Gene description for N4BP2L2
Gene name NEDD4 binding protein 2-like 2
Gene symbol N4BP2L2
Other names/aliases 92M18.3
CG005
PFAAP5
Species Homo sapiens
 Database cross references - N4BP2L2
ExoCarta ExoCarta_10443
Entrez Gene 10443
HGNC 26916
MIM 615788
UniProt Q92802  
 N4BP2L2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 19056867    
 Gene ontology annotations for N4BP2L2
Molecular Function
    enzyme binding GO:0019899 IPI
    protein binding GO:0005515 IPI
    RNA polymerase II transcription corepressor activity GO:0001106 ISS
    molecular_function GO:0003674 ND
Biological Process
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    negative regulation of hematopoietic stem cell differentiation GO:1902037 IMP
    positive regulation of hematopoietic stem cell proliferation GO:1902035 IMP
Subcellular Localization
    transcriptional repressor complex GO:0017053 IDA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified N4BP2L2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for N4BP2L2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which N4BP2L2 is involved
No pathways found





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