Gene description for CTU2
Gene name cytosolic thiouridylase subunit 2 homolog (S. pombe)
Gene symbol CTU2
Other names/aliases C16orf84
NCS2
UPF0432
Species Homo sapiens
 Database cross references - CTU2
ExoCarta ExoCarta_348180
Vesiclepedia VP_348180
Entrez Gene 348180
HGNC 28005
UniProt Q2VPK5  
 CTU2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CTU2
Molecular Function
    tRNA binding GO:0000049 IEA
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    sulfurtransferase activity GO:0016783 IBA
Biological Process
    tRNA wobble uridine modification GO:0002098 NAS
    tRNA wobble position uridine thiolation GO:0002143 IBA
    protein urmylation GO:0032447 IEA
    tRNA thio-modification GO:0034227 NAS
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified CTU2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CTU2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB8B 51762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ZNF414  
Affinity Capture-MS Homo sapiens
4 CCNDBP1 23582
Affinity Capture-MS Homo sapiens
5 POLM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SLC5A5 6528
Affinity Capture-MS Homo sapiens
7 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RRAGA 10670
Affinity Capture-MS Homo sapiens
9 CTU1 90353
Affinity Capture-MS Homo sapiens
10 THUMPD3 25917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CIAO1 9391
Affinity Capture-MS Homo sapiens
12 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LDLRAD1  
Affinity Capture-MS Homo sapiens
14 HCST  
Affinity Capture-MS Homo sapiens
15 S100A6 6277
Affinity Capture-MS Homo sapiens
16 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 C1QA 712
Affinity Capture-MS Homo sapiens
18 NFKBIA  
Affinity Capture-MS Homo sapiens
19 DNAL4  
Affinity Capture-MS Homo sapiens
20 AJUBA  
Affinity Capture-MS Homo sapiens
21 CUL3 8452
Affinity Capture-MS Homo sapiens
22 ZNF444  
Affinity Capture-MS Homo sapiens
23 CEP41  
Affinity Capture-MS Homo sapiens
24 SCPEP1 59342
Affinity Capture-MS Homo sapiens
25 ZMAT5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PAFAH2 5051
Affinity Capture-MS Homo sapiens
27 FAXC  
Affinity Capture-MS Homo sapiens
28 TINAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 VASP 7408
Affinity Capture-MS Homo sapiens
30 FAM96B 51647
Affinity Capture-MS Homo sapiens
31 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
32 EGFR 1956
Negative Genetic Homo sapiens
33 FAM219A 203259
Affinity Capture-MS Homo sapiens
34 FAM19A3  
Affinity Capture-MS Homo sapiens
35 RRAS 6237
Affinity Capture-MS Homo sapiens
36 CYP4F8  
Affinity Capture-MS Homo sapiens
37 PBXIP1 57326
Affinity Capture-MS Homo sapiens
38 OGT 8473
Reconstituted Complex Homo sapiens
39 ACBD6 84320
Affinity Capture-MS Homo sapiens
40 PRAMEF9  
Affinity Capture-MS Homo sapiens
41 SPHK1 8877
Affinity Capture-MS Homo sapiens
42 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KCTD17 79734
Affinity Capture-MS Homo sapiens
44 FLII 2314
Affinity Capture-MS Homo sapiens
45 PFDN4 5203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ECM1 1893
Affinity Capture-MS Homo sapiens
47 KRAS 3845
Negative Genetic Homo sapiens
48 APEX2  
Affinity Capture-MS Homo sapiens
49 LBHD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CEP72  
Affinity Capture-MS Homo sapiens
51 UROS 7390
Affinity Capture-MS Homo sapiens
52 CHIT1  
Affinity Capture-MS Homo sapiens
53 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RAB20 55647
Affinity Capture-MS Homo sapiens
55 RGS20 8601
Affinity Capture-MS Homo sapiens
56 EP300 2033
Affinity Capture-MS Homo sapiens
57 WISP2 8839
Affinity Capture-MS Homo sapiens
58 PROSER2 254427
Affinity Capture-MS Homo sapiens
59 RAP2A 5911
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CTU2 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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