Gene description for EPS15L1
Gene name epidermal growth factor receptor pathway substrate 15-like 1
Gene symbol EPS15L1
Other names/aliases EPS15R
Species Homo sapiens
 Database cross references - EPS15L1
ExoCarta ExoCarta_58513
Vesiclepedia VP_58513
Entrez Gene 58513
HGNC 24634
UniProt Q9UBC2  
 EPS15L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for EPS15L1
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    endocytosis GO:0006897 IBA
    endosomal transport GO:0016197 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    clathrin coat of coated pit GO:0030132 IBA
 Experiment description of studies that identified EPS15L1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EPS15L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAPPC2 6399
Two-hybrid Homo sapiens
2 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SMC3 9126
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KIAA1107  
Affinity Capture-MS Homo sapiens
6 ATG9A 79065
Proximity Label-MS Homo sapiens
7 SYCE1  
Affinity Capture-MS Homo sapiens
8 RIN3  
Affinity Capture-MS Homo sapiens
9 Numb  
Affinity Capture-MS Mus musculus
10 FCHO1  
Affinity Capture-MS Homo sapiens
11 EPS15 2060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 Ndc80  
Affinity Capture-MS Mus musculus
13 RNMT 8731
Co-fractionation Homo sapiens
14 UBC 7316
Reconstituted Complex Homo sapiens
15 TRAPPC2B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 DEGS1  
Affinity Capture-MS Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 RNPS1 10921
Affinity Capture-MS Homo sapiens
19 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
20 MCM2 4171
Affinity Capture-MS Homo sapiens
21 AP2A1 160
Affinity Capture-MS Homo sapiens
22 SPAG9 9043
Affinity Capture-MS Homo sapiens
23 MCM6 4175
Affinity Capture-MS Homo sapiens
24 UBQLN1 29979
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
25 LURAP1L  
Affinity Capture-MS Homo sapiens
26 STX6 10228
Proximity Label-MS Homo sapiens
27 ATG16L1 55054
Affinity Capture-MS Homo sapiens
28 DVL2 1856
Affinity Capture-MS Homo sapiens
29 KIF14 9928
Affinity Capture-MS Homo sapiens
30 PRNP 5621
Affinity Capture-MS Homo sapiens
31 C15orf59  
Affinity Capture-MS Homo sapiens
32 AURKA 6790
Affinity Capture-MS Homo sapiens
33 KIFC1 3833
Affinity Capture-MS Homo sapiens
34 MIB1 57534
Proximity Label-MS Homo sapiens
35 AGFG1 3267
Phenotypic Enhancement Homo sapiens
36 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NECAP2 55707
Affinity Capture-MS Homo sapiens
38 CDH1 999
Proximity Label-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 DCAF7 10238
Affinity Capture-MS Homo sapiens
41 Odf2  
Affinity Capture-MS Mus musculus
42 HLA-DRB5 3127
Affinity Capture-MS Homo sapiens
43 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 AP1B1 162
Affinity Capture-MS Homo sapiens
45 RUNDC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 GAREM  
Affinity Capture-MS Homo sapiens
47 GNG5 2787
Two-hybrid Homo sapiens
48 PHLPP1  
Proximity Label-MS Homo sapiens
49 STON2 85439
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
50 Rab5c 19345
Affinity Capture-MS Mus musculus
51 RNF40 9810
Affinity Capture-MS Homo sapiens
52 HSF1  
Affinity Capture-MS Homo sapiens
53 Zwint  
Affinity Capture-MS Mus musculus
54 CEP63  
Affinity Capture-MS Homo sapiens
55 XPO1 7514
Affinity Capture-MS Homo sapiens
56 USP15 9958
Affinity Capture-MS Homo sapiens
57 MCM4 4173
Affinity Capture-MS Homo sapiens
58 GTF3C4 9329
Affinity Capture-MS Homo sapiens
59 SEC24A 10802
Co-fractionation Homo sapiens
60 NXF1 10482
Affinity Capture-RNA Homo sapiens
61 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 BIRC6 57448
Co-fractionation Homo sapiens
63 Otud5  
Affinity Capture-MS Mus musculus
64 GBF1 8729
Co-fractionation Homo sapiens
65 FCHO2 115548
Affinity Capture-MS Homo sapiens
66 EPN1 29924
Affinity Capture-Western Homo sapiens
67 AP2A2 161
Affinity Capture-MS Homo sapiens
68 SBNO1 55206
Affinity Capture-MS Homo sapiens
69 NDC80 10403
Affinity Capture-MS Homo sapiens
70 SGIP1  
Affinity Capture-MS Homo sapiens
71 CROCC 9696
Affinity Capture-MS Homo sapiens
72 SMC1A 8243
Affinity Capture-MS Homo sapiens
73 AGFG2  
Phenotypic Enhancement Homo sapiens
74 ANK3  
Affinity Capture-MS Homo sapiens
75 NCAPG 64151
Co-fractionation Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 TP53BP2  
Affinity Capture-MS Homo sapiens
78 ITSN1 6453
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 GRB2 2885
Affinity Capture-MS Homo sapiens
80 LSR 51599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 Fcho2  
Affinity Capture-MS Mus musculus
82 BFSP1  
Affinity Capture-MS Homo sapiens
83 ZZEF1 23140
Co-fractionation Homo sapiens
84 SOX4  
Affinity Capture-MS Homo sapiens
85 SOS1 6654
Affinity Capture-MS Homo sapiens
86 PDZD8 118987
Affinity Capture-MS Homo sapiens
87 SPC25 57405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 ITSN2 50618
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 MOV10 4343
Affinity Capture-RNA Homo sapiens
90 HNRNPH3 3189
Co-fractionation Homo sapiens
91 GAPVD1 26130
Affinity Capture-MS Homo sapiens
92 ZCCHC10  
Affinity Capture-MS Homo sapiens
93 CLTA 1211
Proximity Label-MS Homo sapiens
94 CDR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 PRPF4 9128
Co-fractionation Homo sapiens
96 MYO6 4646
Proximity Label-MS Homo sapiens
97 IVNS1ABP  
Affinity Capture-MS Homo sapiens
98 CDK11A 728642
Affinity Capture-MS Homo sapiens
99 ZNF365  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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