Gene description for FZD8
Gene name frizzled class receptor 8
Gene symbol FZD8
Other names/aliases FZ-8
hFZ8
Species Homo sapiens
 Database cross references - FZD8
ExoCarta ExoCarta_8325
Vesiclepedia VP_8325
Entrez Gene 8325
HGNC 4046
MIM 606146
UniProt Q9H461  
 FZD8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for FZD8
Molecular Function
    G protein-coupled receptor activity GO:0004930 IEA
    signaling receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    Wnt-protein binding GO:0017147 IBA
    PDZ domain binding GO:0030165 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    Wnt receptor activity GO:0042813 IBA
    Wnt receptor activity GO:0042813 IDA
    Wnt receptor activity GO:0042813 TAS
Biological Process
    angiogenesis GO:0001525 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    neuron differentiation GO:0030182 ISS
    T cell differentiation in thymus GO:0033077 IEA
    non-canonical Wnt signaling pathway GO:0035567 IBA
    canonical Wnt signaling pathway GO:0060070 IBA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 TAS
    neuronal dense core vesicle GO:0098992 IEA
    Wnt-Frizzled-LRP5/6 complex GO:1990851 IEA
 Experiment description of studies that identified FZD8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FZD8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KCNK18  
Affinity Capture-MS Homo sapiens
2 FSHR  
Affinity Capture-MS Homo sapiens
3 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DVL3 1857
Affinity Capture-Western Homo sapiens
5 RNF43  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 SCN4A  
Affinity Capture-MS Homo sapiens
7 WNT1  
Reconstituted Complex Homo sapiens
8 BRCA2 675
Affinity Capture-MS Homo sapiens
9 PLD3 23646
Affinity Capture-MS Homo sapiens
10 TSPAN15 23555
Affinity Capture-MS Homo sapiens
11 UPK1A 11045
Affinity Capture-MS Homo sapiens
12 OR1D4  
Affinity Capture-MS Homo sapiens
13 LRP6 4040
Reconstituted Complex Homo sapiens
14 LRP5 4041
Reconstituted Complex Homo sapiens
15 SIDT2 51092
Affinity Capture-MS Homo sapiens
16 EPN1 29924
Affinity Capture-Western Homo sapiens
17 TMEM169  
Affinity Capture-MS Homo sapiens
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