Gene description for RNF152
Gene name ring finger protein 152
Gene symbol RNF152
Other names/aliases -
Species Homo sapiens
 Database cross references - RNF152
ExoCarta ExoCarta_220441
Vesiclepedia VP_220441
Entrez Gene 220441
HGNC 26811
UniProt Q8N8N0  
 RNF152 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RNF152
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 EXP
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 IMP
Biological Process
    protein monoubiquitination GO:0006513 IDA
    apoptotic process GO:0006915 TAS
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 IMP
    protein ubiquitination GO:0016567 TAS
    cellular response to amino acid starvation GO:0034198 IBA
    cellular response to amino acid starvation GO:0034198 IDA
    protein K63-linked ubiquitination GO:0070534 IMP
    protein K48-linked ubiquitination GO:0070936 IDA
    negative regulation of TORC1 signaling GO:1904262 IBA
    negative regulation of TORC1 signaling GO:1904262 IDA
    negative regulation of TORC1 signaling GO:1904262 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    organelle membrane GO:0031090 IMP
 Experiment description of studies that identified RNF152 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RNF152
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2W  
Two-hybrid Homo sapiens
2 CROCC 9696
Two-hybrid Homo sapiens
3 UBE2N 7334
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 EBP  
Two-hybrid Homo sapiens
5 RHEB 6009
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 UBE3A 7337
Reconstituted Complex Homo sapiens
7 C9orf40  
Affinity Capture-MS Homo sapiens
8 UBE2E1  
Two-hybrid Homo sapiens
9 TSPAN12  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
10 GJA8  
Two-hybrid Homo sapiens
11 RRAGA 10670
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 KIR2DL3  
Two-hybrid Homo sapiens
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