Gene description for Vps28
Gene name vacuolar protein sorting 28 (yeast)
Gene symbol Vps28
Other names/aliases 1110014J03Rik
CIIA
D730005C08Rik
Species Mus musculus
 Database cross references - Vps28
ExoCarta ExoCarta_66914
Vesiclepedia VP_66914
Entrez Gene 66914
UniProt Q9D1C8  
 Vps28 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps28
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
    protein-containing complex binding GO:0044877 IBA
Biological Process
    protein transport GO:0015031 IEA
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    endosome transport via multivesicular body sorting pathway GO:0032509 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISS
    protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 IEA
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 ISO
Subcellular Localization
    ESCRT I complex GO:0000813 IBA
    ESCRT I complex GO:0000813 ISO
    ESCRT I complex GO:0000813 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endosome membrane GO:0010008 ISO
    late endosome membrane GO:0031902 IEA
 Experiment description of studies that identified Vps28 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
3 Ubc  
Reconstituted Complex Mus musculus
4 MUC13 56667
Affinity Capture-MS Homo sapiens
5 CLCN7 1186
Affinity Capture-MS Homo sapiens
6 UBAP1 51271
Affinity Capture-MS Homo sapiens
7 C2orf69  
Affinity Capture-MS Homo sapiens
8 CUTA 51596
Affinity Capture-MS Homo sapiens
9 SEC23IP 11196
Affinity Capture-MS Homo sapiens
10 MYL6 4637
Affinity Capture-MS Homo sapiens
11 DHX36 170506
Affinity Capture-MS Homo sapiens
12 HBE1 3046
Affinity Capture-MS Homo sapiens
13 Fabp3  
Co-fractionation Mus musculus
14 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
15 Vps37c 107305
Co-fractionation Mus musculus
16 TSG101 7251
Affinity Capture-MS Homo sapiens
17 TAF10 6881
Affinity Capture-MS Homo sapiens
18 SLC35F6 54978
Affinity Capture-MS Homo sapiens
19 TAF1  
Affinity Capture-MS Homo sapiens
20 TBP  
Affinity Capture-MS Homo sapiens
21 Tmem173  
Proximity Label-MS Mus musculus
22 BAZ2A  
Affinity Capture-MS Homo sapiens
23 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
24 UMAD1  
Affinity Capture-MS Homo sapiens
25 VPS37A 137492
Affinity Capture-MS Homo sapiens
26 RBM26 64062
Affinity Capture-MS Homo sapiens
27 ATP6V1A 523
Affinity Capture-MS Homo sapiens
28 OBSCN 84033
Affinity Capture-MS Homo sapiens
29 MRPL3  
Affinity Capture-MS Homo sapiens
30 EPB41L4B 54566
Affinity Capture-MS Homo sapiens
31 HSPG2 3339
Affinity Capture-MS Homo sapiens
32 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
33 VPS37C 55048
Affinity Capture-MS Homo sapiens
34 CHMP2B 25978
Affinity Capture-MS Homo sapiens
35 FLOT2 2319
Affinity Capture-MS Homo sapiens
36 Tsg101 22088
Co-fractionation Mus musculus
37 FLOT1 10211
Affinity Capture-MS Homo sapiens
38 KIAA0556  
Affinity Capture-MS Homo sapiens
39 VPS37B 79720
Affinity Capture-MS Homo sapiens
40 MVB12A 93343
Affinity Capture-MS Homo sapiens
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