Gene description for TMEM43
Gene name transmembrane protein 43
Gene symbol TMEM43
Other names/aliases ARVC5
ARVD5
EDMD7
LUMA
Species Homo sapiens
 Database cross references - TMEM43
ExoCarta ExoCarta_79188
Vesiclepedia VP_79188
Entrez Gene 79188
HGNC 28472
MIM 612048
UniProt Q9BTV4  
 TMEM43 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for TMEM43
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    lipid metabolic process GO:0006629 IBA
    innate immune response GO:0045087 IEA
    nuclear membrane organization GO:0071763 IBA
    nuclear membrane organization GO:0071763 IDA
Subcellular Localization
    nuclear inner membrane GO:0005637 IBA
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified TMEM43 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMEM43
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 TM4SF5  
Affinity Capture-MS Homo sapiens
4 Tmed2 56334
Affinity Capture-MS Mus musculus
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 VAPA 9218
Affinity Capture-MS Homo sapiens
7 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
9 BZW2 28969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ACKR2  
Affinity Capture-MS Homo sapiens
11 CFC1  
Affinity Capture-MS Homo sapiens
12 SLC2A1 6513
Affinity Capture-MS Homo sapiens
13 RPL14 9045
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
14 EIF4A1 1973
Co-fractionation Homo sapiens
15 Rab5c 19345
Affinity Capture-MS Mus musculus
16 Ktn1  
Affinity Capture-MS Mus musculus
17 RTN4 57142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 Tmem109  
Affinity Capture-MS Mus musculus
19 SLC12A4 6560
Co-fractionation Homo sapiens
20 RB1CC1 9821
Affinity Capture-MS Homo sapiens
21 KIAA1429 25962
Affinity Capture-MS Homo sapiens
22 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
23 MRPS5 64969
Co-fractionation Homo sapiens
24 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 C1qbp 12261
Affinity Capture-MS Mus musculus
26 DDOST 1650
Affinity Capture-MS Homo sapiens
27 RTN1 6252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RPN2 6185
Affinity Capture-MS Homo sapiens
29 TOR3A 64222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 TMEM106A  
Affinity Capture-MS Homo sapiens
32 FAF2 23197
Affinity Capture-MS Homo sapiens
33 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
34 TMEM59 9528
Affinity Capture-MS Homo sapiens
35 FAM20C 56975
Affinity Capture-MS Homo sapiens
36 KIF14 9928
Affinity Capture-MS Homo sapiens
37 Tnpo1 238799
Affinity Capture-MS Mus musculus
38 E2F4  
Affinity Capture-MS Homo sapiens
39 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 FGA 2243
Two-hybrid Homo sapiens
41 PPP6R2 9701
Affinity Capture-MS Homo sapiens
42 APOA5 116519
Two-hybrid Homo sapiens
43 APLP2 334
Affinity Capture-MS Homo sapiens
44 UBAC2 337867
Affinity Capture-MS Homo sapiens
45 SCGB1A1  
Two-hybrid Homo sapiens
46 SDF2L1 23753
Affinity Capture-MS Homo sapiens
47 VAPB 9217
Affinity Capture-Western Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 CAV2 858
Affinity Capture-MS Homo sapiens
50 DNAJB9 4189
Affinity Capture-MS Homo sapiens
51 ZMPSTE24 10269
Co-fractionation Homo sapiens
52 RPN1 6184
Affinity Capture-MS Homo sapiens
53 ARL13B 200894
Two-hybrid Homo sapiens
54 CD63 967
Affinity Capture-MS Homo sapiens
55 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ATP6V1E1 529
Co-fractionation Homo sapiens
57 BZW1 9689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 REEP5 7905
Affinity Capture-MS Homo sapiens
59 TM4SF20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 FANCD2  
Affinity Capture-MS Homo sapiens
61 ENDOD1 23052
Affinity Capture-MS Homo sapiens
62 CANX 821
Affinity Capture-MS Homo sapiens
63 SYNDIG1  
Two-hybrid Homo sapiens
64 LRRC59 55379
Proximity Label-MS Homo sapiens
65 CLTA 1211
Affinity Capture-MS Homo sapiens
66 GGH 8836
Affinity Capture-MS Homo sapiens
67 SRSF1 6426
Co-fractionation Homo sapiens
68 Bmpr1a  
Affinity Capture-MS Mus musculus
69 GOLT1B 51026
Affinity Capture-MS Homo sapiens
70 DAD1 1603
Affinity Capture-MS Homo sapiens
71 RMND1 55005
Affinity Capture-MS Homo sapiens
72 FKBP8 23770
Two-hybrid Homo sapiens
73 GPRASP2  
Affinity Capture-MS Homo sapiens
74 FDFT1 2222
Two-hybrid Homo sapiens
75 FAM209A 200232
Two-hybrid Homo sapiens
76 SAAL1 113174
Affinity Capture-MS Homo sapiens
77 TSPAN8 7103
Affinity Capture-MS Homo sapiens
78 RPA3 6119
Proximity Label-MS Homo sapiens
79 PCYT1B 9468
Two-hybrid Homo sapiens
80 CMTM5  
Affinity Capture-MS Homo sapiens
81 NAALADL1  
Affinity Capture-MS Homo sapiens
82 PLIN3 10226
Affinity Capture-MS Homo sapiens
83 PTPRN  
Affinity Capture-MS Homo sapiens
84 DDRGK1 65992
Affinity Capture-MS Homo sapiens
85 RNF26  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
86 ZCCHC12  
Two-hybrid Homo sapiens
87 REEP6  
Affinity Capture-MS Homo sapiens
88 OST4  
Affinity Capture-MS Homo sapiens
89 CLPP 8192
Proximity Label-MS Homo sapiens
90 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
91 TSPAN5 10098
Affinity Capture-MS Homo sapiens
92 TMEM109 79073
Affinity Capture-MS Homo sapiens
93 TRIM13  
Affinity Capture-MS Homo sapiens
94 USP25  
Two-hybrid Homo sapiens
95 MCAT 27349
Co-fractionation Homo sapiens
96 FANCC 2176
Affinity Capture-MS Homo sapiens
97 EXOC8 149371
Affinity Capture-MS Homo sapiens
98 SPAG6  
Affinity Capture-MS Homo sapiens
99 PDIA4 9601
Proximity Label-MS Homo sapiens
100 TTC39C 125488
Affinity Capture-MS Homo sapiens
101 VAC14 55697
Affinity Capture-MS Homo sapiens
102 DDX58 23586
Affinity Capture-RNA Homo sapiens
103 MMGT1 93380
Affinity Capture-MS Homo sapiens
104 SFTPC  
Affinity Capture-MS Homo sapiens
105 UFL1 23376
Affinity Capture-MS Homo sapiens
106 GRAMD3  
Two-hybrid Homo sapiens
107 TBRG4 9238
Affinity Capture-MS Homo sapiens
108 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
110 RAB11A 8766
Affinity Capture-MS Homo sapiens
111 CISD2 493856
Two-hybrid Homo sapiens
112 SLC8A3  
Affinity Capture-MS Homo sapiens
113 COG7 91949
Affinity Capture-MS Homo sapiens
114 BRI3BP 140707
Affinity Capture-MS Homo sapiens
115 DDX28  
Co-fractionation Homo sapiens
116 APOA1 335
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM43 is involved
No pathways found





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