Gene description for CLCN7
Gene name chloride channel, voltage-sensitive 7
Gene symbol CLCN7
Other names/aliases CLC-7
CLC7
OPTA2
OPTB4
PPP1R63
Species Homo sapiens
 Database cross references - CLCN7
ExoCarta ExoCarta_1186
Entrez Gene 1186
HGNC 2025
MIM 602727
UniProt P51798  
 CLCN7 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for CLCN7
Molecular Function
    ATP binding GO:0005524 IEA
    chloride channel activity GO:0005254 TAS
    antiporter activity GO:0015297 IEA
    voltage-gated chloride channel activity GO:0005247 IEA
Biological Process
    transmembrane transport GO:0055085 TAS
    ion transmembrane transport GO:0034220 TAS
    regulation of anion transmembrane transport GO:1903959 IEA
    chloride transmembrane transport GO:1902476 TAS
    transport GO:0006810 TAS
    response to pH GO:0009268 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 TAS
    cytoplasmic vesicle GO:0031410 IEA
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified CLCN7 in exosomes
1
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CLCN7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CLCN7 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





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