Gene description for Itsn1
Gene name intersectin 1 (SH3 domain protein 1A)
Gene symbol Itsn1
Other names/aliases AA517634
AA545208
AI316805
AI839402
AI848451
Ehsh1
Ese1
Itsn
Sh3p17
Species Mus musculus
 Database cross references - Itsn1
ExoCarta ExoCarta_16443
Vesiclepedia VP_16443
Entrez Gene 16443
UniProt Q9Z0R4  
 Itsn1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Itsn1
Molecular Function
    protein complex scaffold GO:0032947 ISO
    calcium ion binding GO:0005509 IEA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    proline-rich region binding GO:0070064 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    kinase activator activity GO:0019209 IDA
Biological Process
    positive regulation of catalytic activity GO:0043085 IDA
    endocytosis GO:0006897 IEA
    small GTPase mediated signal transduction GO:0007264 IDA
    positive regulation of phosphorylation GO:0042327 IDA
    intracellular signal transduction GO:0035556 IEA
    positive regulation of protein kinase B signaling GO:0051897 IDA
    negative regulation of neuron apoptotic process GO:0043524 IMP
    regulation of Rho protein signal transduction GO:0035023 IEA
Subcellular Localization
    synapse GO:0045202 IEA
    terminal bouton GO:0043195 ISO
    plasma membrane GO:0005886 ISO
    cell junction GO:0030054 IEA
    neuron projection GO:0043005 IEA
    endocytic vesicle GO:0030139 IDA
    cell projection GO:0042995 IEA
    coated pit GO:0005905 ISO
    membrane GO:0016020 IEA
    lamellipodium GO:0030027 IDA
 Experiment description of studies that identified Itsn1 in exosomes
1
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Itsn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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