Gene description for Srsf7
Gene name serine/arginine-rich splicing factor 7
Gene symbol Srsf7
Other names/aliases 35kDa
9430065L19Rik
9G8
NX-96
Sfrs7
Species Mus musculus
 Database cross references - Srsf7
ExoCarta ExoCarta_225027
Vesiclepedia VP_225027
Entrez Gene 225027
UniProt Q8BL97  
 Srsf7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf7
Molecular Function
    RNA binding GO:0003723 IEA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    RNA splicing GO:0008380 ISO
    mRNA cis splicing, via spliceosome GO:0045292 IBA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISS
    mRNA transport GO:0051028 IEA
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IEA
    nuclear speck GO:0016607 IBA
 Experiment description of studies that identified Srsf7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Prpf4b 19134
Co-fractionation Mus musculus
2 PDAP1 11333
Two-hybrid Homo sapiens
3 1110037F02Rik 66185
Co-fractionation Mus musculus
4 Bcas2  
Co-fractionation Mus musculus
5 Tnpo3 320938
Co-fractionation Mus musculus
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Smu1  
Co-fractionation Mus musculus
9 Cwc22  
Co-fractionation Mus musculus
10 Mov10 17454
Co-fractionation Mus musculus
11 Cwc15  
Co-fractionation Mus musculus
12 Thoc2  
Co-fractionation Mus musculus
13 Spen  
Co-fractionation Mus musculus
14 Tfip11  
Co-fractionation Mus musculus
15 Snrpc 20630
Co-fractionation Mus musculus
16 Htatsf1  
Co-fractionation Mus musculus
17 LUC7L2 51631
Two-hybrid Homo sapiens
18 Dhx8  
Co-fractionation Mus musculus
19 Hnrnpk 15387
Two-hybrid Mus musculus
20 Eed  
Affinity Capture-MS Mus musculus
21 Srsf3 20383
Co-fractionation Mus musculus
22 Snrnp70 20637
Co-fractionation Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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