Gene description for Eif4a3
Gene name eukaryotic translation initiation factor 4A3
Gene symbol Eif4a3
Other names/aliases 2400003O03Rik
Ddx48
eIF4A-III
mKIAA0111
Species Mus musculus
 Database cross references - Eif4a3
ExoCarta ExoCarta_192170
Entrez Gene 192170
UniProt Q91VC3  
 Eif4a3 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Eif4a3
Molecular Function
    ATP binding GO:0005524 ISO
    ATP-dependent RNA helicase activity GO:0004004 ISO
    mRNA binding GO:0003729 ISO
    poly(A) RNA binding GO:0044822 ISO
    poly(A) binding GO:0008143 ISO
Biological Process
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    RNA secondary structure unwinding GO:0010501 IBA
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    negative regulation of translation GO:0017148 ISO
    RNA splicing GO:0008380 IBA
    positive regulation of translation GO:0045727 ISO
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISO
Subcellular Localization
    catalytic step 2 spliceosome GO:0071013 ISO
    exon-exon junction complex GO:0035145 ISO
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eif4a3 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eif4a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eif4a3 is involved
No pathways found





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