Gene description for CLCN7
Gene name chloride channel, voltage-sensitive 7
Gene symbol CLCN7
Other names/aliases CLC-7
CLC7
OPTA2
OPTB4
PPP1R63
Species Homo sapiens
 Database cross references - CLCN7
ExoCarta ExoCarta_1186
Vesiclepedia VP_1186
Entrez Gene 1186
HGNC 2025
MIM 602727
UniProt P51798  
 CLCN7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CLCN7
Molecular Function
    voltage-gated chloride channel activity GO:0005247 IEA
    chloride channel activity GO:0005254 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    chloride transmembrane transporter activity GO:0015108 IBA
    antiporter activity GO:0015297 IEA
Biological Process
    transepithelial chloride transport GO:0030321 IDA
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    membrane GO:0016020 HDA
    chloride channel complex GO:0034707 IPI
    intracellular membrane-bounded organelle GO:0043231 IBA
 Experiment description of studies that identified CLCN7 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 255
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 256
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 257
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CLCN7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 Tmed2 56334
Affinity Capture-MS Mus musculus
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 STK25 10494
Affinity Capture-MS Homo sapiens
5 MBLAC2 153364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RXFP1  
Affinity Capture-MS Homo sapiens
7 OR10H2  
Affinity Capture-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 LAMP3  
Proximity Label-MS Homo sapiens
10 CD53 963
Two-hybrid Homo sapiens
11 Atp2a2 11938
Affinity Capture-MS Mus musculus
12 HSD17B11 51170
Proximity Label-MS Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 SAMD1  
Affinity Capture-MS Homo sapiens
15 Ktn1  
Affinity Capture-MS Mus musculus
16 STX4 6810
Proximity Label-MS Homo sapiens
17 CTXN1  
Affinity Capture-MS Homo sapiens
18 TMEM179B 374395
Two-hybrid Homo sapiens
19 Kcnk1  
Affinity Capture-MS Mus musculus
20 RAB3B 5865
Proximity Label-MS Homo sapiens
21 OPRM1 4988
Two-hybrid Homo sapiens
22 STX7 8417
Proximity Label-MS Homo sapiens
23 SSR1 6745
Proximity Label-MS Homo sapiens
24 C1qbp 12261
Affinity Capture-MS Mus musculus
25 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 C3orf52  
Affinity Capture-MS Homo sapiens
27 TTYH1  
Affinity Capture-MS Homo sapiens
28 STX6 10228
Proximity Label-MS Homo sapiens
29 SLC15A4 121260
Affinity Capture-MS Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 Vps28 66914
Affinity Capture-MS Mus musculus
32 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PQLC1  
Two-hybrid Homo sapiens
34 LAMTOR1 55004
Proximity Label-MS Homo sapiens
35 TMEM63B 55362
Affinity Capture-MS Homo sapiens
36 LMAN1 3998
Proximity Label-MS Homo sapiens
37 NUP155 9631
Proximity Label-MS Homo sapiens
38 LMNB1 4001
Proximity Label-MS Homo sapiens
39 EMD 2010
Proximity Label-MS Homo sapiens
40 TMEM47 83604
Affinity Capture-MS Homo sapiens
41 MCAM 4162
Proximity Label-MS Homo sapiens
42 RAB4A 5867
Proximity Label-MS Homo sapiens
43 ACP2 53
Affinity Capture-MS Homo sapiens
44 DNAJC5 80331
Proximity Label-MS Homo sapiens
45 PCDHGC4  
Affinity Capture-MS Homo sapiens
46 LAMP1 3916
Proximity Label-MS Homo sapiens
47 CREB3  
Two-hybrid Homo sapiens
48 RPN1 6184
Proximity Label-MS Homo sapiens
49 RNF13 11342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TMEM56  
Two-hybrid Homo sapiens
51 LHFPL5  
Two-hybrid Homo sapiens
52 ELOVL5 60481
Proximity Label-MS Homo sapiens
53 CTDSPL 10217
Proximity Label-MS Homo sapiens
54 SEC61B 10952
Proximity Label-MS Homo sapiens
55 SLC33A1 9197
Affinity Capture-MS Homo sapiens
56 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
57 NKAIN1  
Affinity Capture-MS Homo sapiens
58 Rab5c 19345
Affinity Capture-MS Mus musculus
59 CYB5B 80777
Affinity Capture-MS Homo sapiens
60 RPN2 6185
Proximity Label-MS Homo sapiens
61 Cd2ap 12488
Affinity Capture-MS Mus musculus
62 Nup98  
Affinity Capture-MS Mus musculus
63 RAB7A 7879
Proximity Label-MS Homo sapiens
64 RAB5A 5868
Proximity Label-MS Homo sapiens
65 PANX1 24145
Proximity Label-MS Homo sapiens
66 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 Bmpr1a  
Affinity Capture-MS Mus musculus
68 GOLT1B 51026
Affinity Capture-MS Homo sapiens
69 BCAP31 10134
Proximity Label-MS Homo sapiens
70 HSD3B7 80270
Proximity Label-MS Homo sapiens
71 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
72 LGALS9B 284194
Affinity Capture-MS Homo sapiens
73 ARF6 382
Proximity Label-MS Homo sapiens
74 KCNN3  
Two-hybrid Homo sapiens
75 GDAP1 54332
Two-hybrid Homo sapiens
76 MYO6 4646
Proximity Label-MS Homo sapiens
77 WRB 7485
Two-hybrid Homo sapiens
78 ATP2A1 487
Proximity Label-MS Homo sapiens
79 RAB9A 9367
Proximity Label-MS Homo sapiens
80 FAM46A  
Affinity Capture-MS Homo sapiens
81 LYVE1  
Two-hybrid Homo sapiens
82 RHOB 388
Proximity Label-MS Homo sapiens
83 XRCC3  
Affinity Capture-MS Homo sapiens
84 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 TP53 7157
Affinity Capture-MS Homo sapiens
87 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
88 VAC14 55697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 LRRC55  
Affinity Capture-MS Homo sapiens
90 OSTM1 28962
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
91 SLC38A7 55238
Two-hybrid Homo sapiens
92 GORASP1 64689
Proximity Label-MS Homo sapiens
93 RAB2A 5862
Proximity Label-MS Homo sapiens
94 AQP7  
Two-hybrid Homo sapiens
95 SEC62 7095
Proximity Label-MS Homo sapiens
96 B3GAT1  
Proximity Label-MS Homo sapiens
97 BLVRA 644
Affinity Capture-MS Homo sapiens
98 RAB11A 8766
Proximity Label-MS Homo sapiens
99 Flot1 14251
Affinity Capture-MS Mus musculus
100 KRAS 3845
Proximity Label-MS Homo sapiens
101 RAB5C 5878
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
102 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 BIRC7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 CAV1 857
Proximity Label-MS Homo sapiens
105 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLCN7 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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