Gene description for VAC14
Gene name Vac14 homolog (S. cerevisiae)
Gene symbol VAC14
Other names/aliases ArPIKfyve
TAX1BP2
TRX
Species Homo sapiens
 Database cross references - VAC14
ExoCarta ExoCarta_55697
Entrez Gene 55697
HGNC 25507
MIM 604632
UniProt Q08AM6  
 VAC14 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for VAC14
Molecular Function
    protein binding GO:0005515 IPI
    receptor activity GO:0004872 NAS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    signal transduction GO:0007165 NAS
    viral process GO:0016032 IEA
    regulation of lipid kinase activity GO:0043550 IEA
    small molecule metabolic process GO:0044281 TAS
    phospholipid metabolic process GO:0006644 TAS
Subcellular Localization
    early endosome membrane GO:0031901 TAS
    endosome membrane GO:0010008 IDA
    Golgi membrane GO:0000139 TAS
    PAS complex GO:0070772 IEA
    endoplasmic reticulum GO:0005783 IEA
    late endosome membrane GO:0031902 TAS
 Experiment description of studies that identified VAC14 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VAC14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
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