Gene description for Prpf3
Gene name PRP3 pre-mRNA processing factor 3 homolog (yeast)
Gene symbol Prpf3
Other names/aliases 3632413F13Rik
Species Mus musculus
 Database cross references - Prpf3
ExoCarta ExoCarta_70767
Vesiclepedia VP_70767
Entrez Gene 70767
UniProt Q922U1  
 Prpf3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prpf3
Molecular Function
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Cajal body GO:0015030 IEA
    Cajal body GO:0015030 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    protein-containing complex GO:0032991 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISS
    U2-type precatalytic spliceosome GO:0071005 IEA
    U2-type precatalytic spliceosome GO:0071005 ISO
 Experiment description of studies that identified Prpf3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prpf3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHF6  
Affinity Capture-MS Homo sapiens
2 Ddx5 13207
Co-fractionation Mus musculus
3 Dhx29  
Co-fractionation Mus musculus
4 PAICS 10606
Affinity Capture-MS Homo sapiens
5 Fra10ac1  
Co-fractionation Mus musculus
6 Rpl13a 22121
Co-fractionation Mus musculus
7 Hypk  
Co-fractionation Mus musculus
8 PRPF6 24148
Affinity Capture-MS Homo sapiens
9 Prpf18  
Co-fractionation Mus musculus
10 Zmat2  
Co-fractionation Mus musculus
11 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
12 Prpf8 192159
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Cdc5l 71702
Co-fractionation Mus musculus
15 Rps2 16898
Co-fractionation Mus musculus
16 Sart3  
Co-fractionation Mus musculus
17 H2AFY 9555
Affinity Capture-MS Homo sapiens
18 Snrpd2 107686
Co-fractionation Mus musculus
19 WBP4  
Affinity Capture-MS Homo sapiens
20 Rbm39 170791
Co-fractionation Mus musculus
21 Rpl6 19988
Co-fractionation Mus musculus
22 PRPF4 9128
Affinity Capture-MS Homo sapiens
23 Snrnp200 320632
Co-fractionation Mus musculus
24 Sf3a3 75062
Co-fractionation Mus musculus
25 Prpf19 28000
Co-fractionation Mus musculus
26 Cwc22  
Co-fractionation Mus musculus
27 Rps6 20104
Co-fractionation Mus musculus
28 Prpf4  
Co-fractionation Mus musculus
29 Ppwd1  
Co-fractionation Mus musculus
30 Crnkl1  
Co-fractionation Mus musculus
31 SART1 9092
Affinity Capture-MS Homo sapiens
32 Lrrc59  
Co-fractionation Mus musculus
33 Rpl22 19934
Co-fractionation Mus musculus
34 Cwc15  
Co-fractionation Mus musculus
35 DDX23 9416
Affinity Capture-MS Homo sapiens
36 Gtf2f1  
Co-fractionation Mus musculus
37 RPL38 6169
Affinity Capture-MS Homo sapiens
38 Ctnnbl1  
Co-fractionation Mus musculus
39 Tfip11  
Co-fractionation Mus musculus
40 MRPS2 51116
Affinity Capture-MS Homo sapiens
41 Slu7  
Co-fractionation Mus musculus
42 2310036O22Rik  
Co-fractionation Mus musculus
43 Spen  
Co-fractionation Mus musculus
44 PRPF31 26121
Affinity Capture-MS Homo sapiens
45 Ncbp2  
Co-fractionation Mus musculus
46 RPL31 6160
Affinity Capture-MS Homo sapiens
47 ATXN2L 11273
Affinity Capture-MS Homo sapiens
48 Rps3a1 20091
Co-fractionation Mus musculus
49 NHP2L1 4809
Affinity Capture-MS Homo sapiens
50 Htatsf1  
Co-fractionation Mus musculus
51 MIR3654  
Affinity Capture-MS Homo sapiens
52 SART3 9733
Affinity Capture-MS Homo sapiens
53 Rcsd1  
Co-fractionation Mus musculus
54 Snrpa1 68981
Co-fractionation Mus musculus
55 Rps14 20044
Co-fractionation Mus musculus
56 Smndc1  
Co-fractionation Mus musculus
57 RFC2 5982
Affinity Capture-MS Homo sapiens
58 Prpf40a  
Co-fractionation Mus musculus
59 Cactin  
Co-fractionation Mus musculus
60 RBBP5 5929
Affinity Capture-MS Homo sapiens
61 Prpf4b 19134
Co-fractionation Mus musculus
62 Prpf31  
Co-fractionation Mus musculus
63 Thoc1  
Co-fractionation Mus musculus
64 Txnl4a  
Co-fractionation Mus musculus
65 Fancd2  
Affinity Capture-MS Mus musculus
66 Paxbp1  
Co-fractionation Mus musculus
67 DNAJC13 23317
Affinity Capture-MS Homo sapiens
68 Srsf2 20382
Co-fractionation Mus musculus
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