Gene description for Dpysl2
Gene name dihydropyrimidinase-like 2
Gene symbol Dpysl2
Other names/aliases AI851130
Crmp2
DRP2
Musunc33
TOAD-64
Ulip2
Species Mus musculus
 Database cross references - Dpysl2
ExoCarta ExoCarta_12934
Vesiclepedia VP_12934
Entrez Gene 12934
UniProt O08553  
 Dpysl2 identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dpysl2
Molecular Function
    dihydropyrimidinase activity GO:0004157 IBA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides GO:0016812 IBA
    protein kinase binding GO:0019901 IPI
    identical protein binding GO:0042802 ISO
Biological Process
    pyrimidine nucleobase catabolic process GO:0006208 IBA
    endocytosis GO:0006897 ISO
    endocytosis GO:0006897 ISS
    cytoskeleton organization GO:0007010 IDA
    nervous system development GO:0007399 IEA
    regulation of neuron projection development GO:0010975 IGI
    positive regulation of glutamate secretion GO:0014049 ISO
    cell differentiation GO:0030154 IEA
    regulation of neuron differentiation GO:0045664 ISO
    synaptic vesicle transport GO:0048489 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IEP
    postsynaptic density GO:0014069 IMP
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    axon GO:0030424 IDA
    dendrite GO:0030425 IDA
    dendrite GO:0030425 ISO
    growth cone GO:0030426 ISO
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    terminal bouton GO:0043195 ISO
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 ISO
    synapse GO:0045202 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IEP
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Dpysl2 in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dpysl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Pde4dip  
Affinity Capture-MS Mus musculus
3 Prnp 19122
Affinity Capture-MS Mus musculus
4 Aph1b  
Affinity Capture-MS Mus musculus
5 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
6 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
8 Fmr1  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Dlg4  
Affinity Capture-MS Mus musculus
11 Numb  
Affinity Capture-Western Mus musculus
12 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
13 HSD17B10 3028
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 Huwe1  
Affinity Capture-MS Mus musculus
15 Itch 16396
Affinity Capture-Western Mus musculus
16 Kcnma1  
Affinity Capture-MS Mus musculus
17 Mt3  
Affinity Capture-MS Mus musculus
18 Scn9a  
Affinity Capture-Western Mus musculus
19 Nckap1 50884
Affinity Capture-MS Mus musculus
20 Herc2  
Affinity Capture-MS Mus musculus
21 Cyfip1 20430
Affinity Capture-MS Mus musculus
22 MAPT  
Reconstituted Complex Homo sapiens
23 Agap2  
Affinity Capture-MS Mus musculus
24 LRRK2 120892
Biochemical Activity Homo sapiens
25 Aph1a 226548
Affinity Capture-MS Mus musculus
26 Unk  
Affinity Capture-RNA Mus musculus
27 Nedd4l 83814
Affinity Capture-Western Mus musculus
28 Eps15 13858
Affinity Capture-Western Mus musculus
29 Atxn1  
Proximity Label-MS Mus musculus
30 Fancd2  
Affinity Capture-MS Mus musculus
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