Gene description for VAC14
Gene name Vac14 homolog (S. cerevisiae)
Gene symbol VAC14
Other names/aliases ArPIKfyve
TAX1BP2
TRX
Species Homo sapiens
 Database cross references - VAC14
ExoCarta ExoCarta_55697
Vesiclepedia VP_55697
Entrez Gene 55697
HGNC 25507
MIM 604632
UniProt Q08AM6  
 VAC14 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for VAC14
Molecular Function
    protein binding GO:0005515 IPI
    signaling receptor activity GO:0038023 NAS
    identical protein binding GO:0042802 IPI
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 IBA
    signal transduction GO:0007165 NAS
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IDA
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 IDA
    early endosome membrane GO:0031901 TAS
    late endosome membrane GO:0031902 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    PAS complex GO:0070772 IBA
    presynaptic endosome GO:0098830 IEA
 Experiment description of studies that identified VAC14 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VAC14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MBLAC1 255374
Two-hybrid Homo sapiens
2 MVP 9961
Two-hybrid Homo sapiens
3 AMT  
Two-hybrid Homo sapiens
4 CABP2  
Affinity Capture-MS Homo sapiens
5 CLCN7 1186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CCT3 7203
Co-fractionation Homo sapiens
7 CCDC103  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 OIT3 170392
Two-hybrid Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
10 CRYBA1  
Two-hybrid Homo sapiens
11 HCST  
Affinity Capture-MS Homo sapiens
12 UFSP1  
Two-hybrid Homo sapiens
13 C19orf54  
Two-hybrid Homo sapiens
14 QKI 9444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ZNF34  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 TMEM206 55248
Affinity Capture-MS Homo sapiens
17 ANK1 286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LSM4 25804
Co-fractionation Homo sapiens
19 SIGLEC5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 LAMP2 3920
Proximity Label-MS Homo sapiens
21 KRT82 3888
Two-hybrid Homo sapiens
22 TRIM55  
Two-hybrid Homo sapiens
23 RHOXF2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 APP 351
Reconstituted Complex Homo sapiens
25 PRR13  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 APEX1 328
Affinity Capture-RNA Homo sapiens
27 TOP2A 7153
Affinity Capture-MS Homo sapiens
28 CASP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 PELI1 57162
Two-hybrid Homo sapiens
30 LPAL2  
Two-hybrid Homo sapiens
31 GABPB2  
Two-hybrid Homo sapiens
32 WDR83  
Two-hybrid Homo sapiens
33 COL8A1 1295
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 PCMTD2  
Two-hybrid Homo sapiens
35 TMBIM6 7009
Affinity Capture-MS Homo sapiens
36 C4orf33  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 SLC27A2 11001
Affinity Capture-MS Homo sapiens
38 RAB21 23011
Affinity Capture-MS Homo sapiens
39 ITFG3 83986
Affinity Capture-MS Homo sapiens
40 FIG4 9896
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 KRTAP19-5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 ARID5A  
Two-hybrid Homo sapiens
43 DHODH 1723
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PHF1  
Two-hybrid Homo sapiens
45 MAVS 57506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SGK494  
Two-hybrid Homo sapiens
47 PBLD 64081
Two-hybrid Homo sapiens
48 TEX37  
Two-hybrid Homo sapiens
49 PRR20C  
Two-hybrid Homo sapiens
50 GPR17 2840
Affinity Capture-MS Homo sapiens
51 C11orf54 28970
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 PRR20A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 MICA 100507436
Affinity Capture-MS Homo sapiens
54 KIAA1715 80856
Affinity Capture-MS Homo sapiens
55 BSG 682
Affinity Capture-MS Homo sapiens
56 MTCH2 23788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HS2ST1 9653
Affinity Capture-MS Homo sapiens
58 KRTAP15-1  
Two-hybrid Homo sapiens
59 ACSS1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 VHLL  
Two-hybrid Homo sapiens
61 PARK2  
Affinity Capture-MS Homo sapiens
62 GLIS2  
Two-hybrid Homo sapiens
63 LINC00518  
Two-hybrid Homo sapiens
64 PHKA1 5255
Co-fractionation Homo sapiens
65 PPIP5K2 23262
Co-fractionation Homo sapiens
66 PRADC1  
Two-hybrid Homo sapiens
67 TMEM43 79188
Affinity Capture-MS Homo sapiens
68 COMTD1 118881
Affinity Capture-MS Homo sapiens
69 KRTAP6-2  
Two-hybrid Homo sapiens
70 ALS2CR11  
Two-hybrid Homo sapiens
71 ABHD11 83451
Two-hybrid Homo sapiens
72 WDYHV1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 NTRK1 4914
Affinity Capture-MS Homo sapiens
74 PIKFYVE 200576
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
75 GTF3C2  
Two-hybrid Homo sapiens
76 PPP2CA 5515
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 RHBDL1  
Two-hybrid Homo sapiens
78 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 PIGS 94005
Affinity Capture-MS Homo sapiens
80 GNAI2 2771
Affinity Capture-MS Homo sapiens
81 ARL13B 200894
Affinity Capture-MS Homo sapiens
82 VENTX  
Two-hybrid Homo sapiens
83 ARSE 415
Affinity Capture-MS Homo sapiens
84 ZBTB49 166793
Two-hybrid Homo sapiens
85 TRIM63  
Two-hybrid Homo sapiens
86 SMUG1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 SPRYD7 57213
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 EIF4A2 1974
Affinity Capture-MS Homo sapiens
89 DDAH2 23564
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 NEURL4  
Affinity Capture-MS Homo sapiens
91 FNDC3B 64778
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
92 MEOX2  
Two-hybrid Homo sapiens
93 MYOZ1  
Two-hybrid Homo sapiens
94 TP53BP1 7158
Two-hybrid Homo sapiens
95 XPO1 7514
Affinity Capture-MS Homo sapiens
96 EIF6 3692
Two-hybrid Homo sapiens
97 FAM50B  
Two-hybrid Homo sapiens
98 NPTN 27020
Affinity Capture-MS Homo sapiens
99 CD274 29126
Affinity Capture-MS Homo sapiens
100 ERGIC3 51614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 VAMP3 9341
Affinity Capture-MS Homo sapiens
102 CUL3 8452
Affinity Capture-MS Homo sapiens
103 PRR20D  
Two-hybrid Homo sapiens
104 BHLHE40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
105 EGFR 1956
Affinity Capture-MS Homo sapiens
106 OMA1  
Affinity Capture-MS Homo sapiens
107 METTL21B  
Two-hybrid Homo sapiens
108 SNF8 11267
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
109 HDHD3 81932
Two-hybrid Homo sapiens
110 GINS3  
Two-hybrid Homo sapiens
111 PRR20B  
Two-hybrid Homo sapiens
112 TSPAN8 7103
Affinity Capture-MS Homo sapiens
113 RPA3 6119
Proximity Label-MS Homo sapiens
114 RAB9A 9367
Proximity Label-MS Homo sapiens
115 MPZL1 9019
Affinity Capture-MS Homo sapiens
116 PPP6C 5537
Two-hybrid Homo sapiens
117 DDRGK1 65992
Affinity Capture-MS Homo sapiens
118 KRTAP6-1  
Two-hybrid Homo sapiens
119 TCEANC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
120 CPSF3L 54973
Two-hybrid Homo sapiens
121 METTL15  
Two-hybrid Homo sapiens
122 PARP16 54956
Affinity Capture-MS Homo sapiens
123 DPH1  
Affinity Capture-MS Homo sapiens
124 KHDC1  
Two-hybrid Homo sapiens
125 PFAS 5198
Co-fractionation Homo sapiens
126 MTMR9 66036
Two-hybrid Homo sapiens
127 CCBL1 883
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
128 EIF2S1 1965
Two-hybrid Homo sapiens
129 MGAT5B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
130 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
131 SLMAP 7871
Affinity Capture-MS Homo sapiens
132 ATP2B4 493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 PPIP5K1 9677
Co-fractionation Homo sapiens
134 CD209 30835
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
135 VAC14 55697
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
136 KRTAP19-3  
Two-hybrid Homo sapiens
137 RIPPLY1  
Two-hybrid Homo sapiens
138 EHHADH 1962
Two-hybrid Homo sapiens
139 SLC26A6 65010
Affinity Capture-MS Homo sapiens
140 JMJD7  
Two-hybrid Homo sapiens
141 WRAP53  
Two-hybrid Homo sapiens
142 ASB13  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
143 NHLRC2 374354
Affinity Capture-MS Homo sapiens
144 RNASEH2C 84153
Two-hybrid Homo sapiens
145 CYTH2 9266
Affinity Capture-MS Homo sapiens
146 PHYKPL  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
147 NUDT19 390916
Affinity Capture-MS Homo sapiens
148 MFSD4  
Affinity Capture-MS Homo sapiens
149 C1orf106  
Affinity Capture-MS Homo sapiens
150 KRTAP19-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
151 OTOS  
Two-hybrid Homo sapiens
152 CD80 941
Affinity Capture-MS Homo sapiens
153 RBFOX2 23543
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
154 ERGIC2 51290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 PRR20E  
Two-hybrid Homo sapiens
156 ME1 4199
Affinity Capture-MS Homo sapiens
157 RAB7A 7879
Proximity Label-MS Homo sapiens
158 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 CRYBA2  
Two-hybrid Homo sapiens
160 TPST2 8459
Affinity Capture-MS Homo sapiens
161 PDE4DIP 9659
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
162 HNF1B  
Two-hybrid Homo sapiens
163 CDRT4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
164 PRAF2 11230
Affinity Capture-MS Homo sapiens
165 PTCH1  
Affinity Capture-MS Homo sapiens
166 NGB  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
167 ZNF720  
Two-hybrid Homo sapiens
168 MPPED2  
Two-hybrid Homo sapiens
169 MGST3 4259
Affinity Capture-MS Homo sapiens
170 CUL4A 8451
Affinity Capture-MS Homo sapiens
171 EP300 2033
Affinity Capture-MS Homo sapiens
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