Gene description for XAB2
Gene name XPA binding protein 2
Gene symbol XAB2
Other names/aliases HCNP
HCRN
NTC90
SYF1
Species Homo sapiens
 Database cross references - XAB2
ExoCarta ExoCarta_56949
Vesiclepedia VP_56949
Entrez Gene 56949
HGNC 14089
MIM 610850
UniProt Q9HCS7  
 XAB2 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for XAB2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    generation of catalytic spliceosome for first transesterification step GO:0000349 IBA
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    blastocyst development GO:0001824 IEA
    transcription-coupled nucleotide-excision repair GO:0006283 IDA
    DNA-templated transcription GO:0006351 IDA
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    nucleus GO:0005634 IC
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    U2-type catalytic step 2 spliceosome GO:0071007 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    post-mRNA release spliceosomal complex GO:0071014 IBA
 Experiment description of studies that identified XAB2 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for XAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC12  
Affinity Capture-MS Homo sapiens
2 RALY 22913
Affinity Capture-MS Homo sapiens
3 NUPL2 11097
Affinity Capture-MS Homo sapiens
4 RALYL 138046
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HNRNPC 3183
Affinity Capture-MS Homo sapiens
6 SENP3 26168
Affinity Capture-MS Homo sapiens
7 REC8  
Affinity Capture-MS Homo sapiens
8 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 Prpf8 192159
Affinity Capture-MS Mus musculus
11 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
12 PPIE 10450
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
13 ACTC1 70
Proximity Label-MS Homo sapiens
14 ISY1 57461
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
15 DHX8 1659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 XPA  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 TOP1 7150
Affinity Capture-MS Homo sapiens
18 PRPF3  
Co-fractionation Homo sapiens
19 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
20 DVL3 1857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 AAR2 25980
Affinity Capture-MS Homo sapiens
22 CCDC94  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SYF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ILF2 3608
Affinity Capture-MS Homo sapiens
26 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
27 USB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 KIAA1429 25962
Affinity Capture-MS Homo sapiens
29 DDX23 9416
Co-fractionation Homo sapiens
30 RNPS1 10921
Affinity Capture-MS Homo sapiens
31 CRNKL1 51340
Co-fractionation Homo sapiens
32 Snw1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
33 NFKBIL1  
Affinity Capture-MS Homo sapiens
34 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 Isy1 57905
Affinity Capture-MS Mus musculus
36 SNRPC 6631
Affinity Capture-MS Homo sapiens
37 OBSL1 23363
Affinity Capture-MS Homo sapiens
38 Jup 16480
Affinity Capture-MS Mus musculus
39 SMEK1 55671
Affinity Capture-MS Homo sapiens
40 TMEM192 201931
Affinity Capture-MS Homo sapiens
41 SNRPD3 6634
Affinity Capture-MS Homo sapiens
42 SLX4  
Affinity Capture-MS Homo sapiens
43 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CCDC130  
Affinity Capture-MS Homo sapiens
45 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 Bcas2  
Affinity Capture-MS Mus musculus
47 E2F4  
Affinity Capture-MS Homo sapiens
48 PRPF8 10594
Proximity Label-MS Homo sapiens
49 LIN28B  
Affinity Capture-MS Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 PPIL1 51645
Affinity Capture-MS Homo sapiens
52 GLIS2  
Two-hybrid Homo sapiens
53 SNRNP40 9410
Affinity Capture-MS Homo sapiens
54 SRSF3 6428
Affinity Capture-MS Homo sapiens
55 Crnkl1  
Affinity Capture-MS Mus musculus
56 SIRT7  
Affinity Capture-MS Homo sapiens
57 SRSF6 6431
Affinity Capture-MS Homo sapiens
58 PURG  
Affinity Capture-MS Homo sapiens
59 SNRNP200 23020
Co-fractionation Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 KIAA1143  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PLXNA2 5362
Co-fractionation Homo sapiens
64 PRPF31 26121
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
65 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
66 HSPB8 26353
Affinity Capture-MS Homo sapiens
67 SF3A3 10946
Co-fractionation Homo sapiens
68 SNRPB2 6629
Affinity Capture-MS Homo sapiens
69 PSG11  
Affinity Capture-MS Homo sapiens
70 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SF3B2 10992
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
72 RBM39 9584
Affinity Capture-MS Homo sapiens
73 FANCD2  
Affinity Capture-MS Homo sapiens
74 Ccdc12  
Affinity Capture-MS Mus musculus
75 C9orf78 51759
Affinity Capture-MS Homo sapiens
76 UBE2H 7328
Affinity Capture-MS Homo sapiens
77 BAG2 9532
Affinity Capture-MS Homo sapiens
78 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PLRG1 5356
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
81 CUL3 8452
Affinity Capture-MS Homo sapiens
82 DNAJC17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
84 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ZC3H11A  
Affinity Capture-MS Homo sapiens
86 SUZ12  
Affinity Capture-MS Homo sapiens
87 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
88 SF3A1 10291
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
89 EFTUD2 9343
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
90 TADA2A  
Affinity Capture-MS Homo sapiens
91 PRPF19 27339
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
92 RPA3 6119
Proximity Label-MS Homo sapiens
93 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 EGFR 1956
Negative Genetic Homo sapiens
95 PABPC5 140886
Affinity Capture-MS Homo sapiens
96 METTL21B  
Affinity Capture-MS Homo sapiens
97 DDRGK1 65992
Affinity Capture-MS Homo sapiens
98 RNF2  
Affinity Capture-MS Homo sapiens
99 SF3B3 23450
Co-fractionation Homo sapiens
100 AQR 9716
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
101 POLR2A 5430
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
103 ERCC6  
Affinity Capture-Western Homo sapiens
104 DHX15 1665
Co-fractionation Homo sapiens
105 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
106 EAPP  
Affinity Capture-MS Homo sapiens
107 MAGOH 4116
Affinity Capture-MS Homo sapiens
108 BCAS2 10286
Affinity Capture-MS Homo sapiens
109 ILF3 3609
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
110 CPSF6 11052
Affinity Capture-MS Homo sapiens
111 Cdc5l 71702
Affinity Capture-MS Mus musculus
112 WWOX 51741
Affinity Capture-MS Homo sapiens
113 ERCC8  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
114 MEN1 4221
Affinity Capture-MS Homo sapiens
115 SNRPA 6626
Affinity Capture-MS Homo sapiens
116 CDC40  
Co-fractionation Homo sapiens
117 EIF4A3 9775
Affinity Capture-MS Homo sapiens
118 KIFAP3 22920
Affinity Capture-MS Homo sapiens
119 NUP50 10762
Proximity Label-MS Homo sapiens
120 IK 3550
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
121 DHX16 8449
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
122 SRSF7 6432
Affinity Capture-MS Homo sapiens
123 ZCCHC10  
Affinity Capture-MS Homo sapiens
124 CIT 11113
Affinity Capture-MS Homo sapiens
125 LDLR 3949
Positive Genetic Homo sapiens
126 SNW1 22938
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
127 KRAS 3845
Negative Genetic Homo sapiens
128 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 AR 367
Proximity Label-MS Homo sapiens
130 RC3H1 149041
Affinity Capture-MS Homo sapiens
131 SNRPA1 6627
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 PRMT1 3276
Affinity Capture-MS Homo sapiens
133 ZNF830  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 EP300 2033
Affinity Capture-MS Homo sapiens
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