Gene description for RRAGA
Gene name Ras-related GTP binding A
Gene symbol RRAGA
Other names/aliases FIP-1
FIP1
RAGA
Species Homo sapiens
 Database cross references - RRAGA
ExoCarta ExoCarta_10670
Entrez Gene 10670
HGNC 16963
MIM 612194
UniProt Q7L523  
 RRAGA identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RRAGA
Molecular Function
    GTPase activity GO:0003924 IBA
    GTP binding GO:0005525 IDA
    protein binding GO:0005515 IPI
    phosphoprotein binding GO:0051219 IDA
    protein homodimerization activity GO:0042803 IDA
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    cell death GO:0008219 IDA
    regulation of autophagy GO:0010506 IBA
    cellular response to starvation GO:0009267 IBA
    metabolic process GO:0008152 IBA
    positive regulation of cytolysis GO:0045919 IDA
    cellular protein localization GO:0034613 IMP
    modulation by virus of host morphology or physiology GO:0019048 IDA
    cellular response to amino acid stimulus GO:0071230 IMP
    positive regulation of TOR signaling GO:0032008 NAS
    apoptotic process GO:0006915 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Gtr1-Gtr2 GTPase complex GO:1990131 IBA
    Golgi apparatus GO:0005794 IDA
    lysosomal membrane GO:0005765 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleus GO:0005634 IDA
    EGO complex GO:0034448 IBA
    lysosome GO:0005764 IDA
 Experiment description of studies that identified RRAGA in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RRAGA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RRAGD 58528
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 RRAGC 64121
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 NOL8  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RRAGA is involved
No pathways found





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