Gene description for Eps15
Gene name epidermal growth factor receptor pathway substrate 15
Gene symbol Eps15
Other names/aliases 2410112D09Rik
Species Mus musculus
 Database cross references - Eps15
ExoCarta ExoCarta_13858
Vesiclepedia VP_13858
Entrez Gene 13858
UniProt P42567  
 Eps15 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eps15
Molecular Function
    metal ion binding GO:0046872 IEA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    polyubiquitin binding GO:0031593 ISO
    calcium ion binding GO:0005509 IEA
    SH3 domain binding GO:0017124 IEA
Biological Process
    viral entry into host cell GO:0046718 ISO
    protein transport GO:0015031 IEA
    receptor-mediated endocytosis of virus by host cell GO:0019065 ISO
    clathrin coat assembly GO:0048268 ISO
    positive regulation of receptor recycling GO:0001921 ISO
    endocytic recycling GO:0032456 ISO
    transport GO:0006810 IEA
    endocytosis GO:0006897 IEA
    regulation of cell proliferation GO:0042127 ISO
    Golgi to endosome transport GO:0006895 ISO
Subcellular Localization
    clathrin coat of coated pit GO:0030132 IDA
    aggresome GO:0016235 ISO
    plasma membrane GO:0005886 ISO
    coated pit GO:0005905 IDA
    early endosome GO:0005769 ISO
    apical plasma membrane GO:0016324 ISO
    ciliary membrane GO:0060170 IDA
    clathrin-coated vesicle GO:0030136 IDA
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    endosome GO:0005768 IEA
    AP-2 adaptor complex GO:0030122 IDA
    basal plasma membrane GO:0009925 ISO
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Eps15 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eps15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNM1 1759
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Eps15 is involved
PathwayEvidenceSource
EGFR downregulation IEA Reactome





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