Gene description for PARP16
Gene name poly (ADP-ribose) polymerase family, member 16
Gene symbol PARP16
Other names/aliases ARTD15
C15orf30
pART15
Species Homo sapiens
 Database cross references - PARP16
ExoCarta ExoCarta_54956
Vesiclepedia VP_54956
Entrez Gene 54956
HGNC 26040
UniProt Q8N5Y8  
 PARP16 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for PARP16
Molecular Function
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IBA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IMP
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 TAS
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    kinase binding GO:0019900 IBA
    kinase binding GO:0019900 IPI
    protein serine/threonine kinase activator activity GO:0043539 IBA
    protein serine/threonine kinase activator activity GO:0043539 IMP
    NAD+-protein-lysine ADP-ribosyltransferase activity GO:0140804 IDA
    NAD+-protein-aspartate ADP-ribosyltransferase activity GO:0140806 IDA
    NAD+-protein-glutamate ADP-ribosyltransferase activity GO:0140807 IDA
    NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity GO:0140822 IEA
    NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity GO:0140844 IEA
    NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity GO:0140867 IEA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IDA
Biological Process
    viral protein processing GO:0019082 TAS
    endoplasmic reticulum unfolded protein response GO:0030968 IBA
    endoplasmic reticulum unfolded protein response GO:0030968 IMP
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 TAS
    IRE1-mediated unfolded protein response GO:0036498 IMP
    protein auto-ADP-ribosylation GO:0070213 IDA
    protein auto-ADP-ribosylation GO:0070213 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEA
    negative regulation of cytoplasmic translation GO:2000766 IDA
Subcellular Localization
    nuclear envelope GO:0005635 IBA
    nuclear envelope GO:0005635 IDA
    endoplasmic reticulum GO:0005783 IMP
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    endoplasmic reticulum tubular network GO:0071782 IBA
    endoplasmic reticulum tubular network GO:0071782 IMP
 Experiment description of studies that identified PARP16 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for PARP16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF644  
Affinity Capture-MS Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 HACD3 51495
Affinity Capture-MS Homo sapiens
5 Cyr61  
Affinity Capture-MS Mus musculus
6 TMED10 10972
Affinity Capture-MS Homo sapiens
7 VWA8 23078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NEDD4 4734
Reconstituted Complex Homo sapiens
9 CHRNA4  
Affinity Capture-MS Homo sapiens
10 RPN2 6185
Proximity Label-MS Homo sapiens
11 MDN1 23195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PGRMC1 10857
Affinity Capture-MS Homo sapiens
13 UBE4A 9354
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 VAC14 55697
Affinity Capture-MS Homo sapiens
15 CRELD1 78987
Affinity Capture-MS Homo sapiens
16 EMD 2010
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PCIF1 63935
Affinity Capture-MS Homo sapiens
18 Setd5 72895
Affinity Capture-MS Mus musculus
19 NIPAL1  
Affinity Capture-MS Homo sapiens
20 SLC7A5 8140
Affinity Capture-MS Homo sapiens
21 NDUFA4 4697
Affinity Capture-MS Homo sapiens
22 RAI14 26064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KLC4 89953
Affinity Capture-MS Homo sapiens
24 DOT1L 84444
Affinity Capture-MS Homo sapiens
25 COG1 9382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SCN4A  
Affinity Capture-MS Homo sapiens
27 MRPL1  
Affinity Capture-MS Homo sapiens
28 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DDX20 11218
Affinity Capture-MS Homo sapiens
30 SEC62 7095
Proximity Label-MS Homo sapiens
31 LRP6 4040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LRP5 4041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RAB7A 7879
Proximity Label-MS Homo sapiens
34 GPC3 2719
Affinity Capture-MS Homo sapiens
35 B3GALNT2 148789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LRBA 987
Affinity Capture-MS Homo sapiens
37 GMCL1  
Two-hybrid Homo sapiens
38 COG5 10466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NEDD1 121441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SEC61B 10952
Proximity Label-MS Homo sapiens
41 MLKL 197259
Affinity Capture-MS Homo sapiens
42 ECSIT 51295
Affinity Capture-MS Homo sapiens
43 CCDC47 57003
Affinity Capture-MS Homo sapiens
44 HYPM  
Affinity Capture-MS Homo sapiens
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