Gene description for PCYT1B
Gene name phosphate cytidylyltransferase 1, choline, beta
Gene symbol PCYT1B
Other names/aliases CCTB
CTB
Species Homo sapiens
 Database cross references - PCYT1B
ExoCarta ExoCarta_9468
Entrez Gene 9468
HGNC 8755
MIM 300948
UniProt Q9Y5K3  
 PCYT1B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PCYT1B
Molecular Function
    choline-phosphate cytidylyltransferase activity GO:0004105 IEA
Biological Process
    phospholipid metabolic process GO:0006644 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    spermatogenesis GO:0007283 IEA
    phosphatidylcholine biosynthetic process GO:0006656 TAS
    small molecule metabolic process GO:0044281 TAS
    CDP-choline pathway GO:0006657 TAS
    ovarian follicle development GO:0001541 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified PCYT1B in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCYT1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTA1 58
Invitro Homo sapiens
2 ACTA2 59
Invitro Homo sapiens
3 ACTB 60
Co-crystal Structure Homo sapiens
View the network image/svg+xml
 Pathways in which PCYT1B is involved
PathwayEvidenceSource
Synthesis of PC TAS Reactome





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