Experiment description of studies that identified PBLD in sEVs

1 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for PBLD

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 BAG3 9531 Affinity Capture-Luminescence Homo sapiens 3 TRIP13 9319 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 4 ZRANB1 54764 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 PBLD 64081 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 6 EFHC2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 7 VAC14 55697 Two-hybrid Homo sapiens 8 MATN3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 ELMOD1   Affinity Capture-MS Homo sapiens 10 PHYH 5264 Co-fractionation Homo sapiens 11 STRAP 11171 Two-hybrid Homo sapiens 12 TRMT61A 115708 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 TCF4   Two-hybrid Homo sapiens 14 MIOX   Affinity Capture-MS Homo sapiens 15 NRBF2   Affinity Capture-MS Homo sapiens 16 BEND7 222389 Two-hybrid Homo sapiens 17 PLEKHS1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 AMOT 154796 Two-hybrid Homo sapiens 19 FCAR   Affinity Capture-MS Homo sapiens 20 LNX1   Two-hybrid Homo sapiens 21 CCDC184   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 22 CUL4A 8451 Affinity Capture-MS Homo sapiens View the network

Pathways in which PBLD is involved

No pathways found