Gene description for Tollip
Gene name toll interacting protein
Gene symbol Tollip
Other names/aliases 4930403G24Rik
4931428G15Rik
Species Mus musculus
 Database cross references - Tollip
ExoCarta ExoCarta_54473
Vesiclepedia VP_54473
Entrez Gene 54473
UniProt Q9QZ06  
 Tollip identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tollip
Molecular Function
    interleukin-1, type I receptor binding GO:0005150 IEA
    interleukin-1, type I receptor binding GO:0005150 ISO
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    ubiquitin conjugating enzyme binding GO:0031624 IEA
    ubiquitin conjugating enzyme binding GO:0031624 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    SUMO binding GO:0032183 IEA
    SUMO binding GO:0032183 ISO
    Toll-like receptor binding GO:0035325 IEA
    Toll-like receptor binding GO:0035325 ISO
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IEA
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    autophagy GO:0006914 IEA
    inflammatory response GO:0006954 IEA
    signal transduction GO:0007165 ISO
    phosphorylation GO:0016310 ISO
    phosphorylation GO:0016310 ISS
    epithelial cell differentiation GO:0030855 IEA
    positive regulation of protein sumoylation GO:0033235 IEA
    positive regulation of protein sumoylation GO:0033235 ISO
    protein localization to endosome GO:0036010 IEA
    protein localization to endosome GO:0036010 ISO
    innate immune response GO:0045087 IEA
    interleukin-1-mediated signaling pathway GO:0070498 IDA
    interleukin-1-mediated signaling pathway GO:0070498 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Tollip in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tollip
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Apeh 235606
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
3 Tom1 21968
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Irak1  
Affinity Capture-Western Mus musculus
6 Tom1l2  
Co-fractionation Mus musculus
7 Htt  
Co-localization Mus musculus
8 Fth1 14319
Co-fractionation Mus musculus
9 Itch 16396
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Il1rap 16180
Co-fractionation Mus musculus
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