Gene description for Prpf8
Gene name pre-mRNA processing factor 8
Gene symbol Prpf8
Other names/aliases AU019467
D11Bwg0410e
DBF3/PRP8
Prp8
Sfprp8l
Species Mus musculus
 Database cross references - Prpf8
ExoCarta ExoCarta_192159
Entrez Gene 192159
UniProt Q99PV0  
 Prpf8 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Pancreatic cells 19351151    
 Gene ontology annotations for Prpf8
Molecular Function
    second spliceosomal transesterification activity GO:0000386 IBA
    nucleic acid binding GO:0003676 IEA
    K63-linked polyubiquitin binding GO:0070530 ISO
    U5 snRNA binding GO:0030623 IBA
    U2 snRNA binding GO:0030620 IBA
    U6 snRNA binding GO:0017070 IBA
    poly(A) RNA binding GO:0044822 ISO
    RNA binding GO:0003723 IEA
    pre-mRNA intronic binding GO:0097157 IBA
    U1 snRNA binding GO:0030619 IBA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    RNA splicing GO:0008380 IEA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA processing GO:0006397 IEA
Subcellular Localization
    spliceosomal complex GO:0005681 IEA
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
    ribonucleoprotein complex GO:0030529 IEA
    U5 snRNP GO:0005682 ISO
 Experiment description of studies that identified Prpf8 in exosomes
1
Experiment ID 263
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prpf8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prpf8 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway IEA Reactome
mRNA Splicing - Minor Pathway IEA Reactome





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