Gene description for Dpysl2
Gene name dihydropyrimidinase-like 2
Gene symbol Dpysl2
Other names/aliases AI851130
Crmp2
DRP2
Musunc33
TOAD-64
Ulip2
Species Mus musculus
 Database cross references - Dpysl2
ExoCarta ExoCarta_12934
Vesiclepedia VP_12934
Entrez Gene 12934
UniProt O08553  
 Dpysl2 identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dpysl2
Molecular Function
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    regulation of neuron differentiation GO:0045664 ISO
    regulation of neuron projection development GO:0010975 IGI
    synaptic vesicle transport GO:0048489 ISO
    cytoskeleton organization GO:0007010 IDA
    multicellular organismal development GO:0007275 IEA
    cell differentiation GO:0030154 IEA
    positive regulation of glutamate secretion GO:0014049 ISO
    endocytosis GO:0006897 ISO
    nervous system development GO:0007399 IEA
Subcellular Localization
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 ISO
    dendrite GO:0030425 ISO
    cytoplasm GO:0005737 IEA
    terminal bouton GO:0043195 ISO
    axon GO:0030424 IDA
    microtubule GO:0005874 ISO
    myelin sheath GO:0043209 ISO
    cytoskeleton GO:0005856 IEA
    extracellular exosome GO:0070062 ISO
    mitochondrion GO:0005739 IDA
    protein complex GO:0043234 ISO
    growth cone GO:0030426 ISO
    membrane GO:0016020 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Dpysl2 in exosomes
1
Experiment ID 26
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 33
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dpysl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dpysl2 is involved
PathwayEvidenceSource
CRMPs in Sema3A signaling IEA Reactome
Recycling pathway of L1 IEA Reactome





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