Gene description for Vps28
Gene name vacuolar protein sorting 28 (yeast)
Gene symbol Vps28
Other names/aliases 1110014J03Rik
CIIA
D730005C08Rik
Species Mus musculus
 Database cross references - Vps28
ExoCarta ExoCarta_66914
Entrez Gene 66914
UniProt Q9D1C8  
 Vps28 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
 Gene ontology annotations for Vps28
Molecular Function
    protein complex binding GO:0032403 IBA
    ubiquitin binding GO:0043130 ISO
    protein binding GO:0005515 IPI
Biological Process
    protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
Subcellular Localization
    ESCRT I complex GO:0000813 ISO
    extracellular exosome GO:0070062 ISO
    endosome membrane GO:0010008 ISO
    early endosome GO:0005769 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    endosome GO:0005768 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Vps28 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
 Protein-protein interactions for Vps28
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vps28 is involved
No pathways found





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