702 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IMP
    osteoblast development GO:0002076 IEA
    glucose catabolic process GO:0006007 IMP
    amino acid transport GO:0006865 IEA
    endocytosis GO:0006897 IEA
    nervous system development GO:0007399 IBA
    mesodermal cell migration GO:0008078 IEA
    cholesterol metabolic process GO:0008203 IEA
    regulation of blood pressure GO:0008217 IMP
    positive regulation of cell population proliferation GO:0008284 IDA
    anterior/posterior pattern specification GO:0009952 IEA
    gene expression GO:0010467 IEA
    osteoblast proliferation GO:0033687 IEA
    positive regulation of osteoblast proliferation GO:0033690 IEA
    somatic stem cell population maintenance GO:0035019 IEA
    extracellular matrix-cell signaling GO:0035426 IEA
    cell migration involved in gastrulation GO:0042074 IEA
    cholesterol homeostasis GO:0042632 IMP
    embryonic digit morphogenesis GO:0042733 IEA
    regulation of apoptotic process GO:0042981 IEA
    response to peptide hormone GO:0043434 IEA
    positive regulation of fat cell differentiation GO:0045600 IMP
    negative regulation of osteoblast differentiation GO:0045668 IMP
    positive regulation of osteoblast differentiation GO:0045669 ISS
    positive regulation of mitotic nuclear division GO:0045840 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    bone remodeling GO:0046849 IEA
    bone marrow development GO:0048539 IMP
    anatomical structure regression GO:0060033 IEA
    retina morphogenesis in camera-type eye GO:0060042 IMP
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IGI
    canonical Wnt signaling pathway GO:0060070 IMP
    bone morphogenesis GO:0060349 IMP
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    adipose tissue development GO:0060612 IMP
    cell-cell signaling involved in mammary gland development GO:0060764 IEA
    establishment of blood-brain barrier GO:0060856 IEA
    regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061178 IEA
    retinal blood vessel morphogenesis GO:0061304 IMP
    negative regulation of protein serine/threonine kinase activity GO:0071901 IMP
    cell-cell adhesion GO:0098609 IEA
    Norrin signaling pathway GO:0110135 IDA
    apoptotic process involved in blood vessel morphogenesis GO:1902262 IEA
    establishment of blood-retinal barrier GO:1990963 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IDA
    Wnt-Frizzled-LRP5/6 complex GO:1990851 TAS
    Wnt signalosome GO:1990909 NAS
 Experiment description of studies that identified LRP5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for LRP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ADAM30 11085
Affinity Capture-MS Homo sapiens
3 B3GALT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GPIHBP1  
Affinity Capture-MS Homo sapiens
5 BAGE2  
Affinity Capture-MS Homo sapiens
6 LIPG 9388
Affinity Capture-MS Homo sapiens
7 GPATCH2  
Affinity Capture-MS Homo sapiens
8 DKK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RBM45  
Affinity Capture-MS Homo sapiens
10 ETV6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SERPINA12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCL17  
Affinity Capture-MS Homo sapiens
13 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
14 TMEM87A 25963
Affinity Capture-MS Homo sapiens
15 LIPH 200879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 HLA-G 3135
Affinity Capture-MS Homo sapiens
17 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KLK5 25818
Affinity Capture-MS Homo sapiens
19 NXPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DNASE2B  
Affinity Capture-MS Homo sapiens
23 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ADAM33 80332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CTNNB1 1499
Affinity Capture-Western Homo sapiens
26 STMN1 3925
Cross-Linking-MS (XL-MS) Homo sapiens
27 CFC1  
Affinity Capture-MS Homo sapiens
28 KLK15  
Affinity Capture-MS Homo sapiens
29 ADAMTS4  
Affinity Capture-MS Homo sapiens
30 PRG2  
Affinity Capture-MS Homo sapiens
31 TRAF6 7189
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
32 PI15  
Affinity Capture-MS Homo sapiens
33 NRSN1  
Affinity Capture-MS Homo sapiens
34 ST8SIA5  
Affinity Capture-MS Homo sapiens
35 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 FZD8 8325
Reconstituted Complex Homo sapiens
37 LRRC4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 LYZL1  
Affinity Capture-MS Homo sapiens
40 WNT10B 7480
Affinity Capture-MS Homo sapiens
41 OVOL2  
Affinity Capture-MS Homo sapiens
42 AXIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 ELOVL5 60481
Proximity Label-MS Homo sapiens
44 DKK1 22943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
45 NMS  
Affinity Capture-MS Homo sapiens
46 FAM3B 54097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ZNF383  
Affinity Capture-MS Homo sapiens
48 DNMT3B  
Affinity Capture-MS Homo sapiens
49 ST3GAL4 6484
Affinity Capture-MS Homo sapiens
50 B3GAT1  
Proximity Label-MS Homo sapiens
51 SIRPD  
Affinity Capture-MS Homo sapiens
52 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SDF2L1 23753
Affinity Capture-MS Homo sapiens
54 SMAD9  
Two-hybrid Homo sapiens
55 RNF10  
Affinity Capture-MS Homo sapiens
56 PRSS37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CRISP2  
Affinity Capture-MS Homo sapiens
58 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 NCR3  
Affinity Capture-MS Homo sapiens
60 DEFB135  
Affinity Capture-MS Homo sapiens
61 DNAJB9 4189
Affinity Capture-MS Homo sapiens
62 CLEC2B  
Affinity Capture-MS Homo sapiens
63 LGI1  
Affinity Capture-MS Homo sapiens
64 CLPSL1  
Affinity Capture-MS Homo sapiens
65 LYPD1  
Affinity Capture-MS Homo sapiens
66 MARCH4  
Affinity Capture-MS Homo sapiens
67 KLK3  
Affinity Capture-MS Homo sapiens
68 RASD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 TGIF2LY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 WNT10A  
Affinity Capture-MS Homo sapiens
71 EDN3  
Affinity Capture-MS Homo sapiens
72 CHST9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PODN 127435
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 CCDC140  
Affinity Capture-MS Homo sapiens
75 SOST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CHSY3 337876
Affinity Capture-MS Homo sapiens
77 TIMP3 7078
Affinity Capture-MS Homo sapiens
78 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 F9 2158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PARP16 54956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 DKK2  
Affinity Capture-MS Homo sapiens
82 IGFL3  
Affinity Capture-MS Homo sapiens
83 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 IL5RA  
Affinity Capture-MS Homo sapiens
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