Gene description for Prpf4b
Gene name PRP4 pre-mRNA processing factor 4 homolog B (yeast)
Gene symbol Prpf4b
Other names/aliases 2610037H07
Prp4
Prp4k
Prpk
cbp143
Species Mus musculus
 Database cross references - Prpf4b
ExoCarta ExoCarta_19134
Vesiclepedia VP_19134
Entrez Gene 19134
UniProt Q61136  
 Prpf4b identified in exosomes derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Prpf4b
Molecular Function
    protein kinase activity GO:0004672 ISS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    spliceosomal snRNP assembly GO:0000387 ISO
    spliceosomal snRNP assembly GO:0000387 ISS
    chromatin remodeling GO:0006338 IEA
    positive regulation of hippo signaling GO:0035332 IEA
    positive regulation of hippo signaling GO:0035332 ISO
    mRNA cis splicing, via spliceosome GO:0045292 IEA
    positive regulation of protein export from nucleus GO:0046827 IEA
    positive regulation of protein export from nucleus GO:0046827 ISO
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 ISO
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 ISS
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    chromosome GO:0005694 IDA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Prpf4b in exosomes
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prpf4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Prpf18  
Co-fractionation Mus musculus
4 Prpf8 192159
Co-fractionation Mus musculus
5 Cluh  
Affinity Capture-MS Mus musculus
6 Pou5f1  
Affinity Capture-MS Mus musculus
7 Cactin  
Co-fractionation Mus musculus
8 1110037F02Rik 66185
Co-fractionation Mus musculus
9 Snrnp40  
Co-fractionation Mus musculus
10 Bcas2  
Co-fractionation Mus musculus
11 Srsf7 225027
Co-fractionation Mus musculus
12 Atxn1  
Proximity Label-MS Mus musculus
13 Foxp3  
Affinity Capture-MS Mus musculus
14 Cwc22  
Co-fractionation Mus musculus
15 Prpf3 70767
Co-fractionation Mus musculus
16 Pnn  
Co-fractionation Mus musculus
17 Cwc15  
Co-fractionation Mus musculus
18 Thoc2  
Co-fractionation Mus musculus
19 Ppil4  
Co-fractionation Mus musculus
20 Srrm2  
Co-fractionation Mus musculus
21 Spen  
Co-fractionation Mus musculus
22 Cdk12  
Co-fractionation Mus musculus
23 Snrpc 20630
Co-fractionation Mus musculus
24 Htatsf1  
Co-fractionation Mus musculus
25 Hnrnpm 76936
Co-fractionation Mus musculus
26 Ddx41  
Co-fractionation Mus musculus
27 Prpf40a  
Co-fractionation Mus musculus
28 Srsf3 20383
Co-fractionation Mus musculus
29 Zfp292  
Co-fractionation Mus musculus
30 Snrnp70 20637
Co-fractionation Mus musculus
31 Ik  
Co-fractionation Mus musculus
32 Rbm25  
Co-fractionation Mus musculus
33 Paxbp1  
Co-fractionation Mus musculus
View the network image/svg+xml



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