|
 Gene description for Krt5 |
| Gene name |
keratin 5 |
| Gene symbol |
Krt5 |
| Other names/aliases |
3300001P10Rik
AW146334
CK5
K5
Krt2-5
Tfip8 |
| Species |
Mus musculus |
| Database cross references - Krt5 |
| ExoCarta |
ExoCarta_110308 |
| Entrez Gene |
110308 |
| UniProt |
Q922U2
|
| Krt5 identified in exosomes derived from the following tissue/cell type |
|
Mast cells
|
17486113
|
|
Oligodendrocytes
|
21136642
|
|
Pancreatic cells
|
19351151
|
| Gene ontology annotations for Krt5 |
|
|
| Experiment description of studies that identified Krt5 in exosomes |
| 1 |
| Experiment ID |
15 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✘
|
EV Cytosolic markers |
|
✔
CD63
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 2 |
| Experiment ID |
33 |
| ISEV standards |
|
✔
EM|IEM
|
EV Biophysical techniques |
|
✔
Alix|TSG101
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21136642
|
| Organism |
Mus musculus |
| Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
| Authors |
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J |
| Journal name |
PROTEOMICS_CL
|
| Publication year |
2007 |
| Sample |
Oligodendrocytes |
| Sample name |
Oligodendrocytes
Oli-neu |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy |
|
|
| 3 |
| Experiment ID |
188 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
GAPDH|UCHL1|HSP90
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19351151
|
| Organism |
Mus musculus |
| Experiment description |
Characterization of vesicles secreted from insulinoma NIT-1 cells. |
| Authors |
Lee HS, Jeong J, Lee KJ. |
| Journal name |
J Proteome Res
|
| Publication year |
2009 |
| Sample |
Pancreatic cells |
| Sample name |
Pancreatic beta cell (NIT-1) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for Krt5 |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
|
No interactions are found.
|
|
| Pathways in which Krt5 is involved |
|
|
|
