Gene description for PRPF4
Gene name pre-mRNA processing factor 4
Gene symbol PRPF4
Other names/aliases HPRP4
HPRP4P
PRP4
Prp4p
RP70
SNRNP60
Species Homo sapiens
 Database cross references - PRPF4
ExoCarta ExoCarta_9128
Entrez Gene 9128
HGNC 17349
MIM 607795
UniProt O43172  
 PRPF4 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for PRPF4
Molecular Function
    protein binding GO:0005515 IPI
    U4 snRNA binding GO:0030621 IBA
    U6 snRNA binding GO:0017070 IBA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 TAS
    RNA splicing, via transesterification reactions GO:0000375 NAS
    RNA splicing GO:0008380 TAS
    gene expression GO:0010467 TAS
    RNA processing GO:0006396 TAS
Subcellular Localization
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 snRNP GO:0071001 IDA
    nucleoplasm GO:0005654 TAS
    nuclear speck GO:0016607 IEA
    nucleus GO:0005634 TAS
    spliceosomal complex GO:0005681 NAS
    Cajal body GO:0015030 IDA
 Experiment description of studies that identified PRPF4 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRPF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPIH 10465
Affinity Capture-MS Homo sapiens
2 SART3 9733
FRET Homo sapiens
3 SRSF1 6426
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 CLK1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 ATP5J2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRPF4 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome





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