Gene description for CERK
Gene name ceramide kinase
Gene symbol CERK
Other names/aliases LK4
dA59H18.2
dA59H18.3
hCERK
Species Homo sapiens
 Database cross references - CERK
ExoCarta ExoCarta_64781
Vesiclepedia VP_64781
Entrez Gene 64781
HGNC 19256
MIM 610307
UniProt Q8TCT0  
 CERK identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for CERK
Molecular Function
    magnesium ion binding GO:0000287 IDA
    ceramide kinase activity GO:0001729 IBA
    ceramide kinase activity GO:0001729 IDA
    ceramide kinase activity GO:0001729 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    ceramide metabolic process GO:0006672 IBA
    ceramide metabolic process GO:0006672 IDA
    ceramide metabolic process GO:0006672 TAS
    glycosphingolipid biosynthetic process GO:0006688 TAS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified CERK in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CERK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 WDR46  
Affinity Capture-MS Homo sapiens
4 VWCE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ILKAP 80895
Affinity Capture-MS Homo sapiens
6 GPIHBP1  
Affinity Capture-MS Homo sapiens
7 CCNDBP1 23582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NCBP1 4686
Affinity Capture-MS Homo sapiens
9 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 UBL4A 8266
Affinity Capture-MS Homo sapiens
11 SPG7  
Affinity Capture-MS Homo sapiens
12 TTYH1  
Affinity Capture-MS Homo sapiens
13 GEMIN2 8487
Affinity Capture-MS Homo sapiens
14 GPR156  
Affinity Capture-MS Homo sapiens
15 NR3C1 2908
Affinity Capture-MS Homo sapiens
16 USP1 7398
Affinity Capture-MS Homo sapiens
17 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ALPK2  
Affinity Capture-MS Homo sapiens
19 ALAS1  
Two-hybrid Homo sapiens
20 TRIM66  
Affinity Capture-MS Homo sapiens
21 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TNFRSF10D 8793
Affinity Capture-MS Homo sapiens
23 HLTF  
Affinity Capture-MS Homo sapiens
24 CELF1 10658
Affinity Capture-MS Homo sapiens
25 CX3CL1  
Affinity Capture-MS Homo sapiens
26 BUB1B  
Affinity Capture-MS Homo sapiens
27 MCC 4163
Affinity Capture-MS Homo sapiens
28 PRPF4 9128
Affinity Capture-MS Homo sapiens
29 PRMT1 3276
Affinity Capture-MS Homo sapiens
30 DDX23 9416
Affinity Capture-MS Homo sapiens
31 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
32 KRTAP10-3  
Two-hybrid Homo sapiens
33 KCNE3  
Affinity Capture-MS Homo sapiens
34 PRRC2B  
Affinity Capture-MS Homo sapiens
35 FAXC  
Affinity Capture-MS Homo sapiens
36 HEPACAM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 BAG6 7917
Affinity Capture-MS Homo sapiens
38 FST 10468
Affinity Capture-MS Homo sapiens
39 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
40 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
41 IK 3550
Affinity Capture-MS Homo sapiens
42 RBM26 64062
Affinity Capture-MS Homo sapiens
43 C22orf42  
Affinity Capture-MS Homo sapiens
44 APOL2 23780
Affinity Capture-MS Homo sapiens
45 TBC1D22A  
Affinity Capture-MS Homo sapiens
46 RPRM  
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 UGT1A7 54577
Affinity Capture-MS Homo sapiens
49 WDR43 23160
Affinity Capture-MS Homo sapiens
50 RAD18  
Affinity Capture-MS Homo sapiens
51 U2AF2 11338
Affinity Capture-MS Homo sapiens
52 TNFRSF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 IL20RA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 KCTD17 79734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NOTCH2NL 388677
Two-hybrid Homo sapiens
56 PTGES3 10728
Affinity Capture-MS Homo sapiens
57 BEND3  
Affinity Capture-MS Homo sapiens
58 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SLC31A1 1317
Affinity Capture-MS Homo sapiens
60 PDCD2 5134
Affinity Capture-MS Homo sapiens
61 MTUS2 23281
Two-hybrid Homo sapiens
62 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 CCDC174  
Affinity Capture-MS Homo sapiens
64 TAF13  
Affinity Capture-MS Homo sapiens
65 CIRH1A 84916
Affinity Capture-MS Homo sapiens
66 DGCR2 9993
Affinity Capture-MS Homo sapiens
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