Gene description for Gja1
Gene name gap junction protein, alpha 1
Gene symbol Gja1
Other names/aliases AU042049
AW546267
Cnx43
Cx43
Cx43alpha1
Gja-1
Npm1
connexin43
Species Mus musculus
 Database cross references - Gja1
ExoCarta ExoCarta_14609
Vesiclepedia VP_14609
Entrez Gene 14609
UniProt P23242  
 Gja1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gja1
Molecular Function
    protein domain specific binding GO:0019904 ISO
    transmembrane transporter activity GO:0022857 IEA
    ion transmembrane transporter activity GO:0015075 ISO
    scaffold protein binding GO:0097110 IPI
    gap junction channel activity GO:0005243 ISO
    PDZ domain binding GO:0030165 ISO
    SH3 domain binding GO:0017124 ISO
    receptor binding GO:0005102 IPI
    gap junction channel activity involved in cell communication by electrical coupling GO:1903763 ISO
    signal transducer activity GO:0004871 ISO
    connexin binding GO:0071253 ISO
    beta-tubulin binding GO:0048487 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of glomerular filtration GO:0003104 ISO
    regulation of bicellular tight junction assembly GO:2000810 ISO
    cardiac conduction GO:0061337 IMP
    chronic inflammatory response GO:0002544 ISO
    regulation of ventricular cardiac muscle cell membrane depolarization GO:0060373 IMP
    adult heart development GO:0007512 IMP
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    heart looping GO:0001947 IMP
    response to pH GO:0009268 ISO
    negative regulation of DNA biosynthetic process GO:2000279 ISO
    lens development in camera-type eye GO:0002088 IMP
    ATP transport GO:0015867 ISO
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    osteoblast differentiation GO:0001649 IMP
    epithelial cell maturation GO:0002070 IMP
    cell-cell signaling GO:0007267 ISO
    atrial ventricular junction remodeling GO:0003294 IGI
    negative regulation of cardiac muscle cell proliferation GO:0060044 ISO
    epicardial cell to mesenchymal cell transition GO:0003347 TAS
    regulation of heart contraction GO:0008016 IMP
    ion transmembrane transport GO:0034220 ISO
    positive regulation of insulin secretion GO:0032024 ISO
    regulation of blood vessel remodeling GO:0060312 TAS
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    skeletal muscle tissue regeneration GO:0043403 IDA
    negative regulation of cell proliferation GO:0008285 ISO
    positive regulation of cell communication by chemical coupling GO:0010652 ISO
    neuron migration GO:0001764 IMP
    embryonic heart tube development GO:0035050 IMP
    regulation of atrial cardiac muscle cell membrane depolarization GO:0060371 IMP
    signal transduction GO:0007165 ISO
    regulation of heart rate GO:0002027 ISO
    transmembrane transport GO:0055085 ISO
    regulation of bone remodeling GO:0046850 IMP
    protein oligomerization GO:0051259 ISO
    embryonic digit morphogenesis GO:0042733 IMP
    heart development GO:0007507 IMP
    cell communication by electrical coupling GO:0010644 ISO
    positive regulation of striated muscle tissue development GO:0045844 IMP
    positive regulation of osteoblast differentiation GO:0045669 IMP
    positive regulation of gene expression GO:0010628 IMP
    apoptotic process GO:0006915 ISO
    cell communication GO:0007154 ISO
    positive regulation of vasodilation GO:0045909 ISO
    positive regulation of behavioral fear response GO:2000987 ISO
    blood vessel morphogenesis GO:0048514 IMP
    neuron projection morphogenesis GO:0048812 ISO
    milk ejection GO:0060156 IMP
    vascular transport GO:0010232 ISO
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    negative regulation of wound healing GO:0061045 ISO
    positive regulation of vasoconstriction GO:0045907 ISO
    in utero embryonic development GO:0001701 IMP
    positive regulation of protein catabolic process GO:0045732 ISO
    establishment of epithelial cell polarity GO:0090162 TAS
    regulation of calcium ion transport GO:0051924 ISO
    cell-cell junction organization GO:0045216 IMP
    cell communication by chemical coupling GO:0010643 IDA
    regulation of bone mineralization GO:0030500 IMP
    negative regulation of gene expression GO:0010629 IMP
Subcellular Localization
    cell-cell junction GO:0005911 IDA
    late endosome GO:0005770 ISO
    cytosol GO:0005829 ISO
    lateral plasma membrane GO:0016328 IDA
    integral component of membrane GO:0016021 IEA
    membrane raft GO:0045121 ISO
    connexon complex GO:0005922 ISO
    fascia adherens GO:0005916 ISO
    Golgi apparatus GO:0005794 ISO
    contractile fiber GO:0043292 IDA
    extracellular exosome GO:0070062 ISO
    multivesicular body GO:0005771 ISO
    plasma membrane GO:0005886 ISO
    intercalated disc GO:0014704 ISO
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IDA
    focal adhesion GO:0005925 ISO
    cell junction GO:0030054 IDA
    intermediate filament GO:0005882 IDA
    cytoplasm GO:0005737 ISO
    mitochondrial outer membrane GO:0005741 ISO
    gap junction GO:0005921 ISO
    endosome GO:0005768 ISO
    lysosome GO:0005764 ISO
    early endosome GO:0005769 ISO
 Experiment description of studies that identified Gja1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gja1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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