Gene description for Itch
Gene name itchy, E3 ubiquitin protein ligase
Gene symbol Itch
Other names/aliases 6720481N21Rik
8030492O04Rik
A130065M08
AIP4
C230047C07Rik
Species Mus musculus
 Database cross references - Itch
ExoCarta ExoCarta_16396
Entrez Gene 16396
UniProt Q8C863  
 Itch identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Itch
Molecular Function
    protein-glycine ligase activity, initiating GO:0070736 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    ligase activity GO:0016874 IMP
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    ubiquitin protein ligase activity GO:0061630 ISA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    tubulin-glycine ligase activity GO:0070738 IEA
    protein-glycine ligase activity GO:0070735 IEA
    protein binding GO:0005515 IPI
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    CXCR chemokine receptor binding GO:0045236 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    ubiquitin-protein transferase activity GO:0004842 ISO
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    positive regulation of protein catabolic process GO:0045732 IMP
    negative regulation of defense response to virus GO:0050687 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISA
    negative regulation of alpha-beta T cell proliferation GO:0046642 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K29-linked ubiquitination GO:0035519 ISO
    apoptotic process GO:0006915 IEA
    protein ubiquitination GO:0016567 ISO
    positive regulation of T cell anergy GO:0002669 IMP
    negative regulation of JNK cascade GO:0046329 IMP
    innate immune response GO:0045087 IEA
    protein K63-linked ubiquitination GO:0070534 ISO
    immune system process GO:0002376 IEA
    defense response to virus GO:0051607 IEA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    regulation of protein deubiquitination GO:0090085 IMP
    protein polyubiquitination GO:0000209 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISA
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    cytoplasmic vesicle GO:0031410 IDA
    cell cortex GO:0005938 IDA
    plasma membrane GO:0005886 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Itch in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Itch
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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