Gene description for WNT5A
Gene name wingless-type MMTV integration site family, member 5A
Gene symbol WNT5A
Other names/aliases hWNT5A
Species Homo sapiens
 Database cross references - WNT5A
ExoCarta ExoCarta_7474
Vesiclepedia VP_7474
Entrez Gene 7474
HGNC 12784
MIM 164975
UniProt P41221  
 WNT5A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for WNT5A
Molecular Function
    cytokine activity GO:0005125 IEA
    frizzled binding GO:0005109 IPI
    receptor tyrosine kinase-like orphan receptor binding GO:0005115 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IMP
    receptor agonist activity GO:0048018 IC
    frizzled-2 binding GO:0005110 IEA
    transcription regulatory region DNA binding GO:0044212 IDA
    protein domain specific binding GO:0019904 IEA
Biological Process
    positive regulation of protein catabolic process GO:0045732 IGI
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    urinary bladder development GO:0060157 IEA
    response to organic substance GO:0010033 IEP
    embryonic digit morphogenesis GO:0042733 IEA
    positive regulation of thymocyte apoptotic process GO:0070245 IEA
    keratinocyte differentiation GO:0030216 IEP
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IEA
    cellular response to lipopolysaccharide GO:0071222 IEP
    positive regulation of inflammatory response GO:0050729 IMP
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    primitive streak formation GO:0090009 IEA
    epithelial cell proliferation involved in mammary gland duct elongation GO:0060750 IEA
    regulation of branching involved in mammary gland duct morphogenesis GO:0060762 IEA
    embryonic skeletal system development GO:0048706 IMP
    type B pancreatic cell development GO:0003323 IEA
    cochlea morphogenesis GO:0090103 IEA
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IDA
    planar cell polarity pathway involved in pericardium morphogenesis GO:0061354 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEP
    male gonad development GO:0008584 IEP
    cellular protein localization GO:0034613 IDA
    cellular response to interferon-gamma GO:0071346 IEP
    positive regulation of cartilage development GO:0061036 IEA
    positive regulation of meiotic nuclear division GO:0045836 IEA
    cellular response to calcium ion GO:0071277 IEP
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    olfactory bulb interneuron development GO:0021891 ISS
    positive regulation of macrophage activation GO:0043032 IMP
    genitalia development GO:0048806 IMP
    anterior/posterior axis specification, embryo GO:0008595 IEA
    positive regulation of angiogenesis GO:0045766 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    lung development GO:0030324 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    ameboidal-type cell migration GO:0001667 IEA
    hypophysis morphogenesis GO:0048850 IEA
    dopaminergic neuron differentiation GO:0071542 IEA
    convergent extension involved in organogenesis GO:0060029 IEA
    positive regulation of protein kinase C activity GO:1900020 IMP
    establishment of planar polarity GO:0001736 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    negative regulation of fat cell differentiation GO:0045599 IMP
    negative regulation of mesenchymal cell proliferation GO:0072201 IDA
    vagina development GO:0060068 IEA
    cervix development GO:0060067 IEA
    hematopoietic stem cell proliferation GO:0071425 IDA
    canonical Wnt signaling pathway GO:0060070 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of cytokine secretion involved in immune response GO:0002741 IMP
    hindgut morphogenesis GO:0007442 IEA
    positive regulation of interleukin-8 secretion GO:2000484 IEA
    post-anal tail morphogenesis GO:0036342 IEA
    lens development in camera-type eye GO:0002088 ISS
    positive regulation of ossification GO:0045778 IMP
    mesenchymal-epithelial cell signaling GO:0060638 IEA
    somitogenesis GO:0001756 IEA
    neuron differentiation GO:0030182 ISS
    optic cup formation involved in camera-type eye development GO:0003408 ISS
    activation of JUN kinase activity GO:0007257 IMP
    cellular response to retinoic acid GO:0071300 ISS
    planar cell polarity pathway involved in cardiac muscle tissue morphogenesis GO:0061350 IEA
    positive regulation of interleukin-6 production GO:0032755 IMP
    negative regulation of prostatic bud formation GO:0060686 IEA
    negative regulation of synapse assembly GO:0051964 IEA
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IEA
    palate development GO:0060021 IMP
    cartilage development GO:0051216 IEA
    planar cell polarity pathway involved in neural tube closure GO:0090179 IEA
    positive regulation of endothelial cell migration GO:0010595 IMP
    positive regulation of fibroblast proliferation GO:0048146 IDA
    positive regulation of response to cytokine stimulus GO:0060760 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IMP
    uterus development GO:0060065 IEA
    midgut development GO:0007494 IEA
    epithelial to mesenchymal transition GO:0001837 IEP
    negative regulation of axon extension involved in axon guidance GO:0048843 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    Wnt signaling pathway GO:0016055 IDA
    heart looping GO:0001947 IEA
    Wnt signaling pathway, calcium modulating pathway GO:0007223 IMP
    positive regulation of neuron projection development GO:0010976 ISS
    negative regulation of BMP signaling pathway GO:0030514 IEA
    face development GO:0060324 IMP
    lateral sprouting involved in mammary gland duct morphogenesis GO:0060599 IEA
    mammary gland branching involved in thelarche GO:0060744 IEA
    positive regulation of chemokine biosynthetic process GO:0045080 IMP
    positive regulation of macrophage cytokine production GO:0060907 IMP
    positive regulation of interferon-gamma production GO:0032729 IEA
    activation of MAPK activity GO:0000187 IDA
    activation of protein kinase B activity GO:0032148 IDA
    negative chemotaxis GO:0050919 IEA
    positive regulation of T cell chemotaxis GO:0010820 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    cell fate commitment GO:0045165 IBA
    positive regulation of protein kinase C signaling GO:0090037 IMP
    planar cell polarity pathway involved in cardiac right atrium morphogenesis GO:0061349 IEA
    planar cell polarity pathway involved in outflow tract morphogenesis GO:0061347 IEA
    positive regulation of cGMP metabolic process GO:0030825 IDA
    planar cell polarity pathway involved in ventricular septum morphogenesis GO:0061348 IEA
    wound healing GO:0042060 IDA
    axon guidance GO:0007411 ISS
    positive regulation of interleukin-1 beta secretion GO:0050718 IMP
    non-canonical Wnt signaling pathway via JNK cascade GO:0038031 IEA
Subcellular Localization
    cell surface GO:0009986 IEA
    extracellular region GO:0005576 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    proteinaceous extracellular matrix GO:0005578 IEA
    extracellular space GO:0005615 IDA
    Golgi lumen GO:0005796 TAS
    extracellular exosome GO:0070062 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified WNT5A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WNT5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FZD1 8321
Affinity Capture-Western Homo sapiens
2 FZD5 7855
Invivo Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here