Gene description for Ap2m1
Gene name adaptor-related protein complex 2, mu 1 subunit
Gene symbol Ap2m1
Other names/aliases -
Species Mus musculus
 Database cross references - Ap2m1
ExoCarta ExoCarta_11773
Vesiclepedia VP_11773
Entrez Gene 11773
UniProt P84091  
 Ap2m1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap2m1
Molecular Function
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    lipid binding GO:0008289 IEA
    signal sequence binding GO:0005048 ISO
Biological Process
    intracellular protein transport GO:0006886 TAS
    vesicle-mediated transport GO:0016192 TAS
    transport GO:0006810 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
    protein transport GO:0015031 IEA
    endocytosis GO:0006897 IEA
Subcellular Localization
    plasma membrane GO:0005886 IEA
    terminal bouton GO:0043195 ISO
    coated pit GO:0005905 IEA
    mitochondrion GO:0005739 IDA
    secretory granule GO:0030141 TAS
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    clathrin adaptor complex GO:0030131 IEA
 Experiment description of studies that identified Ap2m1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2m1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ctla4  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
View the network image/svg+xml



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