Gene description for Hnrnpk
Gene name heterogeneous nuclear ribonucleoprotein K
Gene symbol Hnrnpk
Other names/aliases Hnrpk
KBBP
NOVA
Species Mus musculus
 Database cross references - Hnrnpk
ExoCarta ExoCarta_15387
Vesiclepedia VP_15387
Entrez Gene 15387
UniProt P61979  
 Hnrnpk identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hnrnpk
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 ISO
    DNA binding GO:0003677 IEA
    double-stranded DNA binding GO:0003690 ISO
    single-stranded DNA binding GO:0003697 ISO
    RNA binding GO:0003723 IDA
    mRNA binding GO:0003729 IBA
    mRNA 3'-UTR binding GO:0003730 ISO
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    pre-mRNA 3'-splice site binding GO:0030628 ISO
    heat shock protein binding GO:0031072 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    actinin binding GO:0042805 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    ATPase binding GO:0051117 ISO
    mRNA CDS binding GO:1990715 ISO
    C-rich single-stranded DNA binding GO:1990829 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    ovarian follicle development GO:0001541 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    regulation of low-density lipoprotein particle clearance GO:0010988 IEA
    regulation of low-density lipoprotein particle clearance GO:0010988 ISO
    cerebral cortex development GO:0021987 ISO
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 IEA
    positive regulation of myelination GO:0031643 ISO
    cellular response to insulin stimulus GO:0032869 ISO
    positive regulation of RNA splicing GO:0033120 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    regulation of mRNA splicing, via spliceosome GO:0048024 IBA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IMP
    positive regulation of receptor-mediated endocytosis GO:0048260 IEA
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    modulation of chemical synaptic transmission GO:0050804 ISO
    positive regulation of synaptic transmission GO:0050806 ISO
    random inactivation of X chromosome GO:0060816 IEA
    positive regulation of dendritic spine development GO:0060999 ISO
    positive regulation of synapse maturation GO:0090129 ISO
    regulation of postsynapse organization GO:0099175 ISO
    positive regulation of long-term synaptic potentiation GO:1900273 ISO
    regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902165 IMP
    positive regulation of dendrite extension GO:1903861 ISO
    cellular response to forskolin GO:1904322 ISO
    positive regulation of low-density lipoprotein receptor activity GO:1905599 ISO
    positive regulation of protein localization to cell surface GO:2000010 ISO
    negative regulation of branching morphogenesis of a nerve GO:2000173 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    podosome GO:0002102 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cell cortex GO:0005938 ISO
    cytoplasmic stress granule GO:0010494 IDA
    protein-DNA complex GO:0032993 ISO
    cell projection GO:0042995 IEA
    dendritic spine GO:0043197 ISO
    axon terminus GO:0043679 ISO
    anchoring junction GO:0070161 IEA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpk in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Prpf8 192159
Co-fractionation Mus musculus
5 RALYL 138046
Two-hybrid Homo sapiens
6 Snrnp200 320632
Co-fractionation Mus musculus
7 KHDRBS3  
Two-hybrid Homo sapiens
8 RBM42  
Two-hybrid Homo sapiens
9 SMARCB1 6598
Two-hybrid Homo sapiens
10 IPO5 3843
Two-hybrid Homo sapiens
11 Snrpd1 20641
Co-fractionation Mus musculus
12 Srrm1 51796
Co-fractionation Mus musculus
13 Celf1  
Two-hybrid Mus musculus
14 Fzr1  
Affinity Capture-MS Mus musculus
15 RBM19 9904
Two-hybrid Homo sapiens
16 ZCRB1  
Two-hybrid Homo sapiens
17 Plrg1  
Co-fractionation Mus musculus
18 RBMX 27316
Two-hybrid Homo sapiens
19 Sf3b1 81898
Co-fractionation Mus musculus
20 Srsf7 225027
Two-hybrid Mus musculus
21 DDX17 10521
Two-hybrid Homo sapiens
22 HNRNPLL 92906
Two-hybrid Homo sapiens
23 QKI 9444
Two-hybrid Homo sapiens
24 SNRPA 6626
Two-hybrid Homo sapiens
25 Rbm14  
Two-hybrid Mus musculus
26 YBX2 51087
Two-hybrid Homo sapiens
27 SF1 7536
Two-hybrid Homo sapiens
28 Tfe3  
Affinity Capture-MS Mus musculus
29 Cul3  
Affinity Capture-MS Mus musculus
30 Snrpd2 107686
Co-fractionation Mus musculus
31 HNRNPR 10236
Two-hybrid Homo sapiens
32 Igf2bp3 140488
Two-hybrid Mus musculus
33 Lsm3  
Co-fractionation Mus musculus
34 Kcnma1  
Affinity Capture-MS Mus musculus
35 Snrpd3 67332
Co-fractionation Mus musculus
36 Taf15  
Two-hybrid Mus musculus
37 RBM7  
Two-hybrid Homo sapiens
38 Ywhaq 22630
Co-fractionation Mus musculus
39 Sf3b3 101943
Co-fractionation Mus musculus
40 APOBEC3C 27350
Two-hybrid Homo sapiens
41 Rbm8a  
Co-fractionation Mus musculus
42 Nck2 17974
Two-hybrid Mus musculus
43 KHDRBS2  
Two-hybrid Homo sapiens
44 PRR3  
Two-hybrid Homo sapiens
45 Srsf1 110809
Co-fractionation Mus musculus
46 Hnrnpu 51810
Two-hybrid Mus musculus
47 Ywhab 54401
Co-fractionation Mus musculus
48 Pcbp1 23983
Two-hybrid Mus musculus
49 Srrm2  
Co-fractionation Mus musculus
50 RBM3 5935
Two-hybrid Homo sapiens
51 LPAR1 1902
Two-hybrid Homo sapiens
52 Syncrip 56403
Co-fractionation Mus musculus
53 Snw1  
Co-fractionation Mus musculus
54 Cirbp  
Two-hybrid Mus musculus
55 Ppwd1  
Co-fractionation Mus musculus
56 Grb2 14784
Two-hybrid Mus musculus
57 EXOSC8  
Two-hybrid Homo sapiens
58 SRSF10 10772
Two-hybrid Homo sapiens
59 GANAB 23193
Two-hybrid Homo sapiens
60 ZC3HAV1 56829
Two-hybrid Homo sapiens
61 Eed  
Affinity Capture-MS Mus musculus
62 Cwc27  
Co-fractionation Mus musculus
63 Ptcd3  
Two-hybrid Mus musculus
64 Srsf3 20383
Two-hybrid Mus musculus
65 U2af2 22185
Two-hybrid Mus musculus
66 Mapt  
Affinity Capture-MS Mus musculus
67 Skiv2l2  
Co-fractionation Mus musculus
68 SMU1 55234
Two-hybrid Homo sapiens
69 LARP4 113251
Two-hybrid Homo sapiens
70 Otx2  
Affinity Capture-MS Mus musculus
71 Fancd2  
Affinity Capture-MS Mus musculus
72 Ewsr1 14030
Two-hybrid Mus musculus
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