Gene description for Ap2m1
Gene name adaptor-related protein complex 2, mu 1 subunit
Gene symbol Ap2m1
Other names/aliases -
Species Mus musculus
 Database cross references - Ap2m1
ExoCarta ExoCarta_11773
Vesiclepedia VP_11773
Entrez Gene 11773
UniProt P84091  
 Ap2m1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap2m1
Molecular Function
    signal sequence binding GO:0005048 IEA
    signal sequence binding GO:0005048 ISO
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    clathrin adaptor activity GO:0035615 IBA
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    low-density lipoprotein particle receptor binding GO:0050750 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    disordered domain specific binding GO:0097718 IDA
Biological Process
    positive regulation of receptor internalization GO:0002092 IEA
    positive regulation of receptor internalization GO:0002092 ISO
    intracellular protein transport GO:0006886 TAS
    vesicle budding from membrane GO:0006900 IEA
    vesicle budding from membrane GO:0006900 ISO
    vesicle-mediated transport GO:0016192 NAS
    vesicle-mediated transport GO:0016192 TAS
    receptor internalization GO:0031623 ISO
    synaptic vesicle endocytosis GO:0048488 IEA
    synaptic vesicle endocytosis GO:0048488 ISO
    protein-containing complex assembly GO:0065003 IEA
    protein-containing complex assembly GO:0065003 ISO
    clathrin-dependent endocytosis GO:0072583 IBA
    clathrin-dependent endocytosis GO:0072583 IDA
    clathrin-dependent endocytosis GO:0072583 ISO
    clathrin-dependent endocytosis GO:0072583 NAS
    regulation of vesicle size GO:0097494 IEA
    regulation of vesicle size GO:0097494 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 IDA
    postsynaptic neurotransmitter receptor internalization GO:0098884 IMP
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
    positive regulation of synaptic vesicle endocytosis GO:1900244 IEA
    positive regulation of synaptic vesicle endocytosis GO:1900244 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 ISO
    synaptic vesicle GO:0008021 IEA
    cytoplasmic side of plasma membrane GO:0009898 NAS
    AP-2 adaptor complex GO:0030122 IBA
    AP-2 adaptor complex GO:0030122 IDA
    AP-2 adaptor complex GO:0030122 ISO
    AP-2 adaptor complex GO:0030122 NAS
    secretory granule GO:0030141 TAS
    cytoplasmic vesicle GO:0031410 IBA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    postsynapse GO:0098794 IEA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 IEA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ap2m1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2m1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snap91  
Co-fractionation Mus musculus
2 STK11 6794
Two-hybrid Homo sapiens
3 Zbtb7b  
Affinity Capture-MS Mus musculus
4 Eps15 13858
Co-fractionation Mus musculus
5 Ubc  
Reconstituted Complex Mus musculus
6 Tmem173  
Proximity Label-MS Mus musculus
7 SNCA 6622
Affinity Capture-MS Homo sapiens
8 Atxn1  
Affinity Capture-MS Mus musculus
9 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
10 Eed  
Affinity Capture-MS Mus musculus
11 Atg5  
Affinity Capture-MS Mus musculus
12 Ap2m1 11773
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Mapt  
Affinity Capture-MS Mus musculus
14 Ctla4  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
15 Atg16l1  
Affinity Capture-MS Mus musculus
16 Pou5f1  
Affinity Capture-MS Mus musculus
17 Mea1  
Co-fractionation Mus musculus
18 Sstr3  
Proximity Label-MS Mus musculus
19 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap2m1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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