Experiment description of studies that identified CROCC in exosomes

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

2 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

3 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

4 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

6 Experiment ID 196 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 197 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for CROCC

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBR2 23304 Affinity Capture-MS Homo sapiens 2 RALBP1 10928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 GAN 8139 Affinity Capture-MS Homo sapiens 4 WHAMMP3   Affinity Capture-MS Homo sapiens 5 BRDT   Affinity Capture-MS Homo sapiens 6 HDGF 3068 Co-fractionation Homo sapiens 7 SYCE1   Affinity Capture-MS Homo sapiens 8 Kif15   Affinity Capture-MS Mus musculus 9 RPA2 6118 Proximity Label-MS Homo sapiens 10 CTNNB1 1499 Affinity Capture-Western Homo sapiens Co-localization Homo sapiens 11 EZR 7430 Co-fractionation Homo sapiens 12 G3BP1 10146 Affinity Capture-MS Homo sapiens 13 TGFBRAP1 9392 Affinity Capture-MS Homo sapiens 14 SQSTM1 8878 Proximity Label-MS Homo sapiens 15 BRD2   Affinity Capture-MS Homo sapiens 16 HDAC1 3065 Affinity Capture-MS Homo sapiens 17 DDX58 23586 Affinity Capture-RNA Homo sapiens 18 XBP1   Affinity Capture-MS Homo sapiens 19 Trappc8   Affinity Capture-MS Mus musculus 20 CEP250 11190 Co-localization Homo sapiens 21 TPM1 7168 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 UBR1 197131 Affinity Capture-MS Homo sapiens 23 GIT1 28964 Affinity Capture-MS Homo sapiens 24 RPA3 6119 Proximity Label-MS Homo sapiens 25 SRBD1   Affinity Capture-MS Homo sapiens 26 SCGN   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 DTX2 113878 Proximity Label-MS Homo sapiens 28 BRD4 23476 Affinity Capture-MS Homo sapiens 29 PEX14 5195 Affinity Capture-MS Homo sapiens 30 CROCCP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 PUSL1   Affinity Capture-MS Homo sapiens 32 SCOC 60592 Affinity Capture-MS Homo sapiens 33 Magoh   Affinity Capture-MS Mus musculus 34 ZYG11B   Affinity Capture-MS Homo sapiens 35 EPS15L1 58513 Affinity Capture-MS Homo sapiens 36 KRT222 125113 Affinity Capture-MS Homo sapiens 37 TPM3 7170 Affinity Capture-MS Homo sapiens 38 RNF152 220441 Two-hybrid Homo sapiens View the network

Pathways in which CROCC is involved

No pathways found