Gene description for Itch
Gene name itchy, E3 ubiquitin protein ligase
Gene symbol Itch
Other names/aliases 6720481N21Rik
8030492O04Rik
A130065M08
AIP4
C230047C07Rik
Species Mus musculus
 Database cross references - Itch
ExoCarta ExoCarta_16396
Vesiclepedia VP_16396
Entrez Gene 16396
UniProt Q8C863  
 Itch identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itch
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IMP
    ubiquitin-ubiquitin ligase activity GO:0034450 IEA
    ribonucleoprotein complex binding GO:0043021 IEA
    ribonucleoprotein complex binding GO:0043021 ISO
    ubiquitin-like protein ligase binding GO:0044389 IEA
    ubiquitin-like protein ligase binding GO:0044389 ISO
    CXCR chemokine receptor binding GO:0045236 IEA
    CXCR chemokine receptor binding GO:0045236 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 ISA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
    arrestin family protein binding GO:1990763 IEA
    arrestin family protein binding GO:1990763 ISO
Biological Process
    protein polyubiquitination GO:0000209 IDA
    positive regulation of T cell anergy GO:0002669 IMP
    T cell anergy GO:0002870 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 ISA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    protein monoubiquitination GO:0006513 IEA
    protein monoubiquitination GO:0006513 ISO
    apoptotic process GO:0006915 IEA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    protein catabolic process GO:0030163 IMP
    receptor internalization GO:0031623 IEA
    receptor internalization GO:0031623 ISO
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    protein K29-linked ubiquitination GO:0035519 ISO
    protein K29-linked ubiquitination GO:0035519 ISS
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IMP
    negative regulation of cytoplasmic pattern recognition receptor signaling pathway GO:0039532 IEA
    negative regulation of cytoplasmic pattern recognition receptor signaling pathway GO:0039532 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    innate immune response GO:0045087 IEA
    positive regulation of protein catabolic process GO:0045732 IDA
    positive regulation of protein catabolic process GO:0045732 IMP
    negative regulation of JNK cascade GO:0046329 IMP
    negative regulation of defense response to virus GO:0050687 IMP
    negative regulation of defense response to virus GO:0050687 ISO
    defense response to virus GO:0051607 IEA
    protein autoubiquitination GO:0051865 IEA
    protein autoubiquitination GO:0051865 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
    protein K48-linked ubiquitination GO:0070936 IMP
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K48-linked ubiquitination GO:0070936 ISS
    regulation of protein deubiquitination GO:0090085 IMP
    protein branched polyubiquitination GO:0141198 ISS
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IMP
    positive regulation of receptor catabolic process GO:2000646 IEA
    positive regulation of receptor catabolic process GO:2000646 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 IDA
    membrane GO:0016020 IDA
    cytoplasmic vesicle GO:0031410 IDA
    cytoplasmic vesicle GO:0031410 ISO
    early endosome membrane GO:0031901 IEA
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Itch in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itch
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf11  
Affinity Capture-Western Mus musculus
2 ARFGEF2 10564
Reconstituted Complex Homo sapiens
3 Ldlrad3 241576
Affinity Capture-Western Mus musculus
4 Smad2  
Affinity Capture-Western Mus musculus
5 Traf2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Cyld  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Dpysl2 12934
Affinity Capture-Western Mus musculus
8 Tab1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
9 UBE2L3 7332
Reconstituted Complex Homo sapiens
10 Ripk1  
Affinity Capture-Western Mus musculus
11 Fmr1  
Affinity Capture-MS Mus musculus
12 GLIS3  
Two-hybrid Homo sapiens
13 Notch1  
Biochemical Activity Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
14 Tax1bp1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 Rorc  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
16 GLI1  
Affinity Capture-Western Homo sapiens
17 Dzip3  
Co-fractionation Mus musculus
18 Gli1  
Affinity Capture-Western Mus musculus
19 Jun  
Affinity Capture-Western Mus musculus
20 Usp12  
Co-fractionation Mus musculus
21 Cstf1  
Co-fractionation Mus musculus
22 Wwp1  
Co-fractionation Mus musculus
23 Traf6  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
24 Tollip 54473
Co-fractionation Mus musculus
25 LDLRAD3  
Reconstituted Complex Homo sapiens
26 Rpap3  
Co-fractionation Mus musculus
27 Mavs  
Affinity Capture-Western Mus musculus
28 Junb  
Affinity Capture-Western Mus musculus
29 Cxcr4 12767
Affinity Capture-Western Mus musculus
30 Scnn1b  
Co-fractionation Mus musculus
31 NOTCH1 4851
Affinity Capture-Western Homo sapiens
32 Cflar  
Reconstituted Complex Mus musculus
33 Ptpn11 19247
Affinity Capture-Western Mus musculus
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