Gene description for Jup
Gene name junction plakoglobin
Gene symbol Jup
Other names/aliases Ctnng
D930025P04Rik
PG
Species Mus musculus
 Database cross references - Jup
ExoCarta ExoCarta_16480
Vesiclepedia VP_16480
Entrez Gene 16480
UniProt Q02257  
 Jup identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Jup
Molecular Function
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    protein binding GO:0005515 IPI
    nuclear receptor binding GO:0016922 IBA
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 IBA
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    protein-containing complex binding GO:0044877 ISO
    alpha-catenin binding GO:0045294 IBA
    alpha-catenin binding GO:0045294 IPI
    alpha-catenin binding GO:0045294 ISO
    cadherin binding GO:0045296 IBA
    cadherin binding GO:0045296 IEA
    cadherin binding GO:0045296 ISO
    cell adhesion molecule binding GO:0050839 ISO
    cytoskeletal protein-membrane anchor activity GO:0106006 IEA
    cytoskeletal protein-membrane anchor activity GO:0106006 ISO
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    positive regulation of cell-matrix adhesion GO:0001954 IEA
    positive regulation of cell-matrix adhesion GO:0001954 ISO
    desmosome assembly GO:0002159 IMP
    desmosome assembly GO:0002159 ISO
    cell adhesion GO:0007155 IDA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    regulation of cell population proliferation GO:0042127 IEA
    regulation of cell population proliferation GO:0042127 ISO
    positive regulation of protein import into nucleus GO:0042307 IEA
    positive regulation of protein import into nucleus GO:0042307 ISO
    negative regulation of blood vessel endothelial cell migration GO:0043537 IEA
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    skin development GO:0043588 IMP
    positive regulation of angiogenesis GO:0045766 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    detection of mechanical stimulus GO:0050982 IEA
    detection of mechanical stimulus GO:0050982 ISO
    positive regulation of DNA-binding transcription factor activity GO:0051091 ISO
    canonical Wnt signaling pathway GO:0060070 IBA
    cellular response to indole-3-methanol GO:0071681 IEA
    cellular response to indole-3-methanol GO:0071681 ISO
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    bundle of His cell-Purkinje myocyte adhesion involved in cell communication GO:0086073 IEA
    bundle of His cell-Purkinje myocyte adhesion involved in cell communication GO:0086073 ISO
    regulation of heart rate by cardiac conduction GO:0086091 IEA
    regulation of heart rate by cardiac conduction GO:0086091 ISO
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 IMP
    cell-cell adhesion GO:0098609 ISO
    regulation of ventricular cardiac muscle cell action potential GO:0098911 IEA
    regulation of ventricular cardiac muscle cell action potential GO:0098911 ISO
Subcellular Localization
    cornified envelope GO:0001533 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    intermediate filament GO:0005882 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    adherens junction GO:0005912 ISO
    fascia adherens GO:0005916 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    intercalated disc GO:0014704 IEA
    intercalated disc GO:0014704 ISO
    actin cytoskeleton GO:0015629 ISO
    apicolateral plasma membrane GO:0016327 ISO
    lateral plasma membrane GO:0016328 ISO
    catenin complex GO:0016342 IBA
    catenin complex GO:0016342 IDA
    catenin complex GO:0016342 ISO
    Z disc GO:0030018 IDA
    desmosome GO:0030057 IDA
    desmosome GO:0030057 IMP
    desmosome GO:0030057 ISO
    protein-DNA complex GO:0032993 IEA
    protein-DNA complex GO:0032993 ISO
    gamma-catenin-TCF7L2 complex GO:0071665 IEA
    gamma-catenin-TCF7L2 complex GO:0071665 ISO
 Experiment description of studies that identified Jup in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Jup
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cep290  
Affinity Capture-MS Mus musculus
2 PRR14L 253143
Affinity Capture-MS Homo sapiens
3 PDCD6 10016
Affinity Capture-MS Homo sapiens
4 APC  
Affinity Capture-MS Homo sapiens
5 BDP1  
Affinity Capture-MS Homo sapiens
6 Gja1 14609
Affinity Capture-Western Mus musculus
7 USP7 7874
Affinity Capture-MS Homo sapiens
8 DHCR24 1718
Affinity Capture-MS Homo sapiens
9 FGD6  
Affinity Capture-MS Homo sapiens
10 GAR1 54433
Affinity Capture-MS Homo sapiens
11 Insr  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 ABCG1  
Affinity Capture-MS Homo sapiens
13 XAB2 56949
Affinity Capture-MS Homo sapiens
14 Pik3r1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 HIGD1A 25994
Affinity Capture-MS Homo sapiens
16 Ctnnd1 12388
Co-fractionation Mus musculus
17 ZC3H14  
Affinity Capture-MS Homo sapiens
18 Pcp2  
Affinity Capture-Western Mus musculus
19 PPIB 5479
Affinity Capture-MS Homo sapiens
20 Kctd13  
Affinity Capture-MS Mus musculus
21 PRPF8 10594
Affinity Capture-MS Homo sapiens
22 Tfe3  
Affinity Capture-MS Mus musculus
23 CCDC84 338657
Affinity Capture-MS Homo sapiens
24 RAB14 51552
Affinity Capture-MS Homo sapiens
25 DMAP1 55929
Affinity Capture-MS Homo sapiens
26 Mks1  
Affinity Capture-MS Mus musculus
27 Iqcb1  
Affinity Capture-MS Mus musculus
28 PRPF40A 55660
Affinity Capture-MS Homo sapiens
29 Ctnna1 12385
Co-fractionation Mus musculus
Two-hybrid Mus musculus
30 SMU1 55234
Affinity Capture-MS Homo sapiens
31 PUS1 80324
Affinity Capture-MS Homo sapiens
32 AKR1B10 57016
Affinity Capture-MS Homo sapiens
33 Frmd5  
Co-fractionation Mus musculus
34 Nphp4  
Affinity Capture-MS Mus musculus
35 TPD52 7163
Affinity Capture-MS Homo sapiens
36 Ptprq  
Affinity Capture-Western Mus musculus
37 TXN 7295
Affinity Capture-MS Homo sapiens
38 WDR35 57539
Affinity Capture-MS Homo sapiens
39 CGRRF1  
Affinity Capture-MS Homo sapiens
40 DHX30 22907
Affinity Capture-MS Homo sapiens
41 ATP6V1A 523
Affinity Capture-MS Homo sapiens
42 TBL1XR1 79718
Affinity Capture-MS Homo sapiens
43 EVPL 2125
Affinity Capture-MS Homo sapiens
44 PRSS1 5644
Affinity Capture-MS Homo sapiens
45 DSC2 1824
Affinity Capture-MS Homo sapiens
46 Cdh2 12558
Affinity Capture-Western Mus musculus
47 CTNNA1 1495
Affinity Capture-MS Homo sapiens
48 MRPS17 51373
Affinity Capture-MS Homo sapiens
49 CHMP2B 25978
Affinity Capture-MS Homo sapiens
50 Ahi1  
Affinity Capture-MS Mus musculus
51 EFTUD2 9343
Affinity Capture-MS Homo sapiens
52 ARID5B 84159
Affinity Capture-MS Homo sapiens
53 SCAMP3 10067
Affinity Capture-MS Homo sapiens
54 CAMK2G 818
Affinity Capture-MS Homo sapiens
55 DSG2 1829
Affinity Capture-MS Homo sapiens
56 SRRT 51593
Affinity Capture-MS Homo sapiens
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