Gene description for Tsg101
Gene name tumor susceptibility gene 101
Gene symbol Tsg101
Other names/aliases AI255943
CC2
Species Mus musculus
 Database cross references - Tsg101
ExoCarta ExoCarta_22088
Entrez Gene 22088
UniProt Q61187  
 Tsg101 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Hepatocytes 19367702    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
 Gene ontology annotations for Tsg101
Molecular Function
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 ISO
    virion binding GO:0046790 ISO
    calcium-dependent protein binding GO:0048306 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 ISO
    protein binding GO:0005515 IPI
    transcription corepressor activity GO:0003714 IDA
    ubiquitin binding GO:0043130 ISO
Biological Process
    cell cycle arrest GO:0007050 IDA
    positive regulation of viral release from host cell GO:1902188 ISO
    protein transport GO:0015031 IEA
    transport GO:0006810 IEA
    regulation of extracellular exosome assembly GO:1903551 ISO
    cell differentiation GO:0030154 IDA
    positive regulation of viral process GO:0048524 ISO
    viral process GO:0016032 IEA
    viral budding GO:0046755 ISO
    endosome to lysosome transport GO:0008333 ISO
    regulation of cell growth GO:0001558 IDA
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 IMP
    keratinocyte differentiation GO:0030216 IDA
    regulation of nucleic acid-templated transcription GO:1903506 ISO
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    cell division GO:0051301 IEA
    regulation of viral budding via host ESCRT complex GO:1903772 ISO
    regulation of growth GO:0040008 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    negative regulation of cell proliferation GO:0008285 IDA
    cell cycle GO:0007049 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    regulation of MAP kinase activity GO:0043405 ISO
    protein monoubiquitination GO:0006513 ISO
    cellular protein modification process GO:0006464 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    late endosome GO:0005770 ISO
    membrane GO:0016020 IEA
    nucleolus GO:0005730 ISO
    early endosome GO:0005769 ISO
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 ISO
    ESCRT I complex GO:0000813 ISO
    endosome membrane GO:0010008 ISO
 Experiment description of studies that identified Tsg101 in exosomes
1
Experiment ID 10
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 36
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Mus musculus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name MLP-29
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 262
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90
EV Cytosolic markers
✔
CD63|CD9|
EV Membrane markers
✘ EV Negative markers
✔
Y
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
 Protein-protein interactions for Tsg101
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gcl  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Tsg101 is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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