Gene description for FLCN
Gene name folliculin
Gene symbol FLCN
Other names/aliases BHD
FLCL
Species Homo sapiens
 Database cross references - FLCN
ExoCarta ExoCarta_201163
Vesiclepedia VP_201163
Entrez Gene 201163
HGNC 27310
MIM 607273
UniProt Q8NFG4  
 FLCN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for FLCN
Molecular Function
    enzyme inhibitor activity GO:0004857 IDA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    protein-containing complex binding GO:0044877 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    in utero embryonic development GO:0001701 ISS
    cell-cell junction assembly GO:0007043 ISS
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    cellular response to starvation GO:0009267 IDA
    positive regulation of autophagy GO:0010508 IDA
    positive regulation of autophagy GO:0010508 IMP
    hemopoiesis GO:0030097 ISS
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IBA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISS
    TOR signaling GO:0031929 IEA
    regulation of TOR signaling GO:0032006 ISS
    negative regulation of TOR signaling GO:0032007 ISS
    positive regulation of TOR signaling GO:0032008 IDA
    positive regulation of TOR signaling GO:0032008 ISS
    lysosome localization GO:0032418 IDA
    cellular response to amino acid starvation GO:0034198 IDA
    negative regulation of Rho protein signal transduction GO:0035024 IMP
    intracellular signal transduction GO:0035556 IDA
    positive regulation of apoptotic process GO:0043065 ISS
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IEA
    negative regulation of glycolytic process GO:0045820 IDA
    regulation of Ras protein signal transduction GO:0046578 IDA
    epithelial cell proliferation GO:0050673 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISS
    ERK1 and ERK2 cascade GO:0070371 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    cell proliferation involved in kidney development GO:0072111 IEA
    energy homeostasis GO:0097009 ISS
    intrinsic apoptotic signaling pathway GO:0097193 IEA
    negative regulation of cold-induced thermogenesis GO:0120163 ISS
    negative regulation of cell proliferation involved in kidney development GO:1901723 ISS
    negative regulation of post-translational protein modification GO:1901874 IEA
    negative regulation of brown fat cell differentiation GO:1903444 ISS
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IDA
    negative regulation of lysosome organization GO:1905672 IDA
    regulation of pro-B cell differentiation GO:2000973 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 TAS
    lysosomal membrane GO:0005765 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    cilium GO:0005929 IDA
    cilium GO:0005929 TAS
    midbody GO:0030496 IDA
    cell-cell contact zone GO:0044291 IDA
    mitotic spindle GO:0072686 IDA
    FNIP-folliculin RagC/D GAP GO:1990877 IDA
 Experiment description of studies that identified FLCN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FLCN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 WDFY3 23001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
4 PGK2 5232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LAMTOR4 389541
Affinity Capture-MS Homo sapiens
7 FNIP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CCT8 10694
Affinity Capture-MS Homo sapiens
10 AHSA1 10598
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 RRAGA 10670
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
14 TP53BP1 7158
Affinity Capture-MS Homo sapiens
15 EGLN3  
Affinity Capture-MS Homo sapiens
16 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 AGAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LRRC25  
Affinity Capture-MS Homo sapiens
19 CTAG1B  
Affinity Capture-MS Homo sapiens
20 BANP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PRKAA2 5563
Affinity Capture-MS Homo sapiens
22 CDC37 11140
Affinity Capture-Western Homo sapiens
23 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
24 TSPAN17 26262
Affinity Capture-Western Homo sapiens
25 NR3C1 2908
Proximity Label-MS Homo sapiens
26 RRAGD 58528
Affinity Capture-Western Homo sapiens
27 CCT4 10575
Affinity Capture-MS Homo sapiens
28 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 POLR3K  
Affinity Capture-MS Homo sapiens
30 VTA1 51534
Affinity Capture-MS Homo sapiens
31 FNIP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 RRAGB 10325
Affinity Capture-Western Homo sapiens
34 APP 351
Reconstituted Complex Homo sapiens
35 CCT7 10574
Affinity Capture-MS Homo sapiens
36 AGAP3 116988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MAP1LC3B 81631
Reconstituted Complex Homo sapiens
38 FAM120B 84498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 C12orf49  
Negative Genetic Homo sapiens
40 BTBD1  
Affinity Capture-MS Homo sapiens
41 GABARAP 11337
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 CTAG2  
Affinity Capture-MS Homo sapiens
43 RAB7A 7879
Proximity Label-MS Homo sapiens
44 YWHAH 7533
Affinity Capture-MS Homo sapiens
45 RPL10 6134
Affinity Capture-MS Homo sapiens
46 SF3B1 23451
Affinity Capture-MS Homo sapiens
47 LDLR 3949
Positive Genetic Homo sapiens
48 ULK1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
49 PTGES3 10728
Affinity Capture-Western Homo sapiens
50 Fem1b  
Affinity Capture-MS Mus musculus
51 HIBCH 26275
Affinity Capture-MS Homo sapiens
52 SF3B3 23450
Affinity Capture-MS Homo sapiens
53 SEMA4C 54910
Affinity Capture-MS Homo sapiens
54 STIP1 10963
Affinity Capture-Western Homo sapiens
55 HSPA1B 3304
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 RRAGC 64121
Affinity Capture-Western Homo sapiens
57 ZNF609  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
59 PPP5C 5536
Affinity Capture-Western Homo sapiens
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