Gene description for Prkca
Gene name protein kinase C, alpha
Gene symbol Prkca
Other names/aliases AI875142
Pkca
Species Mus musculus
 Database cross references - Prkca
ExoCarta ExoCarta_18750
Entrez Gene 18750
UniProt P20444  
 Prkca identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Prkca
Molecular Function
    protein serine/threonine kinase activity GO:0004674 ISO
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 ISO
    ATP binding GO:0005524 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    nucleotide binding GO:0000166 IEA
    metal ion binding GO:0046872 IEA
    protein kinase activity GO:0004672 ISO
    transferase activity GO:0016740 IEA
    kinase activity GO:0016301 IEA
    protein kinase C activity GO:0004697 ISO
    histone kinase activity (H3-T6 specific) GO:0035403 ISO
    calcium-dependent protein kinase C activity GO:0004698 ISO
Biological Process
    positive regulation of synapse assembly GO:0051965 ISO
    apoptotic process GO:0006915 IEA
    negative regulation of glial cell apoptotic process GO:0034351 ISO
    negative regulation of heart contraction GO:0045822 ISO
    positive regulation of dense core granule biogenesis GO:2000707 IMP
    phosphorylation GO:0016310 IEA
    central nervous system neuron axonogenesis GO:0021955 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    positive regulation of angiogenesis GO:0045766 ISO
    negative regulation of cell proliferation GO:0008285 IGI
    negative regulation of glucose import GO:0046325 IMP
    intrinsic apoptotic signaling pathway GO:0097193 IMP
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISO
    response to ethanol GO:0045471 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    cellular response to carbohydrate stimulus GO:0071322 IDA
    negative regulation of protein phosphorylation GO:0001933 IMP
    learning or memory GO:0007611 ISO
    response to estradiol GO:0032355 ISO
    cellular calcium ion homeostasis GO:0006874 IMP
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 ISO
    positive regulation of endothelial cell migration GO:0010595 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    regulation of muscle contraction GO:0006937 IMP
    regulation of platelet aggregation GO:0090330 ISO
    chondrocyte differentiation GO:0002062 IDA
    angiogenesis GO:0001525 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    peptidyl-serine autophosphorylation GO:0036289 IDA
    positive regulation of protein phosphorylation GO:0001934 IMP
    induction of positive chemotaxis GO:0050930 IMP
    response to interleukin-1 GO:0070555 ISO
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IMP
    histone H3-T6 phosphorylation GO:0035408 ISO
    protein phosphorylation GO:0006468 ISO
    intracellular signal transduction GO:0035556 ISO
    positive regulation of exocytosis GO:0045921 ISO
    regulation of the force of heart contraction GO:0002026 IMP
    positive regulation of inflammatory response GO:0050729 IMP
    negative regulation of translation GO:0017148 ISO
    neutrophil chemotaxis GO:0030593 IMP
    regulation of receptor-mediated endocytosis GO:0048259 ISO
    inactivation of MAPK activity GO:0000188 IMP
    desmosome assembly GO:0002159 ISO
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell adhesion GO:0045785 ISO
    positive regulation of mitotic cell cycle GO:0045931 ISO
    cell adhesion GO:0007155 IEA
    positive regulation of macrophage differentiation GO:0045651 IMP
    response to reactive oxygen species GO:0000302 ISO
    negative regulation of protein kinase activity GO:0006469 IMP
    protein autophosphorylation GO:0046777 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    plasma membrane GO:0005886 ISO
    cytosol GO:0005829 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cell GO:0005623 IMP
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    intracellular GO:0005622 IMP
    nucleus GO:0005634 IDA
    apical part of cell GO:0045177 IDA
    protein complex GO:0043234 ISO
    membrane raft GO:0045121 ISO
    dendrite GO:0030425 IDA
    membrane GO:0016020 IDA
    mitochondrion GO:0005739 ISO
    neuronal cell body GO:0043025 IDA
    neuron projection GO:0043005 ISO
    photoreceptor outer segment GO:0001750 IDA
 Experiment description of studies that identified Prkca in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Prkca
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkca is involved
No pathways found





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