Gene description for LRP6
Gene name low density lipoprotein receptor-related protein 6
Gene symbol LRP6
Other names/aliases ADCAD2
Species Homo sapiens
 Database cross references - LRP6
ExoCarta ExoCarta_4040
Vesiclepedia VP_4040
Entrez Gene 4040
HGNC 6698
MIM 603507
UniProt O75581  
 LRP6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for LRP6
Molecular Function
    protein homodimerization activity GO:0042803 IPI
    frizzled binding GO:0005109 IPI
    toxin transporter activity GO:0019534 IMP
    Wnt-protein binding GO:0017147 IPI
    receptor binding GO:0005102 IPI
    coreceptor activity involved in Wnt signaling pathway GO:0071936 IDA
    kinase inhibitor activity GO:0019210 IMP
    identical protein binding GO:0042802 IPI
    low-density lipoprotein receptor activity GO:0005041 IDA
    Wnt-activated receptor activity GO:0042813 IBA
    protein binding GO:0005515 IPI
    apolipoprotein binding GO:0034185 IBA
Biological Process
    embryonic hindlimb morphogenesis GO:0035116 IEA
    cerebral cortex cell migration GO:0021795 IEA
    heart looping GO:0001947 IEA
    convergent extension GO:0060026 IBA
    dopaminergic neuron differentiation GO:0071542 IEA
    external genitalia morphogenesis GO:0035261 IBA
    positive regulation of apoptotic process GO:0043065 IEA
    canonical Wnt signaling pathway GO:0060070 IMP
    canonical Wnt signaling pathway involved in neural crest cell differentiation GO:0044335 IC
    neural tube closure GO:0001843 IBA
    embryonic forelimb morphogenesis GO:0035115 IEA
    thalamus development GO:0021794 IBA
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    skeletal system morphogenesis GO:0048705 IEA
    face morphogenesis GO:0060325 IBA
    cerebral cortex development GO:0021987 IBA
    negative regulation of planar cell polarity pathway involved in ventricular septum morphogenesis GO:2000149 IEA
    post-anal tail morphogenesis GO:0036342 IEA
    forebrain radial glial cell differentiation GO:0021861 IEA
    mammary placode formation GO:0060596 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    Wnt signaling pathway involved in forebrain neuroblast division GO:0021874 IEA
    embryonic camera-type eye morphogenesis GO:0048596 IEA
    embryonic pattern specification GO:0009880 IBA
    primitive streak formation GO:0090009 IBA
    negative regulation of protein kinase activity GO:0006469 IMP
    Wnt signaling pathway GO:0016055 IMP
    receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport GO:0090118 IBA
    Wnt signaling pathway involved in dorsal/ventral axis specification GO:0044332 IDA
    negative regulation of planar cell polarity pathway involved in neural tube closure GO:2000168 IEA
    negative regulation of planar cell polarity pathway involved in pericardium morphogenesis GO:2000166 IEA
    canonical Wnt signaling pathway involved in cardiac neural crest cell differentiation involved in heart development GO:0061310 IEA
    axis elongation involved in somitogenesis GO:0090245 IBA
    negative regulation of fat cell differentiation GO:0045599 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    trachea cartilage morphogenesis GO:0060535 IBA
    positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification GO:2000055 IDA
    regulation of cell development GO:0060284 IEA
    negative regulation of planar cell polarity pathway involved in cardiac right atrium morphogenesis GO:2000162 IEA
    neural crest cell differentiation GO:0014033 IDA
    negative regulation of planar cell polarity pathway involved in outflow tract morphogenesis GO:2000164 IEA
    bone remodeling GO:0046849 IEA
    positive regulation of bone resorption GO:0045780 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    positive regulation of cell cycle GO:0045787 IMP
    response to peptide hormone GO:0043434 IEA
    palate development GO:0060021 IBA
    canonical Wnt signaling pathway involved in regulation of cell proliferation GO:0044340 IC
    positive regulation of ossification GO:0045778 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IBA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    cerebellum morphogenesis GO:0021587 IBA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    midbrain development GO:0030901 IBA
    neural crest formation GO:0014029 IDA
    Wnt signaling pathway involved in somitogenesis GO:0090244 IBA
    pericardium morphogenesis GO:0003344 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    single organismal cell-cell adhesion GO:0016337 IEA
    negative regulation of smooth muscle cell apoptotic process GO:0034392 IMP
    midbrain-hindbrain boundary development GO:0030917 IBA
    toxin transport GO:1901998 IMP
    cell migration involved in gastrulation GO:0042074 IEA
    canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation GO:0061324 IEA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    formation of radial glial scaffolds GO:0021943 IEA
    synaptic transmission GO:0007268 IBA
    negative regulation of planar cell polarity pathway involved in cardiac muscle tissue morphogenesis GO:2000151 IEA
    negative regulation of protein phosphorylation GO:0001933 IMP
    response to folic acid GO:0051593 IEA
    cellular response to cholesterol GO:0071397 IMP
    embryonic retina morphogenesis in camera-type eye GO:0060059 IBA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    embryonic digit morphogenesis GO:0042733 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    receptor complex GO:0043235 IBA
    cytosol GO:0005829 IEA
    neuronal cell body GO:0043025 IBA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    caveola GO:0005901 IDA
    early endosome membrane GO:0031901 TAS
    synapse GO:0045202 IBA
    integral component of membrane GO:0016021 IEA
    early endosome GO:0005769 IDA
    cytoplasmic vesicle GO:0031410 IDA
    Golgi apparatus GO:0005794 IDA
    endoplasmic reticulum GO:0005783 IEA
 Experiment description of studies that identified LRP6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for LRP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DKK1 22943
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 MESDC2 23184
Affinity Capture-Western Homo sapiens
3 WNT1  
Affinity Capture-Western Homo sapiens
4 DKK2  
Invivo Homo sapiens
5 FZD8 8325
Affinity Capture-Western Homo sapiens
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