Gene description for ST3GAL4
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Gene symbol ST3GAL4
Other names/aliases CGS23
NANTA3
SAT3
SIAT4
SIAT4C
ST3GalIV
STZ
Species Homo sapiens
 Database cross references - ST3GAL4
ExoCarta ExoCarta_6484
Vesiclepedia VP_6484
Entrez Gene 6484
HGNC 10864
MIM 104240
UniProt Q11206  
 ST3GAL4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ST3GAL4
Molecular Function
    monosialoganglioside sialyltransferase activity GO:0047288 IEA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IEA
Biological Process
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    O-glycan processing GO:0016266 TAS
    small molecule metabolic process GO:0044281 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    cognition GO:0050890 IMP
    post-translational protein modification GO:0043687 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    carbohydrate metabolic process GO:0005975 TAS
    sialylation GO:0097503 TAS
    keratan sulfate metabolic process GO:0042339 TAS
    protein O-linked glycosylation GO:0006493 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    Golgi cisterna membrane GO:0032580 IEA
    Golgi membrane GO:0000139 TAS
    integral component of Golgi membrane GO:0030173 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified ST3GAL4 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ST3GAL4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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