Gene description for Hnrnpk
Gene name heterogeneous nuclear ribonucleoprotein K
Gene symbol Hnrnpk
Other names/aliases Hnrpk
KBBP
NOVA
Species Mus musculus
 Database cross references - Hnrnpk
ExoCarta ExoCarta_15387
Entrez Gene 15387
UniProt P61979  
 Hnrnpk identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Hnrnpk
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    core promoter proximal region DNA binding GO:0001159 ISO
    nucleic acid binding GO:0003676 IEA
    DNA binding GO:0003677 IEA
    poly(A) RNA binding GO:0044822 ISO
    mRNA 3'-UTR binding GO:0003730 ISO
    RNA binding GO:0003723 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    protein binding GO:0005515 IPI
    single-stranded DNA binding GO:0003697 ISO
Biological Process
    positive regulation of low-density lipoprotein particle receptor biosynthetic process GO:0045716 ISO
    regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter GO:0072369 ISO
    RNA splicing GO:0008380 IEA
    positive regulation of protein localization to cell surface GO:2000010 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IMP
    positive regulation of dendritic spine development GO:0060999 ISO
    positive regulation of dendrite extension GO:1903861 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    mRNA processing GO:0006397 IEA
    regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902165 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    cellular response to insulin stimulus GO:0032869 ISO
    positive regulation of long-term synaptic potentiation GO:1900273 ISO
    transcription from RNA polymerase II promoter GO:0006366 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    positive regulation of synapse maturation GO:0090129 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    ovarian follicle development GO:0001541 ISO
    cellular response to organic cyclic compound GO:0071407 ISO
    positive regulation of RNA splicing GO:0033120 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    negative regulation of branching morphogenesis of a nerve GO:2000173 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of synaptic transmission GO:0050806 ISO
    regulation of low-density lipoprotein particle clearance GO:0010988 ISO
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of neuron projection development GO:0010976 ISO
Subcellular Localization
    axon terminus GO:0043679 ISO
    cytoplasm GO:0005737 IEA
    cell junction GO:0030054 IEA
    cell projection GO:0042995 IEA
    postsynaptic density GO:0014069 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
    mitochondrion GO:0005739 ISO
    spliceosomal complex GO:0005681 IEA
    extracellular exosome GO:0070062 ISO
    ribonucleoprotein complex GO:0030529 IEA
    focal adhesion GO:0005925 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    nuclear chromatin GO:0000790 ISO
    nucleus GO:0005634 ISO
    dendritic spine GO:0043197 ISO
 Experiment description of studies that identified Hnrnpk in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hnrnpk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hnrnpk is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome





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