Gene description for FZD5
Gene name frizzled class receptor 5
Gene symbol FZD5
Other names/aliases C2orf31
HFZ5
Species Homo sapiens
 Database cross references - FZD5
ExoCarta ExoCarta_7855
Entrez Gene 7855
HGNC 4043
MIM 601723
UniProt Q13467  
 FZD5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for FZD5
Molecular Function
    Wnt-activated receptor activity GO:0042813 IC
    protein binding GO:0005515 IPI
    Wnt-protein binding GO:0017147 IPI
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    G-protein coupled receptor activity GO:0004930 IBA
Biological Process
    embryonic camera-type eye development GO:0031076 IEA
    regulation of bicellular tight junction assembly GO:2000810 IEA
    angiogenesis GO:0001525 IEA
    chorionic trophoblast cell differentiation GO:0060718 IEA
    Spemann organizer formation GO:0060061 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    embryonic axis specification GO:0000578 IDA
    positive regulation of JUN kinase activity GO:0043507 IEA
    non-canonical Wnt signaling pathway GO:0035567 IBA
    Wnt signaling pathway involved in dorsal/ventral axis specification GO:0044332 IDA
    canonical Wnt signaling pathway GO:0060070 IMP
    syncytiotrophoblast cell differentiation involved in labyrinthine layer development GO:0060715 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    positive regulation of T cell cytokine production GO:0002726 IDA
    negative regulation of cell proliferation GO:0008285 IEA
    cell maturation GO:0048469 IEA
    post-embryonic camera-type eye development GO:0031077 IEA
    regulation of chorionic trophoblast cell proliferation GO:1901382 IEA
    branching involved in labyrinthine layer morphogenesis GO:0060670 IEA
    cellular response to molecule of bacterial origin GO:0071219 IDA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    apoptotic process involved in morphogenesis GO:0060561 IEA
    regulation of mitochondrion degradation GO:1903146 IMP
    neuron differentiation GO:0030182 ISS
    T cell differentiation in thymus GO:0033077 IEA
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    labyrinthine layer blood vessel development GO:0060716 IEA
    anterior/posterior axis specification, embryo GO:0008595 IDA
    positive regulation of interferon-gamma production GO:0032729 IMP
Subcellular Localization
    integral component of membrane GO:0016021 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
    cell surface GO:0009986 IDA
    bicellular tight junction GO:0005923 IEA
    plasma membrane GO:0005886 TAS
    early endosome membrane GO:0031901 TAS
    Golgi membrane GO:0000139 IEA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
 Experiment description of studies that identified FZD5 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for FZD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GOPC  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 WNT5A 7474
Invivo Homo sapiens
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